【方法大全】Adenovirus Titer，腺病毒滴度快速检测

【前情提示】

1. 慢病毒滴度检测

2.腺相关病毒（AAV）滴度检测

腺病毒(Adenovirus)是一种没有包膜的直径为90-100 nm的颗粒，由252个壳粒呈20面体排列构成。每个壳粒的直径为7～9 nm。衣壳里是线状双链DNA分子，约含4.7kb。腺病毒最常引起呼吸道疾病，从普通感冒到肺炎、哮吼和支气管炎。根据类型，腺病毒可引起其他疾病，如胃肠炎、结膜炎、膀胱炎，以及较少见的神经系统疾病。

可能很多人第一次听到腺病毒还是因为咱们陈薇院士团队开发的，唯一可采用单针接种程序的疫苗：腺病毒载体疫苗。腺病毒载体疫苗，是指以腺病毒作为载体，将保护性抗原基因重组到腺病毒基因组中，使用能表达保护性抗原基因的重组腺病毒制成的疫苗。它是一种不同于传统疫苗和mRNA疫苗的另外一条技术路线！

那么如何真正准确可靠地检测腺病毒（adenovirus）滴度呢？是从事腺病毒科研和疫苗研发的工作者非常关心的问题！

作为专业的生命科学医药原料供应商，艾美捷科技为您推荐：下面我们就来聊下几种常见的腺病毒滴度的检测方法。

① VPK-110, 腺病毒滴度ELISA检测试剂盒

提供了一个快速、完整的系统，以功能滴度病毒感染。该检测方法识别所有41种血清型腺病毒，可用于任何能在hek293细胞中扩增的腺病毒系统。

VPK-110 标曲

发表文章，VPK-110, 腺病毒滴度ELISA检测试剂盒：

1.Teves, M.E. et al. (2020). Human DENND1A.V2 Drives Cyp17a1 Expression and Androgen Production in Mouse Ovaries and Adrenals.Int J Mol Sci.21(7). pii: E2545. doi: 10.3390/ijms21072545.

2.Guo, Y. et al. (2019). Menstrual blood-derived stem cells as delivery vehicles for oncolytic adenovirus virotherapy for colorectal cancer.Stem Cells Dev.doi: 10.1089/scd.2018.0222.

3.Wu, Y. et al (2017). Immune efficacy of an adenoviral vector-based swine influenza vaccine against antigenically distinct H1N1 strains in mice.Antiviral Research.147: 29-36.

4.Kirshmer, N. et al. (2016). TRPC4a and TRPC4 similarly affect neonatal cardiomyocyte survival during chronic GPCR stimulation.PLoS One11:e0168446.

5.García-Pascual, C. M. et al. (2015). Evaluation of the potential therapeutic effects of a double-stranded RNA mimiccomplexed withpolycationsin an experimental mouse model of endometriosis.Fertil Steril.doi:10.1016/j.fertnstert.2015.07.1147.

② VPK-109, 腺病毒滴度IHC检测试剂盒

提供了一个快速、完整的系统，以功能滴度病毒感染。该检测方法识别所有41种血清型腺病毒，可用于任何能在hek293细胞中扩增的腺病毒系统。

发表文章，VPK-109, 腺病毒滴度IHC检测试剂盒：

1.McQuerry, J.A. et al. (2021). Tepoxalin increases chemotherapy efficacy in drug-resistant breast cancer cells overexpressing the multidrug transporter gene ABCB1.Transl Oncol.14(10):101181. doi: 10.1016/j.tranon.2021.101181.

2.Tournier, B.B. et al. (2021). Amyloid and Tau Induce Cell Death Independently of TSPO Polymerization and Density Changes.ACS Omega. doi: 10.1021/acsomega.1c01678.

3.Oliveira, E.R.A. et al (2021). Intracellular Sequestration of the NKG2D Ligand MIC B by Species F Adenovirus.Viruses.13(7):1289. doi: 10.3390/v13071289.

4.Borrás, T. et al. (2020). Generation of a Matrix Gla (Mgp) floxed mouse, followed by conditional knockout, uncovers a new Mgp function in the eye.Sci Rep.10(1):18583. doi: 10.1038/s41598-020-75031-7.

5.Wei, W.Z. et al. (2020). Diversity Outbred Mice Reveal the Quantitative Trait Locus and Regulatory Cells of HER2 Immunity.J Immunol. doi: 10.4049/jimmunol.2000466.

6.Robles-Rodríguez, O.A. et al. (2020). Antitumor effect of adenoviruses expressing mutant nononcogenic E7 versions from HPV-16 fused to calreticulin.JBUON.25(1): 543-548.

7.Rollier, C.S. et al. (2020). Modification of Adenovirus vaccine vector-induced immune responses by expression of a signalling molecule.Sci Rep.10(1):5716. doi: 10.1038/s41598-020-61730-8.

8.Lokhandwala, S. et al. (2019). Adenovirus-vectored African Swine Fever Virus antigen cocktails are immunogenic but not protective against intranasal challenge with Georgia 2007/1 isolate.Veterinary Microbiology.235:10-20. doi:10.1016/j.vetmic.2019.06.006.

9.Sang, Y. et al. (2019). Ileal transcriptome analysis in obese rats induced by high-fat diets and an adenoviral infection.Int J Obes (Lond). doi: 10.1038/s41366-019-0323-2.

10.Hao, J. et al. (2018). Prostate Cancer-Specific of DD3-driven Oncolytic Virus-harboring mK5 Gene.Open Med (Wars).14: 1-9. doi: 10.1515/med-2019-0001.

③ VPK-106, 腺病毒核酸滴度检测试剂盒

提供了一个快速的方法来测量你的腺病毒的病毒核酸含量。此试验可在病毒纯化之前或之后进行。

发表文章，VPK-106, 腺病毒滴度IHC检测试剂盒：

1.Stone, A.V. et al. (2020). Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus.Cartilage. doi: 10.1177/1947603520958143.

2.Hossain, E. et al. (2020). Advantages of Using Paclitaxel in Combination with Oncolytic Adenovirus Utilizing RNA Destabilization Mechanism.Cancers (Basel).12(5). pii: E1210. doi: 10.3390/cancers12051210.

3.Habiba, U. et al. (2020). Cisplatin Relocalizes RNA Binding Protein HuR and Enhances the Oncolytic Activity of E4orf6 Deleted Adenovirus.Cancers.12:809. doi: 10.3390/cancers12040809.

4.Oser, M.G. et al. (2019). The KDM5A/RBP2 histone demethylase represses NOTCH signaling to sustain neuroendocrine differentiation and promote small cell lung cancer tumorigenesis.Genes Dev. doi: 10.1101/gad.328336.119.

5.Yanagawa-Matsuda, A. et al. (2019). Oncolytic potential of an E4-deficient adenovirus that can recognize the stabilization of AU-rich element containing mRNA in cancer cells.Oncol Rep.41(2):954-960. doi: 10.3892/or.2018.6865.

6.Yan, Y. et al. (2016). HIV-1 Vpr increases HCV replication through VprBP in cell culture.Virus Res.doi:10.1016/j.virusres.2016.07.007.

7.Xie, Y. et al. (2016). A recombinantadenovirus expressing P12A and 3C protein of the type O foot-and-mouth disease virus stimulates systemic and mucosal immune responses in mice.BioMed Res Int. doi:10.1155/2016/7849203.

8.Ran, L. et al. (2016). Delivery of oncolytic adenovirus into the nucleus of tumorigenic cells by umor microparticles for virotherapy. Biomaterials. 89:56-66.

9.Nuche-Berenguer, B. et al. (2016). The p21-activated kinase, PAK2, is important in the activation of numerous pancreatic acinar cell signaling Cascades and in the onset of early pancreatitis events. Biochim Biophys Acta. doi:10.1016/j.bbadis.2016.02.008.

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