Nature同款，5hmC-羟化酶TET活性/抑制分析试剂盒

DNA胞嘧啶的表观遗传修饰通过调节基因转录在发育中起关键作用。目前研究最多的胞嘧啶修饰是5-甲基胞嘧啶（5mC），它与基因表达的抑制有关。在脱甲基化过程中，5mC氧化为连续的中间状态，包括5-羟甲基胞嘧啶（5hmC），5-甲酰基胞嘧啶（5fC）和5-羧基胞嘧啶（5caC）。在过去的十年中，研究表明，氧化形式的5mC，尤其是5hmC，除了在活性脱甲基过程中充当“中间体”外，还可能具有多种独立的功能。很多研究表明，5hmC 表达水 平与基因表达调控、多能干细胞分化、神经元发育、以及肿瘤发生都密切相关。

而参与其中甲基化循环的一个重要酶类，就是TET家族蛋白！人TET蛋白家族包含TET1、TET2和TET3这3个成员，所有TET蛋白都包含一个保守的双链β-螺旋结构域、一个富含半胱氨酸区和C端的核心催化结构域。TET1主要在胚胎干细胞中表达，TET2主要在造血系统中表达，TET3则在 小脑、皮质和海马体等神经系统中广泛表达。结果表明，基因组5hmC 水平与 TET 羟化酶活性相关。

那么如何检测5hmc羟化酶TET的活性呢？

作为专业的生命科学解决方案供应商，艾美捷为您推荐Nature同款方案：

Nature文章结果：

DOI https://doi.org/10.1038/s41590-020-0764-8

1.TET酶活性分析使用P-3086; c. 使用艾美捷推荐的P-1032检测整体5hmc水平

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Qian H et. al. (March 2021). TET1 promotes RXRα expression and adipogenesis through DNA demethylation.Biochim Biophys Acta Mol Cell Biol Lipids. :158919.

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Bhagat TD et. al. (October 2019). Lactate-mediated epigenetic reprogramming regulates formation of human pancreatic cancer-associated fibroblasts.8

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