Jena Bioscience/艾美捷6-硫代-GTP：在RNA合成与修饰中的机制研究

Jena Bioscience-6-硫代-GTP的应用：

对大肠杆菌 CTP 合酶的变构作用[1]

抑制 Vac-1-Rac 信号转导[2]

通过阻断 GTPase 激活进行免疫抑制[3, 4]

免疫抑制药物（移植、炎症）[1, 4]

艾美捷Jena Bioscience 6-硫代-GTP#NU-1106S参数：

适合一般实验室使用。

运输：通过凝胶包运输

储存条件：储存于 -20 °C

可以短期暴露于环境温度（累计最多 1 周）。

保质期：交货后12个月

分子式： C 10 H 16 N 5 O 13 P 3 S（游离酸）

分子量： 539.24 g/mol（游离酸）

精确质量： 538.97 g/mol（游离酸）

CAS#： 17670-19-8

纯度： ≥95%（HPLC）

形式：水溶液

颜色：无色至微黄色

浓度： 10 mM - 11 mM

pH值： 7.5±0.5

光谱性质： λ max  257 nm，ε 8.8 L mmol ^-1  cm ^-1 (Tris-HCl pH 7.5)，λ max  342 nm，ε 24.8 L mmol ^-1  cm ^-1 (Tris-HCl pH 7.5)

Jena Bioscience-6-硫代-GTP文献参考：

[1] Lunn et al. (2008) Structural requirements for the activation of Escherichia coli CTP synthase by the allosteric effector GTP are stringent, but requirements for inhibition are lax. J. Biol Chem. 283:2010.

[2] Wang et al. (2007) Enhanced cardiac allograft survival by Vav1-Rac signaling blockade in a mouse model. Transplant Immunology 18:53.

[3] Poppe et al. (2005) Azothioprine suppresses Ezrin-Radixin-Moesin-dependent T cell-APC conjugation through inhibition of Vav Guanosine exchange activity on Rac proteins. J. Immunology 176:640.

[4] Tiede et al. (2003) CD28-dependent Rac1 activation is the molecular target of azathioprine in primary human CD4+t lymphocytes. J. Clinical Investigation 111:1133.

Karner et al. (2010) Determination of 6-thioguanosine diphosphate and triphosphate and nucleoside diphosphate kinase activity in erythrocytes: novel targets for thiopurine therapy? Ther. Drug Monit. 32 (2):119.

Bao et al. (2008) Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent wG binding to a group I intron. Nucleic Acids Research 36 (21):6934.

Du et al. (2007) Enhanced cardiac allograft survival by Vav1-Rac signalling blockade in a mouse model. Transplant Immunology 18 (1): 53.

Pestova et al. (2006) Specific functional interactions of nucleotides at key -3 and +4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complex. Genes & Development 20:624.

Neurath et al. (2005) 6-Thioguanosine Diphosphate and Triphosphate Levels in Red Blood Cells and Response to Azathioprine Therapy in Crohn's Disease. Clinical Gastroenterology and Hepatology 3 (10): 1007.

Poland et al. (1997) Entrapment of 6-thiophosphoryl-IMP in the active site of crystalline adenylosuccinate synthetase from Escherichia coli. J. Biol. Chem. 272 (24):15200.

Yarbrough et al. (1985) Kinetics of interaction of 2-amino-6-mercapto-9-beta-ribofuranosylpurine 5'-triphosphate with bovine brain tubulin. Biochemistry-US 24 (7):1708.

Fishback et al. (1984) Interaction of 6-mercapto-GTP with bovine brain tubulin - equilibrium aspects. J. Biol. Chem. 259 (3):1968.

Jena Bioscience-6-硫代-GTP相关产品：

CGR-101, Silicone Grease

EN-109, BseCI

NU-1101, N6-Methyl-ATP

NU-1107, Guanosine-3',5'-bisphosphate

NU-1110, 6-Mercaptopurine-riboside-5'-triphosphate

NU-112, 8-Bromo-ADP

NU-118, 8-Bromo-GTP

NU-124, 8-Bromo-GDP

NU-201, Mant-ADP

NU-204, Mant-GDP

JenaBioscience的主要产品包括核苷/核酸制品系列（包括合成的类似物）、重组蛋白及蛋白表达系统、点击化学产品系列、在结构学研究方面全面提供晶体筛选试剂盒、优化试剂、工具和各类耗材等。此外，还提供分子生物学和生物化学研究的定制试剂与研究服务。艾美捷科技是Jena Bioscience的中国代理商，为科研工作者提供优质的产品与服务。