mCherry是一种红色荧光蛋白,它是从珊瑚Discosoma sp.中发现的荧光蛋白DsRed改良而来的。mCherry具有以下几个特点:
1. 亮度高:mCherry是一种非常亮的荧光蛋白,这使得它在显微镜下容易观察。
2. 成熟速度快:与DsRed相比,mCherry的成熟速度更快,这有助于减少在细胞内形成聚集体的可能性。
3. 光稳定性好:mCherry具有较好的光稳定性,适合长时间观察。
4. pH稳定性:mCherry对pH的敏感性较低,这使得它在不同的细胞环境中都能保持荧光特性。
5. 应用广泛:mCherry广泛应用于细胞生物学研究,包括蛋白质定位、细胞标记、活细胞成像等。
6. 光谱特性:mCherry的吸收峰大约在587-590纳米,发射峰在610-615纳米,这使得它适合与其他荧光蛋白(如GFP或YFP)进行多色标记和成像。
7. 遗传编码:mCherry可以通过基因工程手段融合到目标蛋白上,用于研究蛋白质的表达、定位和相互作用。
8. 转基因模型:mCherry也常被用于构建转基因动物模型,以研究特定基因的功能和调控。
mCherry的这些特性使其成为生物医学研究中一个非常有用的工具。通过将mCherry与其他蛋白质或肽段融合,研究人员可以观察和分析目标蛋白在细胞内的行为和功能。
Anti-mCherry,抗mCherry(Cherry荧光蛋白)山羊多克隆抗体,货号AB0040-200/AB0040-500,应用包括WB, IHC (F), IHC (P), IF, IEM。
Anti-mCherry,AB0040-200/AB0040-500应用示例:
免疫荧光--利用mccherry - rab11a转导的MCR细胞使用抗mCherry抗体处理;
用甲醇和抗rab11a抗体按1/250的比例固定细胞。
1/ 1000稀释的抗mCherry抗体;转染myc-mCherry的293细胞。
免疫荧光检测:在果蝇(Drosophila)幼虫的神经肌肉接头(NMJ)处,神经元表达mCherry荧光蛋白的转基因果蝇模型。使用1/ 1000稀释的抗mCherry 抗体(一抗)和1/500稀释的抗山羊IgY抗体偶联DyLight?488(二抗)。
免疫金标记RPE,体内注射mCherry表达载体。
用mcherry Rab1a腺病毒转导293 HEK细胞,使用抗mCherry抗体进行染色。
mcherry - EEA1转染的COS-7细胞使用抗mcherry抗体(1/100稀释)。
免疫荧光--用mCherry-Rab11a转导的hCEC细胞应用抗mcherry抗体;用甲醇和抗ra11a抗体按1/250的稀释比例固定细胞。
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