Biotin-11-dATP：非放射性DNA标记技术的核心

Botin修饰的脱氧腺苷5'-三磷酸被广泛用于各种非放射性DNA标记反应，包括缺口翻译、随机引物标记、cDNA标记和3'-末端标记。生物素化探针已被证明与同源核酸杂交的速度和程度与非生物素化的探针相同。杂交的生物素化DNA探针可以用抗生物素蛋白和链霉抗生物素素蛋白检测。Tris缓冲液（pH 7.5）中的生物素-11-dATP*1 mM*可以通过缺口翻译、随机引物、3'-末端标记或PCR过程酶促掺入DNA。数字“11”是指dATP和生物素之间连接体骨架中的碳原子数。生物素和抗生物素蛋白之间更有效的相互作用将发生在较长的连接体上，而较短的连接体将导致dATP更有效地掺入DNA。建议接头'11'长度对于大多数应用来说是最佳的。Tris缓冲液（pH 7.5）中的生物素-11-dATP*1 mM*在各种杂交应用中产生生物素化的DNA探针，包括Southern印迹、Northern印迹、斑点印迹、固定细胞和组织。其化学性质与PerkinElmer（PE）的NEL540001EA相当。

艾美捷Biotin-11-dATP：

货号：AAT-17014

规格：25纳摩尔

危险性说明短语：H303, H313, H333

危险符号：XN

预期用途：仅限研究使用（RUO）

风险说明短语：R20, R21, R22

储存：

冷冻（<-15°C）；尽量减少光照暴露

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