SouthernBiotech丨明胶底玻片的多种应用及文献参考

SouthernBiotech的明胶底玻片已经过仔细清洁，并用0.1%明胶和硫酸钾铬的溶液进行了底涂。涂有涂层的载玻片尺寸为25毫米x 75毫米，带有磨砂端用于标记。为了获得合适的附着力，SouthernBiotech建议厚度小于100μm的部分。

艾美捷SouthernBiotech丨明胶底玻片（SLD01-CS）的应用：

冰冻切片——文献3,6中报道

自由漂浮冷冻切片——文献4,5,7,8中报道

树脂嵌入部分——文献2中报道

TUNEL——文献1中报道

图：自由漂浮Bcs1lc.232A>Gmouse脑皮质冷冻切片用抗GFAP染色，然后二次试剂DAB，并安装在明胶载玻片上（SB目录号SLD01）。图片来自Tegelberg S、Toma?id N、Kallj?rvi J、Purhonen

J、 埃尔默

SouthernBiotech丨明胶底玻片参考文献：

1. Lee T, Zhang X, Dhar S, Faas H, Lippard SJ, Jasanoff A. In vivo imaging with a cell-permeable porphyrin-based MRI contrast agent. Chem Biol.

2010;17:665-73. (TUNEL)

2. Curcio CA, Messinger JD, Sloan KR, Mitra A, McGwin G, Spaide RF. Human chorioretinal layer thicknesses measured in macula-wide, highresolution histologic sections. Invest Ophthalmol Vis Sci. 2011;52:3943-54. (Resin embedded sections)

3. Deane EC, Ilie AE, Sizdahkhani S, Gupta MD, Orlowski J, McKinney RA. Enhanced recruitment of endosomal Na+/H+ exchanger NHE6 into dendritic spines of hippocampal pyramidal neurons during NMDA receptor-dependent long-term potentiation. J Neurosci. 2013;33:595-610. (Frozen sections)

4. Hodor A, Palchykova S, Baracchi F, Noain D, Bassetti CL. Baclofen facilitates sleep, neuroplasticity, and recovery after stroke in rats. Ann Clin Transl Neurol. 2014;1:765-77. (Free floating frozen sections)

5. Kim KY, Scholl ES, Liu X, Shepherd A, Haeseleer F, Lee A. Localization and expression of CaBP1/caldendrin in the mouse brain. Neuroscience.2014;268:33-47. (Free floating frozen sections)

6. Adjei S, Wesson DW. Laminar and spatial localization of the islands of Calleja in mice. Neuroscience. 2015;287:137-43. (Frozen sections)

7. Tegelberg S, Toma?i? N, Kallij?rvi J, Purhonen J, Elmér E, Lindberg E, et al. Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model. Orphanet J Rare Dis. 2017;12:73. (Free floating frozen sections)

8. Morabito MV, Ravussin Y, Mueller BR, Skowronski AA, Watanabe K, Foo KS, et al. Weight perturbation alters leptin signal transduction in a regionspecific manner throughout the brain. PLoS One. 2017;12(1): e0168226. (Free floating frozen sections)

SouthernBiotech 致力于高质量、高亲和纯化的二抗的生产、纯化和标记。  同时，SBA还提供各种亲和纯化的多克隆和单克隆抗体，其中包括：荧光素和酶标记物，低内毒素/不含叠氮化物（LE/AF）制剂，F(ab)和 F(ab')2片段化抗体，此外还有纯化的免疫球蛋白、血清以及细胞外基质蛋白等产品。艾美捷科技是SouthernBiotech的中国代理商，为科研工作者提供优质的产品与服务。