PNA Bio-PNA FISH测试：与目标DNA以高灵敏度和特异性杂交

PNA FISH探针因其化学结构的电中性，能够与目标DNA以高灵敏度和特异性杂交。这一特性使得PNA探针在应用到FISH（荧光原位杂交）时更加高效且有用，即使在低浓度下也是如此。杂交发生得很快（在几个小时内），并且背景噪声明显降低。

PNA Bio提供端粒、着丝粒和CAG重复序列探针作为目录项目，以及定制PNA探针合成服务。

TelC是一种富C的端粒探针，用于领先链（TAACCC的重复序列），而TelG是一种富G的端粒探针，用于滞后链（TTAGGG的重复序列）。TelC和TelG PNA端粒探针均可用于人类、小鼠和大鼠染色体。

CENPB探针是着丝粒蛋白B（CENP-B）的结合位点，CENP-B是主要的着丝粒特异性蛋白之一。探针序列为ATTCGTTGGAAACGGGA，它染色所有人类和小鼠着丝粒的小卫星，除了Y染色体。

Cent探针特异性地针对人类α卫星着丝粒，不与小鼠发生反应。探针序列为AAACTAGACAGAAGCAT。

艾美捷PNA Bio-PNA FISH探针：

PNA Bio-PNA FISH探针文献参考：

Integrity of the human centromere DNA repeats is protected by CENP-A, CENP-C, & CENP-T . Giunta S & Funabiki H. (2017) Proc Natl Acad Sci USA 114(8):1928-1933.

Comprehensive characterization of neutrophil genome topology. Zhu Y et al. (2017) Genes & Development. 31(2):141–153.

Long telomeres protect against age-dependent cardiac disease caused by NOTCH1 haploinsufficiency. Theodoris CV et al. (2017) J Clinical Investigation 127(5):1683-1688.

Quantification of telomere features in tumor tissue sections by an automated 3D imaging-based workflow. Gunkel M et al (2017) Methods 114:60-73.

Telomere Replication Stress Induced by POT1 Inactivation Accelerates Tumorigenesis. Pinzaru AM et al. (2016) Cell Rep 15(10):2170-2184.

Regulation of the Human Telomerase Gene TERT by Telomere Position Effect—Over Long Distances (TPE-OLD): Implications for Aging and Cancer. Kim W et al (2016) pLoS Biology 2000016.

Dysfunctional telomeres induce p53‐dependent and independent apoptosis to compromise cellular proliferation and inhibit tumor formation. Wang Y et al (2016) Aging Cell 15(4): 646–660.

ATRX loss promotes tumor growth and impairs nonhomologous end joining DNA repair in glioma. Koschmann C et al (2016) Sci Transl Med. 8(328):328.

Oocyte Polarization Is Coupled to the Chromosomal Bouquet, a Conserved Polarized Nuclear Configuration in Meiosis. Elkouby YM et al. (2016) PLoS Biol. 14(1):e1002335.

Cell Death During Crisis Is Mediated by Mitotic Telomere Deprotection. Hayashi MT et al (2015)Nature 522(7557):492–496.

SMARCAL1 maintains telomere integrity during DNA replication. Poole LA et al. (2015) Proc Natl Acad Sci U S A. 112(48):14864-14869.

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