worthington 垂体组织分离指南

发布者：艾美捷科技发布时间：2022-06-09

分享到：

原代细胞分离中，针对垂体组织涉及到垂体，滤泡，生长激素、垂体前叶和结节等等的解离通常会使用酶，如何选择合适的酶来完成对应垂体组织的解离，以及原代细胞采集的常规操作， 艾美捷针对垂体相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：垂体组织分离指南.png

本技术指南描述了标准实验室程序；为建立细胞分离方案提供了一种合乎逻辑的实验方法；并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。

垂体
种属	种属详情	细胞名称	酶组合推荐，含推荐浓度	培养基	参考文献
牛	Calf, male, less then 6 months old	垂体 / Pituitary	Collagenase: 0.3%	DMEM	Hassan, H., and Merkel, R.: Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells, In Vitro Cell Dev Biol 30A, 435, 1994
牛	Bovine	垂体 / Pituitary	Collagenase: 0.1%	EBSS, CMF	Mason, W. and Ingram, C.: Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells, Vol. 124,, , 207, 1986
牛	Bovine	滤泡，垂体前叶和结节 / Follicular, anterior pituitary and pars tuberalis	Deoxyribonuclease I: 200 /ml	HBSS, CMF	Ferrara, N., Goldsmith, P., Fujii, D., and Weiner, R.: Culture and Characterization of Follicular Cells of the Bovine Anterior Pituitary and Pars Tuberalis, Vol. 124,, , 245, 1986
牛	Calf, male, 1-6 week old	垂体 / Pitituary	Hyaluronidase: 0.1%	DMEM	Ridgway, E., Klibanski, A., Marorana, M., Milbury, P., Kieffer, J., and Chin, W.: The Effect of Somatostatin on the Release of Thyrotropin and its Subunits from Bovine Anterior Pitituary Cells in Vitro, Endocrinology 112 (6), 1937, 1983
小鼠	Mouse	垂体 / Pituitary	Collagenase Type 2: 0.5%； Deoxyribonuclease I: 0.005%	HBSS	Perez Millain, M., Brinkmeier, M., Mortensen, A. and Camper, S.: PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells., Elife 5, , 2016
小鼠	Mouse	垂体 / Pituitary	Collagenase Type 1: 1,000 u/ml； Soybean Trypsin Inhibitor: 0.01%； Hyaluronidase: 0.1%； Deoxyribonuclease I: 0.001%	DMEM	Pyczek, J., Buslei, R., Schult, D., Holsken, A., Buchfelder, M., Hess, I., Hahn, H. and Uhmann, A.: Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland., Sci Rep 6, 24928, 2016
小鼠	Mouse, male	垂体 / Pituitary	Collagenase: 0.4%； Hyaluronidase: 0.1%； Trypsin: 0.3%	DMEM/Han's F12	Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A: Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase, Endocrinology 144, 188-200, 2003
绵羊	Ovine, adult	生长激素 / Somatotropes	Collagenase Type 1: 0.3%； Hyaluronidase:	Medium 199	Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen: Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes, Endocrinology 143, 4609-19, 2002
大鼠	Rat, male, 12-15 week	垂体 / Pituitary	Collagenase Type 2: 0.4%； Deoxyribonuclease I: 0.04%	DMEM	Akieda-Asai, S., Zaima, N., Ikegami, K., Kahyo, T, Yao, I., Hatanaka, T., Iemura, S., Sugiyama, R., Yokozeki, T., Eishi, Y., Koike, M., Ikeda, K., Chiba, T., Yamaza, H., Shimokawa, I., Song, S., Matsuno, A., Mizutani, A., Sawabe, M. Chao, N., Tanaka, M., : SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals., PLoS ONE 5, e11755, 2010
大鼠	Rat, Wistar, male, 2 month old	垂体 / Pituitary	Trypsin: 0.1%	DMEM	Zhou, X., De Schepper, J., De Craemer, D., Delhase, M., Gys, G., Smitz, J., and Hooghe-Peters, E.: Pituitary Growth Hormone Release and Gene Expression in Cafeteria-Diet-Induced Obese Rats, J Endocrinol 159, 165, 1998
大鼠	Rat, SD, female, 200 - 250 g	垂体前叶 / Anterior pituitary gland	Trypsin: 0.1%	EBSS, CMF	D'Emden, M. and Wark, J.: Culture Requirements for Optimal Expression of 1,25-Dihydroxyvitamin D3-Enhanced Thyrotropin Secretion, In Vitro Cell Dev Biol 27, 197, 1991
大鼠	Rat	垂体 / Pituitary	Trypsin: 0.3 %	HEPES	Wilfinger, W., Larsen, W., Downs, T., and Wilbur, D.: An In Vitro Model for Studies of Intercellular Communication in Cultured Rat Anterior Pituitary Cells, Tissue Cell 16 (4), 483, 1984
大鼠	Rat, SD, male, 250-450 g	垂体前叶 / Anterior pituitary	Trypsin: 0.25%	Krebs	Portanova, R., Smith, D., and Sayers, G.: A Trypsin for the Preparation of Isolated Rat Anterior Pituitary, Proc Soc Exp Biol Med 133, 573, 1970