worthington 其他组织分离指南

发布者：艾美捷科技发布时间：2022-06-07

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原代细胞分离中，针对其他组织涉及到髓核，肌腱细胞、牙髓干细胞、口腔上皮等等的解离通常会使用酶，如何选择合适的酶来完成对应其他组织的解离，以及原代细胞采集的常规操作， 艾美捷针对其他相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：其它组织分离指南

本技术指南描述了标准实验室程序；为建立细胞分离方案提供了一种合乎逻辑的实验方法；并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。

其他组织
种属	种属详情	细胞名称	酶组合推荐，含推荐浓度	培养基	参考文献
牛	Bovine	髓核 / Nucleus pulposus	Collagenase Type 2: 0.2%	DMEM	Lee, J., Cheung, K. and Leung, V.: Systematic Study of Cell Isolation from Bovine Nucleus Pulposus: Improving Cell Yield and Experiment Reliability., J Orthop Res 33, 1743-55, 2015
马	Equine	肌腱细胞 / Tendon Cells	Collagenase Type 1: 0.1%	DMEM	Nemoto, M., Kizaki, K., Yamamoto, Y., Oonuma, T. and Hashizume, K.: Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration., J Equine Sci 24, 17-24, 2013
山羊	Goat	髌下脂肪垫间充质基质细胞 / Infrapatellar fat pad mesenchymal stromal	Collagenase: 0.15%	DMEM	Arora, A., Sriram, M., Kothari, A. and Katti, D.: Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering., Cytotherapy 19, 881-894, 2017
人	Human	粘附成纤维细胞 / Adhesion fibroblasts	Collagenase Type 4: 0.2%	Medium 199	Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation., Nat Commun 11, 4061, 2020
人	Human	髓核 / Nucleus pulposus	Pronase: 0.25%； Collagenase Type 2: 600 u/ml	DMEM	Sun, Y., Lv, M., Zhou, L., Tam, V., Lv, F., Chan, D., Wang, H., Zheng, Z., Cheung, K. and Leung, V.: Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation., Osteoarthritis Cartilage 23, 1194-203, 2015
人	Human, 3-7 year	牙髓干细胞 / Dental pulp stem cells	Collagenase Type 1: 0.3%； Neutral Protease: 0.4%	DMEM	Salmon, B., Bardet, C, Khaddam, M. Naji, J., Coyac, B., Baroukh, B., Letourneur, F., Lesieur, J., Decup, F., Le Denmat, D., Nicoletti, A., Poliard, A, Rowe, P., Huet, E., Vital, S. and Linglart, A.: MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia., PLoS ONE 8, e56749, 2013
人	Human	牙髓干细胞 / Dental pulp stem	Collagenase Type 1: 0.3%； Neutral Protease: 0.4%	DMEM/F12	Bonnamain, V., Thinard, R., Sergent-Tanguy, S., Huet, P., Bienvenu, G., Naveilhan, P., Farges, J. and Alliot-Licht, B.: Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium., Front Physiol 4, 357, 2013
人	Human	间充质基质细胞 / Mesenchymal stromal	Collagenase Type 1: 0.075%	PBS	Lee, Y., Lee, J., Park, H., Lim, Y., Lee, J., Wang, S. and Lee, B.: Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adenoid Tissue., Cell Physiol Biochem 31, 513-24, 2013
人	Human	菌状味乳头 / Fungiform taste papillae	Collagenase Type 1: 550 u/ml； Elastase: 10 u/ml； Soybean Trypsin Inhibitor: 0.09%	see reference	Ozdener, H., Spielman, A. and Rawson, N.: Isolation and Culture of Human Fungiform Taste Papillae Cells, J Vis Exp 63, e3730, 2012
人	Human	肌腱细胞 / Tendon cells	Collagenase Type 2: 0,3%	DMEM/Hams F12	Pauly, S., Klatte, F., Strobel, C., Schmidmaier, G., Greiner, S. and Scheibel, M. Wildemann B: Characterization of Tendon Cell Cultures of the Human Rotator Cuff., Eur Cell Mater 20, 84-97, 2010
人	Human	脂肪来源的基质细胞 / Adipose derived stromal	Collagenase Type 2: 0.075%	DMEM	Panetta, N., Gupta, D., Kwan, M., Wan, D., Commons, G. and Longaker, M.: Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells., Plast Reconstr Surg 124, 65, 2009
人	Human	口腔上皮 / Oral epithelial	Neutral Protease: 1.2 u/ml； Trypsin: 0.05%	PBS	Nakamura, T., Endo, K. and Kinoshita, S.: Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling., Stem Cells 25, 628-38, 2007
人	Human	颈动脉斑块巨噬细胞 / Carotid artery plaque macrophage	Collagenase Type 4: 450 u/ml； Deoxyribonuclease I: 500 u/ml； Soybean Trypsin Inhibitor: 0.1%	HBSS	Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM: Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin, Circ Res 98, 1282-9, 2006
人	Human	食管微血管内皮 / Esophageal microvascular endothelial	Collagenase Type 2: 0.2%	MCDB-131	Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation., Am J Physiol Gastrointest Liver Physiol 285, G1277, 2003
人		胃上皮细胞 / Gastric epithelial cells	Collagenase Type 2: 200 u/ml； Neutral Protease: 1.2 u/ml； Soybean Trypsin Inhibitor: 0.125%	L-15	Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22, 133, 2000
人	Human	滑膜细胞 / Synoviocytes	Collagenase: 0.2%	DMEM/F12	Chen V, Croft D, Purkis P, Kramer IM: Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release., Br J Rheumatol 37, 148-56, 1998
人	Human	消化细胞 / Peptic cells	Collagenase Type 4: 0.1%； Soybean Trypsin Inhibitor: 0.2%	Ringer solution	Lanas. A., Nerin, J., Esteva, F. and Sainz, R.: Non-Steroidal Anti-Inflammatory Drugs and Prostaglandin Effects on Pepsinogen Secretion by Dispersed Human Peptic Cells., Gut 36, 657-63, 1995
人	Human	根尖肉芽肿 / Periapical granuloma	CLSPA: 0.25%	RPMI-1640	Stern MH, Dreizen S, Mackler BF, Levy BM: Isolation and characterization of inflammatory cells from the human periapical granuloma, J Dent Res 61, 1408-12, 1982
昆虫	Insect, Lepidoptera	鳞翅目 / Lepitopteran	Collagenase Type 3: 0.35%； Hyaluronidase: 0.01%	Dulbecco PBS	Goodwin, R and McCawley, P: Initiating Attached Cell Lines From the Lepidoptera (Insecta), Meth Cell Sci 3, 567, 1977
无脊椎动物	Squid	地幔 / Mantle	Collagenase: see reference； Papain: see reference	Phosphate buffer	Raman, M. and Mathew, S.: Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny., J Food Sci Technol 51, 1509-16, 2014
小鼠	Mouse	类器官培养物 / Organoid cultures	Stemxyme 1: 0.1%	PBS	Huang, L., Desai, R., Conrad, D., Leite, N., Akshinthala, D., Lim, C., Gonzalez, R., Muthuswamy, L., Gartner, Z. and Muthuswamy, S.: Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids.,Cell Stem Cell Vol. 28,, , 1090-1104.e6, 2021
小鼠	Mouse, embryonic	气管 / Trachea	Elastase: 0.1%； Neutral Protease: 0.25%； Deoxyribonuclease I: 0.01%； Trypsin: 0.125%	DMEM/F12	He, M., Wu, B., Ye, W., Le, D., Sinclair, A., Padovano, V., Chen, Y., Li, K., Sit, R., Tan, M., Caplan, M., Neff, N., Jan, Y., Darmanis, S. and Jan, L.: Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway., Elife 9, 53085, 2020
小鼠	Mouse	粘附成纤维细胞 / Adhesion fibroblasts	Collagenase Type 4: 0.2%	Medium 199	Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation., Nat Commun 11, 4061, 2020
小鼠	Mouse, 8-12 week	膀胱 / Urinary bladder	Collagenase Type 6: 20 u/mlCollagenase Type 1: 50 u/ml； Collagenase Type 3: 40 u/ml； Papain: 45 u/ml； Deoxyribonuclease I: 4 u/ml；	Tyrode solution	Atta, H., Gheinani, A., Wacker, A., Heshmati, Y., Bigger-Allen, A., Lambrinos, G., Gao, Y., Bielenberg, D. and Adam, R.: A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury., J Vis Exp , , 2020
小鼠	Mouse	尿路上皮 / Urothelial	Collagenase Type 1: 0.5%	PBS	Huang, C., Chen, C. and Shyr, C.: The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer., Cytotherapy 19, 1233-1245, 2017
小鼠	Mouse	骨髓基质细胞 / Bone marrow stromal	Collagenase Type 1: 0.2%	PBS	Ishida, T., Suzuki, S., Lai, C., Yamazaki, S., Kakuta, S., Iwakura, Y., Nojima, M., Takeuchi, Y., Higashihara, M., Nakauchi, H. and Otsu, M.: Pre-Transplantation Blockade of TNF-α-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells., Stem Cells 35, 989-1002, 2017
小鼠	Mouse	耳蜗 / Cochlear	Papain: 20 u/ml； Trypsin: 0.05%	DMEM	Kim, Y., Wang, S., Tymanskyj, S., Ma, L., Tao, H. and Zhang, L.: Dcc Mediates Functional Assembly of Peripheral Auditory Circuits., Sci Rep 6, 23799, 2016
小鼠	Mouse	上皮干细胞 / Epithelial stem	Collagenase: 2%	DMEM/F12	Chavez, M., Hu, J., Seidel, K., Li, C., Jheon, A., Naveau, A., Horst, O. and Klein, O.: Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor., J Vis Exp , , 2014
小鼠	Mouse, 26-30 g	肌间丛 / Myenteric plexus	Collagenase Type 2: 0.13%	Neurobasal A	Smith, T., Grider, J., Dewey, W. and Akbarali, H.: Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels., PLoS ONE 7, e45251, 2012
小鼠	Mouse, 10 week	滑膜间充质 / Synovial mesenchymal	Collagenase: 0.1%； Deoxyribonuclease I: 0.005%	DMEM	Futami, I., Ishijima, M., Kaneko, H., Tsuji, K., Ichikawa-Tomikawa, N., Sadatsuki, R., Muneta, T., Arikawa-Hirasawa, E., Sekiya, I. and Kaneko, K.: Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium., PLoS ONE 7, e45517, 2012