worthington 腮腺组织分离指南

发布者：艾美捷科技发布时间：2022-06-09

分享到：

原代细胞分离中，针对腮腺组织涉及到腮腺和舌下腺，腺泡、腮腺腺泡细胞、上皮，腺泡，眶外泪腺，腮腺，胰腺等等的解离通常会使用酶，如何选择合适的酶来完成对应腮腺组织的解离，以及原代细胞采集的常规操作， 艾美捷针对腮腺相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：组织分离指南.png

本技术指南描述了标准实验室程序；为建立细胞分离方案提供了一种合乎逻辑的实验方法；并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。

腮腺
种属	种属详情	细胞名称	酶组合推荐，含推荐浓度	培养基	参考文献
小鼠	Mouse	腮腺和舌下腺 / Parotid and sublingual glandular	Neutral Protease: 50 u/ml； Collagenase Type 1: 100 u/ml	PBS	Ogawa, M., Oshima, M., Imamura, A., Sekine, Y., Ishida, K., Yamashita, K., Nakajima, K., Hirayama, M., Tachikawa, T. and Tsuji, T.: Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ., Nat Commun 4, 2498, 2013
大鼠	Rat, Wistar, male, 130 g	腺泡 / Acinar	Collagenase: 75 u/ml； Hyaluronidase: 153 u/ml	RPMI 1640	Looms, D., Dissing, S., Tritsaris, K., Pedersen, A. and Nauntofte, B.: Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells., Adv Dent Res 14, 62, 2000
大鼠	Rat, SD, 100-120 g	腮腺腺泡细胞 / Parotid acinar cells	Collagenase Type 2: 90 u/ml	Krebs-Henseleit Bicarbonate	D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL: Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes, J Histochem Cytochem 45, 965-73, 1997
大鼠	Rat, SD, male	上皮 / Epithelial	Collagenase: 50-75 u/ml； Hyaluronidase: 0.1%	HBSS CF	Prasad, K.N., Edwards-Prasad, J., Carvaiho, I., LaRosa, F.G., Baibinder, E., Meyers, A., and Quissell, D.: Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments, In Vitro Cell Dev Biol 28, 493, 1992
大鼠	Rat, SD, male, 100-150 g	腮腺腺泡 / Parotid acinar	Trypsin: 0.001%	F12 medium	Yeh, C., Mertz, P., Oliver, C., Baum, B., and Kousvelari, E.: Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells, In Vitro Cell Dev Biol 27, 707, 1991
大鼠	Rat, Wistar, male	腮腺腺泡 / Parotid acinar	Trypsin: 0.02%	Solution B (See reference)	Foskett, J., Roifman, C. and Wong, D.: Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells, J Biol Chem 266, 2778, 1991
大鼠	Rat	腮腺 / Parotid	Hyaluronidase: 0.025%	HBSS with 20mM HEPES	Takuma, T. and Ichida, T.: Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP, J Invest Dermatol 103, 95, 1988
大鼠	Rat, Wistar, male, 250 - 350 g	腺泡，腮腺 / Acinar, parotid	Hyaluronidase: 0.015%	Earle's MEM	Melvin, J., Kawaguchi, M., Baum, B., and Turner, R.: A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells, Biochem Biophys Res Commun 145, 754, 1987
大鼠	Rat, SD, male, 50 - 125 g	腺泡，眶外泪腺，腮腺，胰腺 / Acinar，Exorbital lacrimal, parotid, pancreas	Trypsin: 0.01%	HBSS, CMF	Oliver, C., Waters, J., Tolbert, C., and Kleinman, H.: Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel, In Vitro Cell Dev Biol 23, 465, 1987
大鼠	Rat, SD, albino, male, 150-200 g	腺泡，腮腺 / Acinar, parotid	Trypsin: 0.01%； Collagenase: 40-50 u/ml； Hyaluronidase: 0.10%	HBSS CMF	Mangos, J., McSherry, N., Butcher, F., Irwin, K., and Barber, T.: Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character), Am J Physiol 229 (3), 560, 1975