Rockland：FemtoMax 超灵敏化学发光HRP底物

发布者：艾美捷科技发布时间：2024-05-17

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FemtoMax 超灵敏化学发光HRP底物是一种极其灵敏、无放射性、增强型基于鲁米诺化学发光底物，用于检测辣根过氧化物酶（HRP）。FemtoMax 设计用于蛋白质印迹(WB)和酶联免疫吸附测定(ELISA)。FemtoMax 可以使用照相胶片或其他成像方法（包括高灵敏度CCD相机）轻松检测飞克级别的抗原。可以将印迹重复暴露于X射线胶片以获得Z佳结果，或者剥离检测试剂并重新检测。在使用FemtoMax 时可使用与Amersham ECL Plus 底物或Pierce SuperSignal West Femto底物相同的印迹条件。

Amersham ECL Plus.png

产品详情：

产品描述:	用于微孔板&膜的化学发光FemtoMax 超敏HRP底物
货号	FEMTOMAX-020
规格	20 mL
应用类型	WB, ELISA
物理状态	液体-透明，无色，无味
储存条件	4℃，防潮和避光

FemtoMax (货号：FEMTOMAX-020)应用实例展示：

Elisa实验：

不同组的血浆MDS-OAβ的浓度检测：将孵育的血浆样品混合物和连续稀释的标准样品加入各自的孔中，并将板在室温下孵育1小时。然后，加入100?L/孔的增强化学发光底物溶液（p/n Femtomax），并使用Victor 3TM多分光光度计检测相对发光单元（RLU）信号。PMID: 33958861

Korean version of Consortium to Establish a Registry for Alzheimer’s disease（CERAD-K）和血浆低聚β淀粉样蛋白（OAβ）的测试：（a） CERAD-K和OAβ浓度的原始分数。（b）与对照组相比，言语流利度、命名、单词记忆/回忆和总分异常的CERAD组（年龄/性别/教育，调整标准差低于?1.5）的OAβ浓度更高。（c）高OAβ组（≥0.78 ng/mL）在命名、单词记忆/回忆/识别和总分方面的异常明显更常见。PMID: 32326061

Western Blot实验：

CK2.3.png

CK2.3对RANKL诱导的破骨细胞生成中p-Erk1/2的影响：CK2.3在RAW264.7细胞中刺激24小时后增加p-Erk 1/2，如（A）蛋白质印迹所测定的。将印迹在3%BSA中孵育1小时以封闭非特异性结合。抗体以1:1000稀释液（在1%BSA中）在4°C下过夜使用。随后与HRP抗兔二抗以1:5000稀释液（在1%BSA中）孵育。印迹在化学发光FemtoMax超敏HRP底物（p/n FemtoMax）中孵育2分钟。PMID:3260129

使用Femtomax的小鼠抗AKT pS473抗体的蛋白质印迹：泳道1：未处理细胞中的非磷酸化AKT。泳道2：PDGF刺激的NIH/3T3细胞裂解物上磷酸化的AKT（用箭头指示在~56kDa处）。上样量：每泳道10?g。一抗：AKT pS473抗体，1:10000，TBS，含0.05% Tween-20和1%BSA，在4°C下1小时。二抗：HRP标记的Gt-a-Mouse IgG（p/n 610-103-121），以1:20000稀释，在4℃下与化学发光FemtoMax 超敏HRP底物（p/n FemtoMax-100）一起使用1小时。批号：25245AB

抗GST标签抗体的蛋白质印迹：泳道1：重组GST标记的重组蛋白5μg；泳道2：重组GST-标记的重组蛋白质2μg；泳道3:重组GST标记的重组蛋白1ug。泳道4:重组GST标记的重组蛋白500 ng。泳道5:重组GST标记的重组蛋白250 ng。泳道6:重组GST标记的重组蛋白100 ng。泳道7:重组GST标记的重组蛋白50 ng。一抗:抗gst抗体，1:1000，在4℃下过夜。二抗：驴二抗，1:10000，室温45分钟。封闭：5%BLOTTO，4°C过夜。预测/观测大小：78 kDa。

FemtoMax HRP.png

使用化学发光FemtoMax HRP底物进行蛋白质印迹检测：rPARP1结构域在4°C下使用一抗兔血清1:500过夜曝光3秒后在11 kDa处检测到。二抗HRP山羊抗兔IgG抗体1:40000。所有孵育均在用于荧光蛋白封闭的封闭缓冲液（p/n MB‐070）中进行。

化学发光FemtoMax 超灵敏HRP底物和竞争对手的比较：GAPDH蛋白以1000、250、62.5、15.6ngs的上样量点在硝化纤维膜上。将膜在室温下封闭一小时。一抗：Rb抗GAPDH1:2000稀释，在4°C下孵育过夜。洗涤后，二抗：Gt抗Rb IgG HRP在室温下以1:50000稀释孵育2小时。检测：化学发光FemtoMax 超敏HRP底物Vs竞争对手1-4。

FemtoMax (货号：FEMTOMAX-020)引文展示：

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