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worthington 内皮细胞组织分离指南

发布者:艾美捷科技    发布时间:2022-06-01     
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原代细胞分离中,针对脑组织涉及到微血管内皮、脑内皮细胞、神经元和小胶质细胞、微血管等等的解离通常会使用酶,如何选择合适的酶来完成对应脑组织的解离,以及原代细胞采集的常规操作, 艾美捷针对脑相关的组织分类解离分种属、不同的细胞、酶组合推荐,推荐浓度、培养基以及参考文献,详细汇总指南:

内皮组织分离指南

本技术指南描述了标准实验室程序; 为建立细胞分离方案提供了一种合乎逻辑的实验方法; 并列出了许多组织特异性参考资料,以帮助开发有效的方法。更多其他组织的解离指南,点击查看:各组织分离指南

 

内皮细胞
种属种属详情细胞名称酶组合推荐
含推荐浓度
培养基参考文献
Bovine牛脐带 (BUVEC) / Bovine umbilical cord (BUVEC)Collagenase: 0.1%Dulbecco's/Ham F-12Ricken Albert M, Traenkner Anja, Merkwitz Claudia, Hummitzsch Katja, Grosche Jens, Spanel-Borowski Katharina: The short prolactin receptor predominates in endothelial cells of micro- and macrovascular origin, J Vasc Res 44, 19-30, 2007
Bovine肺动脉内皮细胞和平滑肌细胞 / Pulmonary artery endothelial and smooth muscle cellsCollagenase: 0.04-0.05%;
Soybean Trypsin Inhibitor: 0.04%
RPMI-1640Yu, M., McAndrew, R., Al-Saghir, R., Maier, K., Medhora, M., Roman, R. and Jacobs, E.: Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries., J Appl Physiol 93, 1391, 2002
Bovine, (Bos taurus), calf内皮 / EndothelialCollagenase: 1000 u/ml;
Collagenase Type 2: 1000 u/ml
PBS, CMFDel Vecchio, P., Siflinger-Birnboim, A., Belloni, P., Holleran, L., Lum, H., and Malik, A.: Culture and Characterization of Pulmonary Microvascular Endothelial Cells, In Vitro Cell Dev Biol 28A, 711, 1992
Calf内皮 / EndothelialTrypsin: 0.25%HEPESVender, R.: Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension, In Vitro Cell Dev Biol 28A, 403, 1992
Bovine, adult脑动脉,内皮 / Cerebral artery,EndothelialCollagenase: 0.2%HBSSMachi, T., Kassell, N.F., and Scheld, W.M.: Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries, In Vitro Cell Dev Biol 26, 291, 1990
Bovine内皮 / EndothelialTrypsin: 0.05%Krebs Ringer solutionDeNucci, G., Gryglewski, R.J., Warner, T.D., and Vane, J.R.: Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled, Proc Natl Acad Sci U S A 85, 2334, 1988
Bovine内皮,肺动脉 / Endothelial, pulmonary arteryCollagenase: 0.1%CMF-Dulbecco's PBSMartin, T.: Formation of Diacylglycerol by a Phospholipase D-phosphatidate Phosphatase Pathway Specific for Phosphatidylcholine in Endothelial Cells, Biochim Biophys Acta 962, 282, 1988
Bovine内皮 / EndothelialCollagenase Type 2: 0.1%PBSCarson, M.P. and Haudenschild, C.C.: Microvascular Endothelium and Pericytes: High Yield, Low Passage Cultures, In Vitro Cell Dev Biol 22, 344, 1986
Bovine内皮,主动脉 / Endothelial,AorticCollagenase Type 2: 0.1%DMEMKinsella, Michael G. and Wight, Thomas N.: Modulation of Sulfated Proteoglycan Synthesis by Bovine Aortic Endothelial Cells During Migration, J Cell Biol 102, 679, 1986
Bovine内皮 / EndothelialCollagenase: 0.5%DMEM/Ham's F-12Gospodarowicz, D., Massoglia, S., Cheng, J., and Fujii, D.: Effect of Fibroblast Growth Factor and Lipoproteins on the Proliferation of Endothelial Cells Derived From Bovine Adrenal Cortex, Brain Cortex, and Corpus Luteum Capillaries, J Cell Physiol 127, 121, 1986
Bovine内皮,脑动脉 / Endothelial,Brain arteriesCollagenase Type 2: 0.2%Dulbecco's PBSGoetz, I.., Warren, J., Estrada, C., Roberts, E., and Krause, D.: Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain, In Vitro Cell Dev Biol 21, 172, 1985
Calf内皮,平滑肌 / Endothelial,Smooth muscleCollagenase: 0.75%DMEMVoyta, J., Via, D., Butterfield, C., and Zetter, B.: Identification and Isolation of Endothelial Cells Based on Their Increased Uptake of Acetylated-Low Density Lipoprotein, J Cell Biol 99, 2034, 1984
Bovine内皮,角膜 / Endothelial,CornealTrypsin: 0.2%PBS: DMEMScott, D., Murray, J., and Barnes, M.: Investigation of the Attachment of Bovine Corneal Endothelial Cells, Exp Cell Res 144, 472, 1983
Bovine, 2-3 weeks内皮,肺动脉 / Endothelial,Pulmonary arteryCollagenase Type 1: 0.2%RPMI 1640 w/1% Fetal Bovine SerumLee, S., Douglas, W., Deneke, S., and Fanburg, B.: Ultrastructural Changes in Bovine Pulmonary Artery Endothelial Cells Exposed to 80% O2 In Vitro, In Vitro 19, 714, 1983
Bovine内皮,角膜 / Endothelial, CornealTrypsin: 0.05%0.01M Phosphate buffer with 0.02% EDTA;0.9% NaCl (See Reference)Robinson, J. and Gospodarowicz, D.: Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells, J Cell Physiol 117, 368, 1983
Bovine内皮,锁骨下静脉 / Endothelial,Subclavian veinCollagenase Type 1: 0.10%PBSOlander, J., Marasa, J., Kimes, R., Johnston, G., and Feder, J.: An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells, In Vitro 18, 99, 1982
Bovine主动脉,肺动脉 / Aortic,Pulmonary arteryCollagenase Type 2: 0.10%PBSMakarski, J. S.: Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells, In Vitro 17, 450, 1981
Calf, fetal, 4-9 months内皮 / EndothelialCollagenase Type 1: 0.25%PBSRosen, E., Mueller, S., Noveral, J., and Levine, E.: Proliferative Characteristics of Clonal Endothelial Cell Strains, J Cell Physiol 107, 123, 1981
Bovine, adult主动脉 / AortaCollagenase Type 1: 125 u/mlDulbecco's PBS with calcium and magnesiumCotta-Pereira, G., Sage, H., Bornstein, P., Ross, R., and Schwartz, S.: Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis, J Cell Physiol 102, 183, 1980
Bovine, young肺动脉 / Pulmonary arteryCollagenase: 0.1%Medium 199Ryan, U., Mortara, M., and Whitaker, C.: Methods for Microcarrier Culture of Bovine Pulmonary Artery Endothelial Cells Avoiding the Use of Enzymes, Tissue Cell 12, 619, 1980
Bovine包皮 / ForeskinCollagenase: 0.5%Dulbecco's MEM w/10% calf serumFolkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979
Bovine大隐静脉,主动脉 / Saphenous Vein,AortaCollagenase: 0.01%PBSEskin, S., Sybers, H., Trevino, L., Lie, J., and Chimoskey, J.: Comparison of Tissue-Cultured Bovine Endothelial Cells from Aorta and Saphenous Vein, In Vitro 14, 903, 1978
Bovine内皮,胸主动脉,隐静脉 / Endothelial,Thoracic aorta,Saphenous veinsCollagenase Type 2: 0.1%PBSSchwartz, S.M.: Selection and Characterization of Bovine Aortic Endothelial Cells, In Vitro 14, 966, 1978
Bovine肺动脉 / Pulmonary arteryCollagenase Type 2: 0.25%Puck's solutionRyan, U.S., Clements, E., Habliston, D., and Ryan, J.W.: Isolation And Culture of Pulmonary Artery Endothelial Cells, Tissue Cell 10, 535, 1978
Calf内皮 / EndothelialCollagenase Type 2: 0.1%DMEMHoward, B., Macarak, E., Gunson, D., and Kefalides, N.: Characterization of the Collagen Synthesized by Endothelial Cells in Culture, Proc Natl Acad Sci U S A 73 (7), 2361, 1976
犬/狗Dog微血管 / MicrovesselsCollagenase Type 4: 0.1%DMEMGerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels, Brain Res Bull 21, 785, 1988
犬/狗Dog, mongrel, adult内皮,颈静脉 / Endothelium,Jugular veinTrypsin: 0.1%Earle's PBS, CMFFord, J., Burkel, W., and Kahn, R.: Isolation of Adult Canine Venous Endothelium for Tissue Culture, In Vitro 17, 44, 1981
豚鼠Guinea pig, female, 300-350g冠状动脉内皮 / Coronary endothelialCollagenase Type 2: 0.1%see referenceBuxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium, Am J Physiol Heart Circ Physiol 281, H1657-66, 2001
Human血管内皮 / Vascular endothelialCollagenase Type 1: 0.1%HBSSGanguly, A., Zhang, H., Sharma, R., Parsons, S. and Patel, K.: Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions., J Vis Exp 66, e4032, 2012
Human, 18-68 yr角膜内皮 / Corneal endothelialCollagenase: 0.2%;
Neutral Protease: 1.0%
DMEM/F12Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells, Inv Ophthal Visual Sci 48, 614-20, 2007
Human内皮和血管平滑肌 / Endothelial and vascular smooth muscleCollagenase Type 1: 0.2%HBSSMoss, S., Bates, M., Parrino, P. and Woods, TC.: Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue., Ochsner J 7, 133, 2007
Human血管内皮细胞 / HUVECCollagenase Type 1: 0.1%HBSSDavis, J., Crampton, S. and Hughes. C.: Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)., J Vis Exp 3, 183, 2007
Human血管内皮细胞 / HUVECCollagenase Type 4: 0.1%RPMI 1640Silva AP, Kaufmann JE, Vivancos C, Fakan S, Cavadas C, Shaw P, Brunner HR, Vischer U, and Grouzmann E.: Neuropeptide Y expression, localization and cellular transducing effects in HUVEC, Biol Cell 97(6), 457, 2005
Human内皮 / EndothelialCollagenase Type 2: 0.1%DMEMPatel, V., Logan, A., Watkinson, J., Uz-Zaman, S., Sheppard, M., Ramsden, J. and Eggo, M.: Isolation and Characterization of Human Thyroid Endothelial Cells., Am J Physiol Endocrinol Metab 284, E168, 2003
Human食管微血管内皮 / Esophageal microvascular endothelialCollagenase Type 2: 0.2%MCDB-131Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation., Am J Physiol Gastrointest Liver Physiol 285, G1277, 2003
Human结肠内皮细胞 / Colonic endothelial cellsCollagenase Type 2: 0.25%HBSS/5%FBSWang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells, Am J Physiol/Gastro 282, G1088, 2002
Human肺血管内皮细胞 / Pulmonary vascular endothelial cellsNeutral Protease: 1.18 u/ml;
Elastase: 10 u/ml
M199Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM, and Kirkpatrick CJ.: Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro, Exp Mol Pathol 72, 221, 2002


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