Human Club cell protein(CC16、CC10、子宫珠蛋白、尿蛋白1或克拉拉细胞分泌)是分泌珠蛋白家族的成员,是非连接的分泌产物。细支气管Clara细胞,它的功能还有待充分阐明,但令人信服的数据表明其作为免疫调节剂和抗炎剂的作用。Club cell protein抑制磷脂酶A2活性以及干扰素γ信号传导和Th1与Th2淋巴细胞调节。血清、血浆和支气管肺泡灌洗液中的Clara细胞蛋白浓度测定。
自1994年以来在众多研究中使用的洗液。在血清中,它的增加与年龄和石棉、氯化氮和臭氧暴露。更高水平的CC16在结节病、肺纤维化和高PEEP通气患者。血清减少在吸烟者和慢性病患者的肺切除术后发现CC16水平。阻塞性肺病、哮喘或二氧化硅暴露。患此病的胎儿羊水中也发现CC16浓度降低。各种机制引起的肺发育不良(膈疝、糖尿病)嗜好、特纳和唐氏综合征)。在胸腔积液中,CC16浓度出现。与肺中CC16高水平的扩散有关。胸膜充血。基于以上报道,Club cell protein可能是肺部疾病和损伤有前景的诊断标志物。
今天给大家带来了艾美捷代理品牌BioVendor公司的一款备受好评的Clara细胞蛋白(CC16)Elisa 试剂盒:
货号 | 产品名称 | 规格 |
RD191022200 | Club Cell Protein (CC16) Human ELISA 人Clara细胞蛋白(CC16)Elisa 试剂盒 | 96 wells(1 kit) |
以上产品仅用于科研,不能用于医疗诊断~
检测步骤:
检测范围和灵敏度:
指标 | 检测范围 | 灵敏度 |
CC16 | 1.57–50 ng/ml | 46 pg/ml |
精确度:
组内差异(n=8)
Sample | Mean (ng/ml) | SD (ng/ml) | CV (%) |
1 | 14.28 | 0.55 | 3.82 |
2 | 5.31 | 0.16 | 2.96 |
组间差异(n=4)
Sample | Mean (ng/ml) | SD (ng/ml) | CV (%) |
1 | 5,37 | 0.22 | 4.09 |
2 | 7.75 | 0.49 | 6.36 |
3 | 16.59 | 0.63 | 3.78 |
不同体液样本中CC16的典型浓度:
样本类型 | 平均值 (ng/ml) | 范围 (ng/ml) |
血清 | 12.6 | 3.7 – 23.2 |
尿液 | 18.7 | 0.2 – 88.6 |
精液 | 1 030.0 | 145 – 8 600 |
BAL | 1 360.0 | 154 – 4 300 |
滑液 | 9.1 | 2.8 – 16.4 |
胸膜液 | 11.4 | 0.7 – 32.8 |
脑脊液 | 0.5 | 0 – 5.7 |
胃液 | 185.0 | 0 – 1 220 |
胆汁 | 0.7 | 0 – 2.3 |
详情参见:
Shijubo N., Kawabata I., Sato N., Itoh Y.: Clinical Aspects of Clara Cell 10-kDa Protein/ Uteroglobin (Secretoglobin 1A1), Current Pharmaceutical Design, 9, 1139-1149, (2003)
标准曲线:
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