worthington 干细胞组织分离指南

发布者：艾美捷科技发布时间：2022-06-09

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原代细胞分离中，针对干细胞涉及到基质细胞血管，羊膜间充质干细胞、脂肪干细胞、产后牙周韧带，滑膜间充质干细胞等等的解离通常会使用酶，如何选择合适的酶来完成对应干细胞的解离，以及原代细胞采集的常规操作， 艾美捷针对干细胞相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：干细胞组织分离指南.png

本技术指南描述了标准实验室程序；为建立细胞分离方案提供了一种合乎逻辑的实验方法；并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。

干细胞
种属	种属详情	细胞名称	酶组合推荐，含推荐浓度	培养基	参考文献
犬/狗	23-33 kg	基质细胞血管 / Stromal vascular	Collagenase: see reference	Media 199E	Williams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts., Tissue Eng Part A 19, 1295-302, 2013
犬/狗	Canine	羊膜间充质干细胞 / Amniotic membrane MSC	Trypsin: 0.25%； Collagenase Type 1: 0.2%	LG-DMEM	Park, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells., PLoS ONE 7, e44693, 2012
犬/狗	Canine, 20-25 kg	脂肪干细胞 / Adipose stem cells	Collagenase: see reference	Media-199	Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force., J Surg Res 152, 157, 2009
马	Equine	脂肪干细胞 / Adipose derived stem cells	Collagenase Type 1: 0.1%	PBS	Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow., Vet Surg 37, 713, 2008
马	Equine, 1-5 year	脂肪干细胞 / Adipose derived stem cells	Collagenase Type 1: 0.1%	PBS	Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells., Vet Surg 36, 613, 2007
人	Human	脂肪来源的干细胞 / Adipose derived stem	Collagenase Type 4: 150-300 u/ml	RPMI-1640	Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat., Curr Protoc Stem Cell Biol 48, e68, 2019
人	Human	产后牙周韧带 / Postnatal periodontal ligament	Collagenase Type 1: 0.01%	DMEM/F12	Yam, G., Teo, E., Setiawan, M., Lovatt, M., Yusoff, N., Fuest, M., Goh, B. and Mehta, J.: Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy., J Cell Mol Med , , 2018
人	Human	沃顿果冻间充质基质细胞 / Wharton jelly mesenchymal stromal	Collagenase Type 2: 270 u/ml	DMEM/F12	Oppliger, B., Joerger-Messerli, M., Simillion, C., Mueller, M., Surbek, D. and Schoeberlein, A.: Mesenchymal Stromal Cells from Umbilical Cord Wharton's Jelly Trigger Oligodendroglial Differentiation in Neural Progenitor Cells Through Cell-to-Cell Contact., Cytotherapy 19, 829-838, 2017
人	Human	脐带间充质基质细胞 / Umbilical cord mesenchymal stromal	Collagenase Type 1: 0.2%； Hyaluronidase: 0.1%； Deoxyribonuclease I: 0.01%	MEM	de Witte, S., Lambert, E., Merino, A., Strini, T., Douben, H., O'Flynn, L., Elliman, S., de Klein, A., Newsome, P., Baan, C. and Hoogduijn, M.: Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion., Cytotherapy 19, 798-807, 2017
人	Human	滑膜间充质干细胞 / Synovial mesenchymal stem	Collagenase: 0.3%	MEM	Matsumura, E., Tsuji, K., Komori, K., Koga, H., Sekiya, I., Muneta, T.: Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells., Cytotherapy 19, 181-193, 2017
人	Human	脂肪来源的间充质干细胞 / Adipose derived mesenchymal stem	Collagenase animal free: 0.1%	PBS	Clark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome., Stem Cell Res Ther 8, 69, 2017
人	Human	运动神经元 / Motor neurons	Papain: 20 u/ml	EBSS	Rigamonti, A., Repetti, G., Sun, C., Price, F., Reny, D., Rapino, F., Weisinger, K., Benkler, C., Peterson, Q., Davidow, L., Hansson, E. and Rubin, L.: Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System., Stem Cell Reports 6, 993-1008, 2016
人	Human	沃顿果冻间充质基质细胞 / Wharton jelly mesenchymal stromal	Collagenase Type 2: 0.6%	DMEM	Lim, J., Razi, Z., Law, J., Nawi, A., Idrus, R. and Ng, M.: MSCs can be Differentially Isolated From Maternal, Middle and Fetal Segments of the Human Umbilical Cord., Cytotherapy 18, 1493-1502, 2016
人	Human	脂肪干细胞 / Adipose stem	Collagenase Type 1: 0.1%	DMEM/F12	Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells, Cytotherapy 18, 371-83, 2016
人	Human	腱干细胞 / Tendon stem	Collagenase Type 1: 0.3%； Neutral Protease: 0.4%	MEM	Randelli, P., Menon, A., Ragone, V., Creo P., Alfieri, M., Perucca, O., Banfi, G., Cabitza, P., Tettamanti, G. and Anastasia, L.: Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study., BMC Complement Altern Med 16, 293, 2016
人	Human	间充质基质细胞 / Mesenchymal stromal	Collagenase Type 4: 0.2%	PBS	Choudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells., Cytotherapy 17, 359-68, 2015
人	Human	脂肪干细胞 / Adipose derived stem cells	Collagenase Type 2: 0.1%	HBSS	Satish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage., BMC Med Genomics 8, 41, 2015
人	Human	牙间充质干细胞 / Dental mesenchymal stem	Collagenase Type 1: 0.3%； Neutral Protease: 0.4%	DMEM	Wu, Z., Wang, J., Dong, R., Wang, L., Fan, Z., Liu, D. and Wang, S.: Depletion of MEIS2 Inhibits Osteogenic Differentiation Potential of Human Dental Stem Cells., Int J Clin Exp Med 8, 7220-30, 2015
人	Human	蜕膜间充质干细胞 / Decidua mesenchymal stem	Trypsin: 0.25%； Collagenase Type 1: 1.0%； Deoxyribonuclease I: 0.005%	PBS	Kusuma, G., Menicanin, D., Gronthos, S., Manuelpillai, U., Abumaree, M., Pertile, M., Brennecke, S. and Kalionis, B.: Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua., PLoS ONE 10, e0141246, 2015
人	Human	骨骼肌干细胞 / Skeletal muscle stem	Collagenase Type 2: 750 u/ml； Neutral Protease: 2 u/ml	Ham's F10	Charville, G., Cheung, T., Yoo, B., Santos, P., Lee, G., Shrager, J. and Rando, T.: Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells., Stem Cell Reports 5, 621, 2015
人	Human	脊髓神经祖细胞 / Spinal cord neural progenitor	PDS kit: with modifications	EBSS	Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp , e52732, 2015
人	Human	华顿果冻间充质干细胞 / Wharton jelly mesenchymal stem	Collagenase Type 2: 0.1%； Trypsin NF 1:250: 0.25%	PBS	Lee, S., Park, B., Kim, J., Jekarl, D., Choi, H., Lee, S., Kim, M., Kim, Y. and Park, M.: The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells., Cytotherapy 17, 1723-31, 2015
人	Human, adult	脂肪来源的间充质干细胞 / Adipose derived mesenchymal stem	Collagenase Type 2: 0.1%	see reference	Al-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells., Cytotherapy 16, 915, 2014
人	Human, adult	脂肪来源的干细胞 / Adipose derived stem	Collagenase Type 1: 0.15%	DMEM	Koellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities., Cytotherapy 16, 789, 2014
人	Human	脐间充质干细胞 / Umbilical mesenchymal stem	Collagenase Type 2: 0.1%； Trypsin: 0.25%	DMEM	Hang, H., Yu, Y., Wu, N., Huang, Q., Xia, Q. and Bian, J.: Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction., PLoS ONE 9, e104133, 2014