LDN-雌二醇ELISA（RUO）试剂盒检测原理

雌二醇ELISA是一种酶免疫测定法，用于定量测定血清或血浆（EDTA、肝素锂或柠檬酸盐血浆）中的雌二醇。

艾美捷LDN-雌二醇ELISA（RUO）试剂盒（FRE-2000R）：

名称：雌二醇ELISA

描述：

用于血清或血浆中雌二醇定量测量的酶免疫测定法。

设计：ELISA

样品体积：

25 ?l 血清或血浆

总测定时间：2小时

标准范围：0/25 – 2,000 pg/ml

灵敏度：10.6 pg/ml

储存：2 – 8 °C

试剂盒：96次测定

CE认证：是

LDN-雌二醇ELISA（RUO）试剂盒检测原理：

雌二醇ELISA试剂盒是一种固相酶联免疫吸附试验（ELISA），基于竞争性结合的原理。微孔板孔被涂覆有针对雌二醇分子抗原位点的多克隆[兔]抗体。

在第一次孵育过程中，添加的样本中的雌二醇与添加的酶联物竞争结合，后者是与辣根过氧化物酶结合的雌二醇，以结合到涂覆的抗体上。经过洗涤步骤去除所有未结合的物质后，将固相与底物溶液孵育。通过添加终止溶液来停止比色反应，测量所得黄色产物的光密度（OD）。颜色的强度与样本中分析物的浓度成反比。

通过绘制标准品浓度对OD值的标准曲线，使用该标准曲线确定未知样本的浓度。

LDN-雌二醇ELISA（RUO）试剂盒文献参考：

1. Tsang, B.K., Armstrong, D.T. and Whitfield, J.F., Steroid biosyntheses by isolated human ovarianfollicular cells in vitro, J. Clin. Endocrinol. Metab. 51:1407 - 11 (1980).

2. Gore-Langton, R.E. and Armstrong, D.T., Follicular stoidogenesis and its control. In: The physiology ofReproduction, Ed.: Knobil, E., and Neill, J. et al., pp. 331-85. Raven Press, New York (1988).

3. Hall, P.F., Testicular Steroid Synthesis: Organization and Regulation. In: The Physiology of Reproduction,Ed.: Knobil, E., and Neill, J. et al., pp 975-98. Raven Press, New York (1988).

4. Siiteri, P.K. Murai, J.T., Hammond, G.L., Nisker, J.A., Raymoure, W.J. and Kuhn, R.W., The serumtransport of steroid hormones, Rec. Prog. Horm. Res. 38:457 - 510 (1982).

5. Martin, B., Rotten, D., Jolivet, A. and Gautray, J-P-. Binding of steroids by proteins in follicular fluid of thehuman ovary, J.Clin. Endicrinol. Metab. 35: 443-47 (1981).

6. Baird, D.T., Ovarian steroid secretion and metabolism in women. In: The Endocrine Function of theHuman Ovary. Eds.: James, V.H:T., Serio, M. and Giusti, G. pp. 125-33,Academic Press, New York (1976).

7. McNastty, K.P., Baird, D.T., Bolton, a., Chambers, P., Corker, C.S. and McLean, H., concentration ofoestrogens and androgens in human ovarian venous plasma and follicular fluid throughout the menstrualcycle,J. Endocrinol. 71:77-85 (1976).

8. Abraham, G.E., Odell, W.D., Swerdloff, R.S., and Hopper, K., Simultaneous radioimmunoassay of plasmaFSH, LH, progesterone, 17-hydroxyprogesterone and estradiol-17? during the menstrual cycle,J.Clin. Endocrinol. Metab. 34:312-18 (1972).

9. March, C.M., Goebelsmann, U., Nakumara, R.M., and Mishell, D.R., Roles of oestradiol and progesteronein eliciting midcycle luteinising hormone and follicle stimulating hormone surges.J. clin. Endicrinol. Metab. 49:507-12 (1979).

10. Simpson, E.R., and McDonald, P.C., Endocrinology of Pregnancy. In: Textbook of Endocrinology,Ed.: Williams, R.H. pp412-22, Saunders Company, Philadelphia (1981).

11. Jenner, M.R., Kelch, R.P., et al., Hormonal Changes in prepubertal children, pubertal females and inprecocious puberty, premature thelarche, hypogonadism and in a child with feminising tumour,J. clin. Endocrinol. 34: 521 (1982).

12. Goldstein, D. et al., Correlation between oestradiol and progesterone in cycles with luteal phasedeficiency,Fertil. Steril. 37: 348-54 (1982).  

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