TG2重组蛋白/Recombinant TG2英文说明书:
Art. No.:T122
Synonym:Tissue-type Transglutaminase, TG2, TGase 2, protein-glutamine-γ-glutamyltransferase, His6-rhTG2
Molecular Weight:78 kDa
Source:Recombinantly produced in insect cells
Purity:> 95% [SDS-PAGE and Coomassie staining]
The gel shows a single dominant protein band at ~80 kDa.
Appearance:White lyophilized solid
Reagents:The Transglutaminase is lyophilized from 10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5 mM DTT, 1 mM EDTA, 189 mg/mL Maltodextrin.
Reconstitution:Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
Application:The recombinant antigen is meant for solid (ELISA and immuno blot) and fluid phase diagnostic assays. The protein is bound by human type IgA and IgG (auto) antibodies.
Coating:Dilute with your coating buffer to an appropriate concentration e.g. 1 μg/ml. Please notice that coating conditions have to be evaluated carefully.
Storage:T122 is stable for at least two years if stored at ≤ -20°C.
Delivery is possible at ambient temperature
Note:Intended for research use only, not for use in human, therapeutic or diagnostic applications.
艾美捷TG2重组蛋白/Recombinant TG2中文说明书:
产品编号:T122
同义词:组织型转谷氨酰胺酶,TG2,TGase 2,蛋白谷氨酰胺γ-谷氨酰转移酶,His6-rhTG2
分子量:78 kDa
来源:在昆虫细胞中重组生产
纯度:
95% [通过SDS-PAGE和Coomassie染色检测]
凝胶显示一个主要的蛋白条带,大约在80 kDa。
外观:白色冻干固体
试剂:转谷氨酰胺酶是从10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5 mM DTT, 1 mM EDTA, 189 mg/mL 麦芽糊精中冻干得到的。
复溶:向冻干粉末的小瓶中加入与蛋白质冻干前相同的体积的水(见分析证书)。轻轻旋转小瓶直到固体溶解。复溶后,溶液应分装后冷冻储存。短期储存时,请保持在冰上冷却。
应用:重组抗原适用于固相(酶联免疫吸附试验ELISA和免疫印迹)和液相诊断检测。该蛋白可被人IgA和IgG(自身)抗体结合。
包被:用您的包被缓冲液稀释至适当浓度,例如1 μg/ml。请注意,包被条件需要仔细评估。
储存:如果储存在≤ -20°C,T122至少稳定两年。可以在常温下交付。
注意:仅供研究使用,不适用于人体、治疗或诊断应用。
仅供研究使用,不适用于人体、治疗或诊断应用。
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