丙酮酸(Pyruvate)定量分析试剂盒检测精准可靠！

       丙酮酸盐在糖酵解等过程中有重要作用： 它可以在细胞溶质葡萄糖进行糖酵解的过程中，由磷酸烯醇式丙酮酸产生。丙酮酸盐可以继续进入柠檬酸循环继续氧化分解，转化为碳水化合物或通过乙酰CoA 转化为脂肪酸。高水平的丙酮酸盐提示肝脏疾病和遗传性代谢紊乱。丙酮酸盐也被用来作为减肥刺激剂。在无氧呼吸中丙酮酸可以转化为乳酸或者乙醇。

       作为专业性的产品供应公司，艾美捷科技为您推荐，可同时用于比色法和荧光法的丙酮酸定量分析试剂盒：K609-100

       * 细胞或组织样品需做去蛋白处理（会消耗丙酮酸），详情请见说明书
       * 基于客户仪器设备，自行选择比色法或者荧光法即可

       丙酮酸检测分析试剂盒原理：

       丙酮酸盐检测试剂盒提供了一种简单、直接和可自动化操作的程序来测量不同生物学标本中丙酮酸的含量。适用标本类型包括：食物、细胞、培养基和发酵培养基。丙酮酸盐检测试剂盒基本原理如下：丙酮酸盐被丙酮酸酶氧化，在酶反应过程中产生颜色（λ= 570 nm）或者荧光（Ex/Em = 535/587 nm），可使用酶标仪或荧光计检测到。光的强度与丙酮酸含量是成正比的，因此通过检测光的强度即可精确的得到丙酮酸的含量。试剂盒检测范围为1-10 uM丙酮酸。

《比色法和荧光法标准曲线展示》

       丙酮酸检测分析试剂盒发表文章：

       1.Alenzi, Mohammed et al. (2017) Antiurolithic effect of olive oil in a mouse model of ethylene glycol-induced urolithiasis, Investig Clin Urol. 2017 May;58(3):210-216.
       2.Chem et al., Mitochondrial Citrate Transporter-dependent Metabolic Signature in the 22q11.2 Deletion Syndrome. J. Biol. Chem., Sep 2015; 290: 23240 - 23253.
       3.Chen et al., Cadmium toxicity induces ER stress and apoptosis via impairing energy homoeostasis in cardiomyocytes. Biosci. Rep., Jun 2015; 35: e00214.
       4.Chettimada et al., Hypoxia-induced glucose-6-phosphate dehydrogenase overexpression and -activation in pulmonary artery smooth muscle cells: implication in pulmonary hypertension.Am J Physiol Lung Cell Mol Physiol, Feb 2015; 308: L287 - L300.
       5.Cho, S.C. et. al. DDS, 4,4'-diaminodiphenylsulfone, extends organismic lifespan. PNAS, Nov 2010; 107: 19326 - 19331.
       6.Luitpold Fried et al., Identification of a Target Gene and Activating Stimulus for the YpdA/YpdB Histidine Kinase/Response Regulator System in Escherichia coli. J. Bacteriol., Feb 2013; 195: 807 - 815.
       7.McNeely, K. et al. Redirecting Reductant Flux into Hydrogen Production via Metabolic Engineering of Fermentative Carbon Metabolism in a Cyanobacterium. Appl. Envir. Microbiol., 2010; 10.1128/AEM.00862-10
       8.Min You et al., Ethanol administration exacerbates the abnormalities in hepatic lipid oxidation in genetically obese mice. Am J Physiol Gastrointest Liver Physiol, Jan 2013; 304: G38 - G47
       9.Müller et al., Engineering of Ralstonia eutropha H16 for Autotrophic and Heterotrophic Production of Methyl Ketones. Appl. Envir. Microbiol., Jul 2013; 79: 4433 - 4439.
       10.Negi et al., A Carbon-Nitrogen Lyase from Leucaena leucocephala Catalyzes the First Step of Mimosine Degradation. Plant Physiology, Feb 2014; 164: 922 - 934.
       11.Odet, F. et. al. Lactate Dehydrogenase C and Energy Metabolism in Mouse Sperm. Biol Reprod, Sep 2011; 85: 556 - 564.
       12.Palamiuc et al., A metabolic switch toward lipid use in glycolytic muscle is an early pathologic event in a mouse model of amyotrophic lateral sclerosis. EMBO Mol Med., May 2015; 7: 526 - 546.
13.Park et al., Changes in Pyruvate Metabolism Detected by Magnetic Resonance Imaging Are Linked to DNA Damage and Serve as a Sensor of Temozolomide Response in Glioblastoma Cells.Cancer Res., Dec 2014; 74: 7115 - 7124.

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