EpigenDx的甲基化基因组DNA对照。它们旨在作为DNA甲基化分析的阳性或阴性对照,可用于人类,小鼠,大鼠和猴子DNA甲基化分析测定。该高甲基化DNA是体外酶化基因组DNA,甲基化率大于85%;低甲基化DNA是化学和酶处理低于5%的甲基化。它们使用焦磷酸测序技术在许多基因特异性和全局甲基化分析中进行测试。
艾美捷甲基化基因组DNA对照控制数据:
图1:高甲基化对照gDNA(小鼠LINE1测定)
图2:低甲基化对照gDNA(人BRCA1测定)
甲基化基因组DNA对照详细信息:
高甲基化对照(人类/小鼠/大鼠/猴子)内容:
1瓶高甲基化基因组DNA(5微克,浓度为100纳克/微升,> 85% 甲基化)
低甲基化对照(人类/小鼠/大鼠/猴子)内容:
1瓶低甲基化基因组DNA(5微克,浓度为100纳克/微升,< 5% 甲基化)
预混合校准标准(人类/小鼠/大鼠/猴子)内容:
1瓶0% 甲基化基因组DNA(1微克,浓度为50纳克/微升)
1瓶5% 甲基化基因组DNA(1微克,浓度为50纳克/微升)
1瓶10% 甲基化基因组DNA(1微克,浓度为50纳克/微升)
1瓶25% 甲基化基因组DNA(1微克,浓度为50纳克/微升)
1瓶50% 甲基化基因组DNA(1微克,浓度为50纳克/微升)
1瓶75% 甲基化基因组DNA(1微克,浓度为50纳克/微升)
1瓶100% 甲基化基因组DNA(1微克,浓度为50纳克/微升)
甲基化基因组DNA对照描述:
Items Description
80-8061-HGHM5 Human high methylated genomic DNA (5 ?g at 100 ng/ ?L)
80-8062-HGUM5 Human low methylated genomic DNA (5 ?g at 100 ng/ ?L)
80-8063-MGHM5 Mouse high methylated genomic DNA (5 ?g at 100 ng/ ?L)
80-8064-MGUM5 Mouse low methylated genomic DNA (5 ?g at 100 ng/ ?L)
80-8065-RGHM5 Rat high methylated genomic DNA (5 ?g at 100 ng/ ?L)
80-8066-RGUM5 Rat low methylated genomic DNA (5 ?g at 100 ng/ ?L)
80-8067-PMGHM5 Monkey (Macaca) high methylated genomic DNA (5 ?g at 100 ng/ ?L)
80-8068-PMGUM5 Monkey (Macaca) low methylated genomic DNA (5 ?g at 100 ng/ ?L)
80-8060R-PreMix Rat Premixed Calibration Standard (1 ?g per vial, 20 ?L volume)
80-8060M-PreMix Mouse Premixed Calibration Standard (1 ?g per vial, 20 ?L volume)
80-8060H-PreMix Human Premixed Calibration Standard (1 ?g per vial, 20 ?L volume)
80-8060H-bsMix Human bisulfite modified genomic DNA controls including 15 ul of each low, medium and high methylated DNAs
80-8069-LHM5 High methylation controls for LUMA analysis
80-8070-LLM5 Low methylation controls for LUMA analysis
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