腺相关病毒（AAV）滴度快速检测试剂盒

【前情提示】慢病毒滴度检测

腺相关病毒（adeno-associatedvirus，AAV）,也称腺伴随病毒,属于微小病毒科依赖病毒属,是目前发现的一类结构最简单的单链DNA缺陷型病毒，需要辅助病毒（通常为腺病毒）参与复制。AAV基因组为4.8kb的线状单链DNA；AAV免疫原性低，基因持续表达半年以上；AAV具有多种血清型，可以特异性靶向感染不同的组织或器官，是动物活体基因转导的首选工具！

腺相关病毒血清型众多（12种），不同血清型对不同组织亲和性不同。如何选择呢？（如下图）

通过病毒载体传递基因的一个特殊挑战是病毒滴度的准确测量。传统上，AAV颗粒是通过DNA斑点印迹或类似方法测量的。这些方法非常耗时，并且存在高度的测定间变异性。对于高度纯化的病毒样本，260nm处的吸光度已用于估计病毒颗粒的总数。然而，这种方法不能用于未纯化的病毒上清液，因为它包含的其他成分会导致260nm的吸光度。通过使用仅与AAV完整颗粒反应的抗体开发了一种ELISA方法；然而，这种方法测量所有AAV颗粒，包括那些缺乏基因组DNA的颗粒。

那么如何真正准确可靠地检测腺相关病毒（AAV）滴度呢？作为专业的生命科学医药原料供应商，艾美捷科技为您推荐：众多高分文章引用的，腺相关病毒（AAV）滴度检测试剂盒（适用于任何血清型的纯化AAV，以及未纯化的AAV-2和AAV-DJ型）

* 本产品仅适用于科研用途.

腺相关病毒（AAV）滴度检测试剂盒原理与标曲展示：

腺相关病毒（AAV）滴度检测试剂盒的Nature结果鉴赏：

Nat Neurosci. doi: 10.1038/s41593-020-0674-y.

近期发表文章：

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3.Lebeau, P.F. et al. (2020). The loss-of-function PCSK9Q152H variant increases ER chaperones GRP78 and GRP94 and protects against liver injury. J Clin Invest. doi: 10.1172/JCI128650.

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5.Rai, R. et al. (2020). Targeted gene correction of human hematopoietic stem cells for the treatment of Wiskott - Aldrich Syndrome. Nat Commun. 11(1):4034. doi: 10.1038/s41467-020-17626-2.

6.Zeng, J. et al (2020). The Zika Virus Capsid Disrupts Corticogenesis by Suppressing Dicer Activity and miRNA Biogenesis. Cell Stem Cell. S1934-5909(20)30350-7. doi: 10.1016/j.stem.2020.07.012.

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11.Xu, Y. et al. (2020). Diabetic nephropathy execrates epithelial-to-mesenchymal transition (EMT) via miR-2467-3p/Twist1 pathway. Biomed Pharmacother. 125:109920. doi: 10.1016/j.biopha.2020.109920.

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13.Niranjan, N. et al. (2019). Sarcolipin overexpression impairs myogenic differentiation in Duchenne muscular dystrophy. Am J Physiol Cell Physiol. doi: 10.1152/ajpcell.00146.2019.

14.Ferretti, V. et al. (2019). Oxytocin Signaling in the Central Amygdala Modulates Emotion Discrimination in Mice. Curr Biol. pii: S0960-9822(19)30499-3. doi: 10.1016/j.cub.2019.04.070.

15.Hasan, M.T. et al. (2019). A Fear Memory Engram and Its Plasticity in the Hypothalamic Oxytocin System. Neuron. pii: S0896-6273(19)30386-1. doi: 10.1016/j.neuron.2019.04.029.

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17.Nakamura, M. et al. (2019). Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy. Cell Metab. pii: S1550-4131(19)30005-1. doi: 10.1016/j.cmet.2019.01.005.

18.Tseng, S.J. et al. (2018). Targeting Tumor Microenvironment by Bioreduction-Activated Nanoparticles for Light-Triggered Virotherapy. ACS Nano. 12(10):9894-9902. doi: 10.1021/acsnano.8b02813.

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20.Menon, R. et al. (2018). Oxytocin Signaling in the Lateral Septum Prevents Social Fear during Lactation. Curr Biol. 28(7):1066-1078.e6. doi: 10.1016/j.cub.2018.02.044.

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27.Park, H. Y. et al. (2016). Gadd45β ameliorates L-DOPA-induced dyskinesia in a Parkinson's disease mouse model. Neurobiol Dis. 89:169-179.

28.Tran, L. & Keele, N. B. (2016). CaMKIIα knockdown decreases anxiety in the open field and low serotonin-induced upregulation of GluA1 inthe basolateral amygdala. Behav Brain Res. 303:152-159.

29.Chen, W. et al. (2016). The silencing of cathepsin K used in gene therapy for periodontal disease reveals the role of cathepsin K in chronic infection and inflammation. J Periodontal Res.doi:10.1111/jre.12345.

30.Orabi, A. I. et al. (2015). Dynamic imaging of pancreatic NF-κB activation in live mice using AAV infusion and ioluminescence. J Biol Chem. doi:10.1074/jbc.M115.647933

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