山羊抗小鼠二抗是亲和纯化的抗体,对小鼠免疫球蛋白具有良好的特异性,可用于检测、分选或纯化其特定靶标。二抗提供了更高的通用性,使用户能够使用许多检测系统(如HRP、AP、荧光)。它们还可以通过信号放大提供更高的灵敏度,因为多个二抗可以与单个一抗结合。
AAT Bioquest-MegaWox polyHRP山羊抗小鼠IgG偶联物旨在在样本量有限或靶分子含量低的免疫测定中提供最高的灵敏度和低背景。纯化山羊抗小鼠IgG-聚-HRP偶联物,以去除与其HRP偶联物竞争结合位点的未偶联的山羊抗小鼠IgG分子。此外,共轭物中不含可引起背景信号的未共轭HRP。MegaWox?polyHRP山羊抗小鼠IgG与ELISA、蛋白质印迹、免疫组织化学(IHC)和核酸杂交检测中使用的显色、荧光和化学发光HRP底物相容。它已被验证可与AAT Bioquest的TSA和Styramide?荧光HRP底物一起使用,用于超灵敏检测低丰度生物靶标。
艾美捷AAT Bioquest-MegaWox polyHRP山羊抗小鼠IgG偶联物:
货号:11035
储存条件:2-6°C并避光保存。为了延长该产品的保质期,加入等体积的甘油使其最终浓度约为50%甘油,然后存放于-20°C。
有效期:自收到起12个月
浓度:1 mg/mL
配方:PBS,2 mg/mL BSA
物种反应性:小鼠
类别:二级
克隆性:多克隆
宿主:山羊
稳定剂:无
制备:山羊抗小鼠IgG (H+L) 是使用小鼠IgG池在山羊体内产生的,并通过与小鼠IgG偶联的珠子亲和纯化。该抗体在最佳条件下与MegaWox? polyHRP偶联。
应用:免疫细胞化学(ICC)、免疫组织化学(IHC)、西方印迹(WB)、酶联免疫吸附试验(ELISA)
可溶于:水
偶联物:MegaWox polyHRP
AAT Bioquest-MegaWox polyHRP山羊抗小鼠IgG偶联物文献参考:
Electrochemical POC device for fast malaria quantitative diagnosis in whole blood by using magnetic beads, Poly-HRP and microfluidic paper electrodes.
Authors: Ruiz-Vega, Gisela and Arias-Alpízar, Kevin and de la Serna, Erica and Borgheti-Cardoso, Livia Neves and Sulleiro, Elena and Molina, Israel and Fernàndez-Busquets, Xavier and Sánchez-Montalvá, Adrián and Del Campo, F Javier and Baldrich, Eva
Journal: Biosensors & bioelectronics (2020): 111925
Comparison of different methodologies and cryostat versus paraffin sections for chromogenic immunohistochemistry.
Authors: Hira, Vashendriya V V and de Jong, Annique Loncq and Ferro, Klea and Khurshed, Mohammed and Molenaar, Remco J and Van Noorden, Cornelis J F
Journal: Acta histochemica (2019): 125-134
The complementary role of insulin-like growth factor II mRNA-binding protein 3 (IMP3) in diagnosis of Hodgkin's lymphoma.
Authors: Masoud, Rokia and Ibrahiem, Afaf and Tantawy, Dina and Eldosoky, Ibrahim
Journal: Annals of diagnostic pathology (2019): 64-68
Detection of plasma MMP-9 within minutes. Unveiling some of the clues to develop fast and simple electrochemical magneto-immunosensors.
Authors: Ruiz-Vega, Gisela and García-Robaina, Alicia and Ben Ismail, Manel and Pasamar, Helena and García-Berrocoso, Teresa and Montaner, Joan and Zourob, Mohammed and Othmane, Ali and Del Campo, F Javier and Baldrich, Eva
Journal: Biosensors & bioelectronics (2018): 45-52
Using magnetic beads and signal amplifiers to produce short and simple immunoassays: Application to MMP-9 detection in plasma samples.
Authors: Ben Ismail, Manel and de la Serna, Erica and Ruiz-Vega, Gisela and García-Berrocoso, Teresa and Montaner, Joan and Zourob, Mohammed and Othmane, Ali and Baldrich, Eva
Journal: Analytica chimica acta (2018): 144-154
AAT Bioquest 致力于光谱学检测领域,包括吸收(颜色),荧光和发光技术。帮助科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。艾美捷科技是AAT Bioquest 的中国代理商,为科研工作者提供优质的产品与服务。
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