Enzo：ROS-ID Hypoxia/Oxidative Stress Detection Kit

面对复杂多变细胞模型，您是否为如何精确测量细胞内的缺氧及氧化应激水平而困扰？ 

是否为一组检测而需购买2套试剂盒的高昂成本而烦恼？

是否对传统的检测方法繁琐且结果可靠性存疑？   

在生命科学的前沿探索中，准确评估细胞的缺氧与氧化应激状态，对于揭示疾病机理、优化治疗策略至关重要。如今，Enzo Life Sciences倾情推出ROS-ID Hypoxia/Oxidative Stress Detection Kit（ENZ-51042-0125，ENZ-51042-K500），这是一款专为科研人员设计，二合一的创新工具，艾美捷为您推荐的ROS-ID 试剂盒直击这一系列挑战，以其独特的双色荧光标记技术，为您带来前所未有的清晰视野。它不仅能够直观区分缺氧与氧化应激状态，还能确保实验结果的准确性和重复性，让您的研究数据更加信服有力。

* 可单独检测低氧，ROS氧化应激，亦可同时检测！   

高度敏感且特异的荧光探针，可测量活细胞中的缺氧和氧化应激 适用于附着或悬浮细胞系 包括ROS和缺氧诱导剂在内的完整试剂盒 Enzo Life Sciences的ROS-ID?缺氧/氧化应激检测套装旨在功能性检测活细胞（悬浮和附着细胞均适用）中的缺氧和氧化应激水平，可使用荧光显微镜或流式细胞术。该套装包括缺氧（红色）和氧化应激水平（绿色）的荧光探针作为两个主要组成部分。 

实验原理概述：

1、缺氧检测（红色）：缺氧检测试剂（Red Hypoxia Detection Reagent）是一种非荧光或弱荧光的芳香族化合物，含有硝基（NO2）基团。在缺氧细胞中，由于存在硝基还原酶活性，硝基团通过一系列化学反应被转化为羟胺（NHOH）和氨基（NH2）基团，导致原始分子降解并释放出荧光探针，从而在荧光显微镜下呈现红色荧光。

2、氧化应激检测（绿色）：氧化应激检测试剂（Oxidative Stress Detection Reagent）为非荧光、细胞可渗透的总ROS检测染料，能直接与多种活性氧物种反应，如过氧化氢、过氧亚硝酸盐和羟基自由基，生成绿色荧光产物，指示不同类型的ROS/RNS产生。此探针对超氧化物、活性氯或溴物种相对不敏感。  

产品亮点

1、精准检测，双管齐下：该套件同时提供绿色荧光的氧化应激检测试剂与红色荧光的缺氧检测试剂，分别对应细胞内ROS水平与缺氧环境，实现精准定位与定量。

2、广泛适用，兼容性强：无论是贴壁细胞还是悬浮细胞，无论是采用荧光显微镜还是流式细胞仪，ROS-ID 套件都能灵活适应，满足您多样化的实验需求。两种规格（ENZ-51042-K500与ENZ-51042-0125）量身定制，灵活选择，经济高效。

3、操作简便，效率提升：我们对ROS-ID? Hypoxia/Oxidative Stress Detection Mix的准备过程进行了优化，确保实验准备简单快捷，减少实验误差，提升整体研究效率。

4、结果可视化，直观可信：实验设计包含正负对照，确保每次实验都有基准参考，使您的研究结论更具说服力。荧光信号直观显示，细胞状态一目了然，便于数据分析与解读。  

结果分享：

图 1. 氧化应激（A）和低氧（B）检测染料的吸收峰和发射峰分别为504 nm/524 nm和580 nm/595 nm。这些染料可以使用488 nm的氩离子激光器激发，并在大多数实验流式细胞仪的FL3通道（低氧红染料）和FL1通道（氧化应激染料）上检测。 

图 2. HeLa 细胞被种在显微镜载玻片上，第二天按照手册描述，用 DFO（低氧的化学诱导剂）或吡咯烷酮（氧化应激诱导剂）处理 4 小时，温度为 37°C。处理后，载玻片用 PBS 洗涤，盖上盖玻片，并使用 Olympus BX-51 荧光显微镜进行可视化观察。

图3. 培养的人类HeLa和HL-60细胞中缺氧和氧化应激水平的检测。细胞用缺氧诱导剂（DFO）和ROS诱导剂（花青素）处理。每个象限中的数字表示细胞（总体）的百分比。结果表明，缺氧和氧化应激染料具有特异性。  

产品详情：

应用：流式细胞术，荧光显微镜，荧光检测，高通量筛选 

应用说明：此试剂盒设计用于使用贴壁或悬浮细胞进行荧光显微镜和/或流式细胞术。 

质量控制：使用流式细胞术方法进行测试，评估低氧细胞和/或具有高水平总氧化应激的细胞，并结合染料（试剂盒中提供）。还获得显微镜图像。 

数量：对于-K500规格： 500次荧光显微镜分析或100次流式细胞分析。 

对于-0125规格： 125次荧光显微镜分析或25次流式细胞分析。 

使用/稳定性：在适当的储存条件下，试剂盒成分稳定，直到产品标签上注明的日期。 

处理：避免冻融循环。

运输：干冰

短期储存：-20°C 

长期储存：-20°C   

更多产品咨询，欢迎垂询Enzo中国区总代理，艾美捷科技！  400-6800-868，www.amyjet.com  

【附录】发表文章：

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