Jena bioscience--5-Methyl-CTP在表观遗传学中的作用

Jena bioscience--5-Methyl-CTP的应用：

1、修饰碱基的功能影响[1-5]

2、PCR中的掺入[3]。

Cat. No. Amount

NU-1138S 10 ?l (100 mM)

NU-1138L 5 x 10 ?l (100 mM)

艾美捷5-Methyl-CTP：

货号：NU-1138L

运输：使用凝胶包装运输

储存条件：需在-20°C下储存，短期（累计不超过1周）暴露在室温下是可能的。

保质期：自交货日期起12个月

分子式：C10H18N3O14P3（游离酸）

分子量：497.18 g/mol（游离酸）

精确质量：497.00 g/mol（游离酸）

CAS号：327174-86-7（酸）

纯度：≥ 95%（高效液相色谱法）

形态：水溶液

颜色：无色至微黄

浓度：100 mM - 110 mM

pH值：7.5 ±0.5

光谱特性：最大吸收波长277 nm，摩尔吸光系数9.0 L mmol^-1 cm^-1（Tris-HCl pH 7.5）

相关产品：

高产T7 RNA合成试剂盒，#RNT-101

5-Methyl-CTP参考文献：

[1] Adams et al. (2014) 185 CELLULAR VIABILITY AND EXPRESSION OF GREEN FLUORESCENT PROTEIN IN BOVINE FETAL FIBROBLASTS FOLLOWING TRANSFECTION OF SYNTHETIC mRNA INCLUDING MODIFIED BASES. Reprod.Fertil. Dev. 26:207.

[2] Tchigvintsev et al. (2013) Biochemical and Structural Studies of Conserved Maf Proteins Revealed Nucleotide Pyrophosphatases with a Preference for Modified Nucleotides. Chem. Biol. Oxford U.K. 20:1386.

[3] Wong et al. (1997) A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids Res. 25:4169.

[4] Kormann et al. (2011) Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. Nature Biotechnology 29:154.

[5] Warren et al. (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7 (5):618.