SouthernBiotech-DAPI Fluoromount-G是一种水溶性、含4',6-二脒基-2-苯基吲哚(DAPI)的化合物,推荐用于在具有水性最后步骤的染色程序后安装的载玻片。当与DNA结合时,DAPI会发出荧光,通常用作核/染色体复染。由于DAPI Fluoromount-G是水溶性的,可以通过将载玻片浸入PBS溶液中直到盖玻片松动来移除盖玻片。这种安装介质还为载玻片制剂的储存提供了半永久性密封。
艾美捷SouthernBiotech-Dapi-Fluoromount-G:
货号:0100-20
缓冲液配方:含有甘油、<0.1% 叠氮化钠和专有成分的磷酸盐缓冲液
体积:20毫升
推荐储存:储存在22-25°C
应用:免疫组化-冰冻切片、免疫组化-石蜡切片、免疫细胞化学、原位杂交、TUNEL 、邻近连接测定、迁移测定、凋亡
SouthernBiotech-Dapi-Fluoromount-G水溶性好,并且偶联Dapi,可在实验最后封片时使用。封片时使用该荧光封片剂不仅可以减少荧光淬灭,还可同时将细胞核染成蓝色,便于更好的分析实验结果。
SouthernBiotech-Dapi-Fluoromount-G文献参考:
1. Hagel C, Krasemann S, L?ffler J, Püschel K, Magnus T, Glatzel M. Upregulation of Shiga toxin receptor CD77/Gb3 and interleukin-1β expression in the brain of EHEC patients with hemolytic uremic syndrome and neurologic symptoms. Brain Pathol. 2015;25:146-56. (IHC-FS, AF555, FITC)
2. Cubbon RM, Yuldasheva NY, Viswambharan H, Mercer BN, Baliga V, Stephen S, et alL. Restoring Akt1 activity in outgrowth endothelial cells from South Asian men rescues vascular reparative potential. Stem Cells. 2014;32:2714-23. (IHC-FS, Chromeo? 642, CellTracker? CM-DiI)
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4. Weinger JG, Plaisted WC, Maciejewski SM, Lanier LL, Walsh CM, Lane TE. Activating receptor NKG2D targets RAE-1-expressing allogeneic neural precursor cells in a viral model of multiple sclerosis. Stem Cells. 2014;32:2690-701. (IHC-FS, AF594, EGFP)
5. Lee Y, Yoon HY, Shin JM, Saravanakumar G, Noh KH, Song K, et al. A polymeric conjugate foreignizing tumor cells for targeted immunotherapy in vivo. J Control Release. 2015;199:98-105. (IHC-FS, CY5.5)
6. Zhu M, Bakhru P, Conley B, Nelson JS, Free M, Martin A, et al. Sex bias in CNS autoimmune disease mediated by androgen control of autoimmune regulator. Nat Commun. 2016;7:11350. (IHC-FS, FITC, PE)
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