SouthernBiotech正常山羊血清,用作基于抗体的应用中的对照或阻断,包括流式细胞术、免疫组织化学和免疫细胞化学。
艾美捷SouthernBiotech-正常山羊血清:
货号:0060-01
来源:成年混合品种山羊
纯化方法:加工以最小化溶血,并进行0.2微米过滤
缓冲液配方:含有 < 0.1% 叠氮化钠
体积:10毫升
推荐储存条件:2-8°C
应用:
封闭
对照
流式细胞术
石蜡切片免疫组织化学
冰冻切片免疫组织化学
整体免疫组织化学
免疫细胞化学
人胰腺癌细胞系MIA PaCa-2,用10%正常山羊血清(SB目录号0060-01)封闭,并用小鼠抗细胞角蛋白18-UNLB(SB Cat。编号:10085-01;右图),接着是山羊抗小鼠IgG(H+L)
SouthernBiotech-正常山羊血清文献参考:
1. Savage AK, Constantinides MG, Han J, Picard D, Martin E, Li B, et al. The transcription factor PLZF directs the effector program of the NKT cell lineage. Immunity. 2008;29:391-403. (Block, FC)
2. Yu KO, Porcelli SA, Shuman HA. In vitro derivation of macrophage from guinea pig bone marrow with human M-CSF. J Immunol Methods. 2013;389:88-94. (Block, FC)
3. Falahat R, Wiranowska M, Gallant ND, Toomey R, Hill R, Alcantar N. A Cell ELISA for the quantification of MUC1 mucin (CD227) expressed by cancer cells of epithelial and neuroectodermal origin. Cell Immunol. 2015;298:96-103. (Block, FC, ELISA)
4. Johnson DT, Luong R, Lee SH, Peng Y, Shaltouki A, Lee JT, et al. Deletion of leucine zipper tumor suppressor 2 (Lzts2) increases susceptibility to tumor development. J Biol Chem. 2013;288:3727-38. (Block, IHC-PS, ICC)
5. Schopperle WM, inventor; CureMeta LLC, assignee. Systems and methods for employing podocalyxin and tra human stem cell markers as prognostic markers for aggressive and metastatic cancer. United States patent application publication US 2014/0274773 A1. 2014 Sep 18. (Block, IHC-PS)
6. Knuth CA, Witte-Bouma J, Ridwan Y, Wolvius EB, Farrell E. Mesenchymal stem cell-mediated endochondral ossification utilising micropellets and brief chondrogenic priming. Eur Cell Mater. 2017;34:142-61. (Block, IHC-PS)
7. Mitchell AC, Javidfar B, Bicks LK, Neve R, Garbett K, Lander SS, et al. Longitudinal assessment of neuronal 3D genomes in mouse prefrontal cortex. Nat Commun. 2016;7:12743. (Block, IHC-FS)
8. Szabadfi K, Atlasz T, Kiss P, Reglodi D, Szabo A, Kovacs K, et al. Protective effects of the neuropeptide PACAP in diabetic retinopathy. Cell Tissue Res. 2012;348:37-46. (Block, IHC-WM)
9. L?mmermann T, Afonso PV, Angermann BR, Wang JM, Kastenmüller W, Parent CA, et al. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature. 2013;498:371-5. (Block, IHC-WM)
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