OX-34单克隆抗体与大鼠CD2反应,CD2是一种45-58kD的I型跨膜糖蛋白,也称为LFA-2、T11或Ly37。CD2是Ig超家族的成员。CD2由外周血淋巴细胞、胸腺细胞、形成E-玫瑰花结的所有T细胞和NK细胞的一个子集表达。CD2作为一种粘附受体,与CD58结合,导致CD2阳性T细胞和NK细胞的激活,并调节其细胞溶解活性。OX-34抗体已被证明可以阻断CD2与CD48的结合,并减轻大鼠已建立的佐剂性关节炎。
艾美捷InVivoMAb抗大鼠CD2/InVivoMAb anti-rat CD2:
货号:BE0417
同型:小鼠IgG2a,κ
推荐同型对照:InVivoMAb小鼠IgG2a同型对照,未知特异性
推荐稀释缓冲液:InVivoPure pH 7.0稀释缓冲液
偶联:未偶联。可通过BioXCell的抗体偶联服务进行偶联。
免疫原:大鼠T细胞母细胞
报告应用:体内CD2阻断
体外CD2阻断
西方印迹
配方:PBS,pH 7.0
不含稳定剂或防腐剂
内毒素:<2EU/mg(<0.002EU/μg)
由LAL凝胶凝集试验确定
纯度:>95%
由SDS-PAGE确定
无菌性:0.2微米过滤
生产:在无动物设施中从细胞培养上清液中纯化
纯化:蛋白G
分子量:150 kDa
储存:抗体溶液应以原液浓度在4°C下储存。不要冷冻。
InVivoMAb抗大鼠CD2/InVivoMAb anti-rat CD2文献参考:
in vivo CD2 blockade
Hoffmann JC, Herklotz C, Zeidler H, Bayer B, Westermann J. (1997). "Anti-CD2 (OX34) MoAb treatment of adjuvant arthritic rats: attenuation of established arthritis, selective depletion of CD4+ T cells, and CD2 down-modulation" Clin Exp Immunol 110(1):63-71.
in vivo CD2 blockade
Hoffmann JC, Herklotz C, Zeidler H, Bayer B, Rosenthal H, Westermann J. (1997). "Initiation and perpetuation of rat adjuvant arthritis is inhibited by the anti-CD2 monoclonal antibody (mAb) OX34" Ann Rheum Dis 56(12):716-22.
Western Blot
He Q, Beyers AD, Barclay AN, Williams AF. (1988). "A role in transmembrane signaling for the cytoplasmic domain of the CD2 T lymphocyte surface antigen" Cell 54(7):979-84.
in vitro CD2 blockade
Seaman WE, Eriksson E, Dobrow R, Imboden JB. (1987). "Inositol trisphosphate is generated by a rat natural killer cell tumor in response to target cells or to crosslinked monoclonal antibody OX-34: possible signaling role for the OX-34 determinant during activation by target cells" Proc Natl Acad Sci U S A 84(12):4239-43.
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