Worthington 动物脂肪/脂肪组织分离指南

发布者：艾美捷科技发布时间：2022-05-28

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组织分离/原代细胞分离，是酶在组织培养研究和细胞生物学研究中的主要应用。尽管多年来在这些应用中广泛使用酶，但它们在解离和收获中的作用机制尚不清楚。

研究人员在科学文献中搜索有关理想酶和组织解离的最佳条件的信息时，经常会遇到相互矛盾的数据。大部分变化源于细胞外基质的复杂性和动态性，以及在细胞分离应用中使用相对粗糙、不确定的酶制剂。

本指南总结了Worthington如何使用这些酶来完成对应动物脂肪/脂肪组织的解离，以及原代细胞采集的常规操作。本技术指南描述了标准实验室程序；为建立细胞分离方案提供了一种合乎逻辑的实验方法；并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南

动物脂肪/脂肪
种属	种属详情	细胞名称	酶组合推荐，含推荐浓度	培养基	参考文献
禽类		淀粉样蛋白原纤维 Amyloid Fibrils	Collagenase Type 3: 0.02%； Deoxyribonuclease I: 0.001%	50mM Tris	Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges, Methods Mol Biol 1345, 299, 2016
禽类		淀粉样蛋白原纤维 / Amyloid Fibrils	Collagenase Type 3: 0.02%； Deoxyribonuclease I: 0.001%	50mM Tris	Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges, Methods Mol Biol 1345, 299, 2016
熊	Bear, Ursus arctos	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.1%	HBSS	Gehring, J., Rigano, K., Evans, H., Nelson, O., Robbins, C. and Jansen, H.: A Protocol for the Isolation and Cultivation of Brown Bear (Ursus arctos) Adipocytes., Cytotechnology 68, 2177-91, 2016
牛	Bovine	脂肪细胞 / Adipocytes	Collagenase Type 1: 40 u/ml	Krebs-Ringer bicarbonate	Yang, Y., Baldwin, R.: Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue, J Dairy Sci 56, 350, 1973
犬/狗	23-33 kg	基质细胞血管 / Stromal vascular	Collagenase: see reference	Media 199E	Williams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts., Tissue Eng Part A 19, 1295-302, 2013
犬/狗	Canine	肾脂肪来源的细胞 / Renal adipose derived cells	Collagenase Type 1: 0.3%	DMEM	Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
犬/狗	Canine, 20-25 kg	脂肪干细胞 / Adipose stem cells	Collagenase: see reference	Media-199	Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force., J Surg Res 152, 157, 2009
犬/狗	Canine	白色脂肪 / White fat	Collagenase: 0.05%	Kreb's Ringer bicarbonate buffer	DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
马	Equine	脂肪干细胞 / Adipose derived stem cells	Collagenase Type 1: 0.1%	PBS	Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow., Vet Surg 37, 713, 2008
马	Equine, 1-5 year	脂肪干细胞 / Adipose derived stem cells	Collagenase Type 1: 0.1%	PBS	Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells., Vet Surg 36, 613, 2007
鱼	Fish, Atlantic salmon	前脂肪细胞 / Preadipocytes	Collagenase Type 1: 0.1%	HBSS	Todorcevic, M., Vegusdal, A., Gjoen, T., Sundvold, H., Torstensen, B., Kjaer, M. and Ruyter, B.: Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids., Biochim Biophys Acta 1781, 326, 2008
沙鼠	Gerbil of unknown age (also rat, hamster, rabbit, lamb, guinea-pig)	棕色脂肪 / Brown fat	Collagenase Type 1: 0.10%	Bicarbonate buffer	Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
豚鼠	Guinea-pig, adult (also rat, hamster, gerbil, rabbit, lamb)	棕色脂肪 / Brown fat	Collagenase Type 1: 0.10%	Bicarbonate buffer	Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
仓鼠	Hamster, adult(also rat, gerbil, rabbit, lamb, guinea-pig)	棕色脂肪 / Brown fat	Collagenase Type 1: 0.10%	Bicarbonate buffer	Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
仓鼠	Hamster, 5 week-12 month	白色脂肪 / White fat	Collagenase: 0.05%	Kreb's Ringer bicarbonate buffer	DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
人	Human	脂肪来源的干细胞 / Adipose derived stem	Collagenase Type 4: 150-300 u/ml	RPMI-1640	Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat., Curr Protoc Stem Cell Biol 48, e68, 2019
人	Human	脂肪细胞 / Adipocytes	Collagenase Type 2: 0.01-0.5%	DMEM	Tsurumachi, N., Akita, D., Kano, K., Matsumoto, T., Toriumi, T., Kazama, T., Oki, Y., Saito-Tamura, Y., Tonogi, M., Shimizu, N. and Honda, M.: Effect of Collagenase Concentration on The Isolation of Small Adipocytes from Human Buccal Fat Pad., J Oral Sci , , 2018
人	Human	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.1%	HBSS	Wahl, S., Drong, A., Lehne, B., Loh, M., Scott, K., Kunze, S. et al: Epigenome-wide Association Study of Body Mass Index, and the Adverse Outcomes of Adiposity, Nature 541, 81, 2017
人	Human	基质细胞 / Stromal	Collagenase Type 2: 0.075%	DMEM	Duscher, D., Maan, Z., Luan, A., Aitzetmuller, M., Brett, E., Atashroo, D., Whittam, A., Hu, M., Walmsley, G., Houschyar, K., Schilling, A., Machens, H., Gurtner, G., Longaker, M. and Wan, D.: Ultrasound-Assisted Liposuction Provides a Source for Functional Adipose-Derived Stromal Cells., Cytotherapy 19, 1491-1500, 2017
人	Human	间充质基质细胞 / Mesenchymal stromal	Collagenase Type 1: 0.075%	PBS	Lin, Y., Marin-Argany, M., Dick, C., Redhage, K., Blancas-Mejia, L., Bulur, P., Butler, G., Deeds, M., Madden, B., Williams, A., Wall, J., Dietz, A. and Ramirez-Alvarado, M.: Mesenchymal Stromal Cells Protect Human Cardiomyocytes from Amyloid Fibril Damage, Cytotherapy 19, 1426-1437, 2017
人	Human	间充质干细胞 / Mesenchymal stem	Collagenase Type 2: 0.2%	DMEM/F-12	Munir, H., Ward, L., Sheriff, L., Kemble, S., Nayar, S., Barone, F., Nash, G. and McGettrick, H.: Adipogenic Differentiation of Mesenchymal Stem Cells Alters Their Immunomodulatory Properties in a Tissue-Specific Manner., Stem Cells 35, 1636-1646, 2017
人	Human	脂肪来源的间充质干细胞 / Adipose derived mesenchymal stem	Collagenase animal free: 0.1%	PBS	Clark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome., Stem Cell Res Ther 8, 69, 2017
人	Human	脂肪来源的内皮 / Adipose derived endothelial	Collagenase Type 1: 0.1%	DMEM	Szoke, K., Reinisch, A., Ostrup, E., Reinholt, F. and Brinchmann, J.: Autologous Cell Sources in Therapeutic Vasculogenesis: In Vitro and In Vivo Comparison of Endothelial Colony-Forming Cells from Peripheral Blood and Endothelial Cells Isolated from Adipose Tissue., Cytotherapy 18, 242-52, 2016
人	Human	脂肪干细胞 / Adipose stem	Collagenase Type 1: 0.1%	DMEM/F12	Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells, Cytotherapy 18, 371-83, 2016
人	Human	牙髓干细胞 / Dental pulp stem	Collagenase animal free: 0.1%	HBSS	Hu, J., Cao, Y., Xie, Y., Wang, H., Fan, Z., Wang, J., Zhang, C., Wang, J., Wu, C. aand Wang, S.: Periodontal Regeneration in Swine After Cell Injection and Cell Sheet Transplantation of Human Dental Pulp Stem Cells Following Good Manufacturing Practice., Stem Cell Res Ther 7, 130, 2016
人	Human	间充质基质细胞 / Mesenchymal stromal	Collagenase Type 4: 0.2%	PBS	Choudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells., Cytotherapy 17, 359-68, 2015
人	Human	脂肪干细胞 / Adipose derived stem cells	Collagenase Type 2: 0.1%	HBSS	Satish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage., BMC Med Genomics 8, 41, 2015
人	Human, female	脂肪基质细胞 / Adipose stromal	Collagenase Type 1: 0.075%	DMEM	Kinoshita, K., Kuno, S., Ishimine, H., Aoi, N., Mineda, K., Kato, H., Doi, K., Kanayama, K., Feng, J., Mashiko, T., Kurisaki, A. and Yoshimura, K.: Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers., Stem Cells Transl Med 4, 146, 2015
人	Human	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.1%	PBS	Seaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts., Plast Reconstr Surg 136, 189e-199e, 2015
人	Human	脂肪基质细胞-血管 / Adipose stroma-vascular	Neutral Protease: 2.4 u/ml； Collagenase: 250 u/ml	PBS	Esteve, D., Boulet, N., Volat, F., Zakaroff-Girard, A., Ledoux, S., Coupaye, M., Decaunes, P., Belles, C., Gaits-Iacovoni, F., Iacovoni, J., Remaury, A., Castel, B., Ferrara, P., Heymes, C., Lafonta, M. Boloumie, J.: Human White and Brite Adipogenesis is Supported by MSCA1 and is impaired by Immune Cells., Stem Cells 33, 1277-91, 2015
人	Human, adult	脂肪来源的间充质干细胞 / Adipose derived mesenchymal stem	Collagenase Type 2: 0.1%	see reference	Al-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells., Cytotherapy 16, 915, 2014
人	Human, adult	脂肪来源的干细胞 / Adipose derived stem	Collagenase Type 1: 0.15%	DMEM	Koellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities., Cytotherapy 16, 789, 2014
人	Human	间充质基质细胞 / Mesenchymal stromal	Collagenase Type 1: 0.1%	PBS	Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V and De Kock, J: Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods., Cytotherapy 16, 1220-8, 2014
人	Human	脂肪基质细胞干细胞 / Adipose stromal stem	Collagenase animal free: 200 u/ml	DMEM/Hams F-12	Carvalho, P., Gimble, J., Dias, I., Gomes, M. and Reis, R.: Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells., Tiss Eng 19, 473-8, 2013
人	Human	基质细胞血管部分 / Stromal vascular fraction	Collagenase Type 1: 0.075%	PBS	Doi, K., Tanaka, S., Iida, H., Eto, H., Kato, H., Aoi, N., Kuno, S., Hirohi, T. and Yoshimura, K.: Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis., J Tissue Eng Regen Med 7, 864, 2013
人	Human	脂肪来源的干细胞 / Adipose-derived stem	Collagenase Type 1: 0.1%	PBS	Cervelli, V., Scioli, M., Gentile, P., Doldo, E., Bonanno, E., Spagnoli, L. and Orlandi, A.: Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance., Stem Cells Transl Med 1, 206-20, 2012
人	Human	血管周围干细胞 / Perivascular stem	Collagenase Type 2: 0.1%	DMEM	James, A., Zara, J., Corselli, M., Askarinam, A., Zhou, A., Hourfar, A., Nguyen, A., Megerdichian, S., Asatrian, G., Pang, S., Stoker, D., Zhang, X., Wu, B., Ting, K., Peault, B. and Soo, C.: An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering., Stem Cells Transl Med 1, 673, 2012
人	Human	脂肪来源的基质细胞血管 / Adipose derived stromal vascular	Collagenase Type 1: 0.1%	PBS	Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I. and Cervelli, V.: Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery., Stem Cells Transl Med 1, 230-6, 2012
人	Human	基质细胞血管，脂肪细胞 / Stromal vascular, adipocytes	Collagenase Type 1: 0.1%	DMEM	Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I., Curcio, C., Floris, M., Fiaschetti, V., Floris, R. and Cervell, V.: A Comparative Translational Study: The Combined Use of Enhanced Stromal Vascular Fraction and Platelet-Rich Plasma Improves Fat Grafting Maintenance in Breast Reconstruction., Stem Cells Transl Med 1, 341-51, 2012
人	Human, adult	基质细胞血管 / Stromovascular	Collagenase Type 1: 0.1%	PBS with BSA	Hagman, D., Kuzma, J., Larson, I., Foster-Schubert, K., Kuan, L., Cignarella, A., Geamanu, E., Makar, K., Gottlieb, J. and Kratz, M.: Characterizing and Quantifying Leukocyte Populations in Human Adipose Tissue: Impact of Enzymatic Tissue Processing., J Immunol Methods 386, 50, 2012
人	Human	脂肪来源的基质细胞 / Adipose derived stromal	Collagenase Type 1: 0.1%	PBS	Naaijkens, B., Niessen, H., Prins, H., Krijnen, P., Kokhuis, T., de Jong, N,, van Hinsbergh, V., Kamp, O., Helder, M., Musters, R., van Dijk, A. and Juffermans, L.: Human Platelet Lysate as a Fetal Bovine Serum Substitute Improves Human Adipose-Derived Stromal Cell Culture for Future Cardiac Repair Applications., Cell Tissue Res 348, 119, 2012
人	Human	脂肪来源的干细胞 / Adipose derived stem	Collagenase Type 1: 0.1%	DMEM/F-12	Wu, I., Nahas, Z., Kimmerling, K., Rosson, G. and Elisseeff, J.: An Injectable Adipose Matrix for Soft-Tissue Reconstruction., Plast Reconstr Surg 129, 1247, 2012
人	Human, male 40-60 years	脂肪干细胞 / Adipose derived stem cells	Collagenase: 0.25%； Deoxyribonuclease I: 0.002%	PBS	Blasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential, Vasc Cell 3, 5, 2011
人	Human	肾脂肪来源的细胞 / Renal adipose derived cells	Collagenase Type 1: 0.3%	DMEM	Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
人	Human	脂肪来源的间充质 / Adipose derived mesenchymal	Collagenase Type 1: 0.1%	L-DMEM	Yang, X., He, X., He, J., Zhang, L., Su, X., Dong, Z., Xu, Y., Li, Y. and Li, YL.: High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells., J Biomed Sci 18, 59, 2011
人	Human	脂肪来源的干细胞 / Adipose derived stem	Collagenase Type 1: 0.1%	DMEM/Ham's F-12	Yu, G. Floyd, ZE., Wu, X., Halvorsen, Y. and Gimble, J.: Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates., Methods Mol Biol 702, 17-27, 2011
人	Human	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.1%	RPMI	Basu, S., Haghiac, M., Surace, P., Challier, J., Guerre-Millo, M., Singh, K., Waters, T., Minium, J., Presley, L., Catalano, P. and Hauguel-de Mouzon, S.: Pregravid Obesity Associates with Increased Maternal Endotoxemia and Metabolic Inflammation., Obesity 19, 476, 2011
人	Human	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.1%	KRB	Peters, R., Wolf, M., van den Broek, M., Nuvolone, M., Dannenmann, S., Stieger, B., Rapold, R., Konrad, D., Rubin, A., Bertino, J., Aguzzi, A., Heikenwalder, M. and Knuth, A.: Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture., PLoS ONE 5, e15689, 2010
人	Human	脂肪组织来源的干细胞 / Adipose tissue-derived stem	Collagenase Type 2: 1.0%	DMEM/F12	Tan, H., DeFail, A., Rubin, J., Chu, C. and Marra, K.: Novel Multiarm PEG-Based Hydrogels for Tissue Engineering., J Biomed Mater Res 92, 979, 2010
人	Human	基质细胞血管 / Stromal vascular	Collagenase Type 2: 0.1%	HBSS	Zimmerlin, L., Donnenberg, V., Pfeifer, M., Meyer, EM, Peault, B., Rubin, JP and Donnenberg, A.: Stromal Vascular Progenitors in Adult Human Adipose Tissue., Cytometry 77, 22-30, 2010
人	Human	脂肪基质细胞 / Adipose stromal	Collagenase Type 1: 0.1%	DMEM/Hams F-12	Yu, G., Wu, X., Dietrich, M., Polk, P., Scott, LK, Ptitsyn, A. and Gimble, J.: Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes., Cytotherapy 12, 538, 2010
人	Human	脂肪细胞 / Adipocytes	Collagenase: 0.075%	DMEM	Suga, H., Eto, H., Shigeura, T., Inoue, K., Aoi, N., Kato, H., Nishimura, S., Manabe, I., Gonda, K. and Yoshimura, K.: IFATS Collection: Fibroblast Growth Factor-2-Induced Hepatocyte Growth Factor Secretion by Adipose-Derived Stromal Cells Inhibits Postinjury Fibrogenesis Through a c-Jun N-Terminal Kinase-Dependent Mechanism., Stem Cells 27, 238, 2009
人	Human	脂肪基质细胞 / Adipose stromal	Collagenase Type 1: 0.1%	DMEM	Cai, L., Johnstone, B., Cook, T., Tan, J., Fishbein, M., Chen, P. and March, K.: IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function., Stem Cells 27, 230, 2009
人	Human	脂肪组织来源的干细胞 / Adipose tissue-derived stem	Collagenase Type 2: 1.0%	DMEM/F12	Tan, H., Ramirez, C., Miljkovic, N., Li, H., Rubin, J. and Marra, K.: Thermosensitive Injectable Hyaluronic Acid Hydrogel for Adipose Tissue Engineering., Biomaterials 30, 6844, 2009
人	Human	脂肪干细胞 / Adipose derived stem cells	Collagenase Type 1: 0.1%	DMEM	Tandon, N., Goh, B., Marsano, A., Chao, P., Montouri-Sorrentino, C., Gimble, J. and Vunjak-Novakovic, G.: Alignment and Elongation of Human Adipose-Derived Stem Cells in Response to Direct-Current Electrical Stimulation., Conf Proc IEEE Eng Med Biol Soc 2009, 6517-21, 2009
人	Human	脂肪来源的基质细胞 / Adipose derived stromal cells	Collagenase Type 1: see reference	DMEM	Traktuev, D., Merfeld-Clauss, S., Li, J., Kolonin, M., Arap, W., Pasqualini, R., Johnstone, B., and March, K.: A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks, Circ Res 102, 77, 2008
人	Human	基质细胞 / Stromal	Collagenase Type 1: 0.2%	PBS	Minana, M., Carbonell-Uberos, F., Mirabet, V., Marin, S. and Encabo, A.: IFATS Collection: Identification of Hemangioblasts in the Adult Human Adipose Tissue., Stem Cells 26, 2696, 2008
人	Human	基质细胞 / Stromal	Collagenase Type 1: 0.1%； Neutral Protease:	DMEM	Nie, J., Chang, B., Traktuev, D., Sun, J., March, K., Chan, L., Sage, H., Pasqualini, R., Arap, W. and Kolonin, M.: IFATS Collection: Combinatorial Peptides Identify alpha-5 beta-1 Integrin as a Receptor for the Matricellular Protein SPARC on Adipose Stromal Cells., Stem Cells 26, 2735, 2008
人	Human	干细胞 / Stem	Collagenase: 280 u/ml	D-PBS	Pilgaard, L., Lund, P., Rasmussen, J., Fink, T. and Zachar, V.: Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells., Regen Med 3, 705-15, 2008
人	Human	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.2%	HBSS	Bujalska Iwona J, Durrani Omar M, Abbott Joseph, Onyimba Claire U, Khosla Pamela, Moosavi Areeb H, Reuser Tristan T Q, Stewart Paul M, Tomlinson Jeremy W, Walker Elizabeth A, Rauz Saaeha: Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat, J Endocrinol 192, 279-88, 2007
人	Human	脂肪来源的基质细胞 / Adipose derived stromal cells	Collagenase Type 1: 0.15%	DMEM	Schaffler A, Buchler C: Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies, Stem Cells 25, 818-27, 2007
人	Human	脂肪干细胞 / Adipose derived stem cells	Collagenase Type 1: 0.1%	DMEM/F12	Kilroy, G., Foster, S., Wu, X., Ruiz, J., Sherwood, S., Heifetz, A., Ludlow, J., Stricker, D., Potiny, S., Green, P., Halvorsen, Y., Cheatham, B., Storms, R. and Gimble, J.: Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors., J Cell Physiol 212, 702-9, 2007
人	Human	间充质干细胞 / Mesenchymal stem	Collagenase Type 1: 0.1%	HBSS	Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK, J Lipid Res 47, 653-64, 2006
人	Human	前脂肪细胞 / Preadipocytes	Collagenase Type 1: 196 u/ml	M199	Koellensperger, E., Von Heimburg, D., Markowicz, M. and Pallua, N.: Human Serum from Platelet-Poor Plasma for the Culture of Primary Human Preadipocytes., Stem Cells 24, 1218-25, 2006
人	Human	脂肪细胞，基质细胞血管 / Adipocytes, stromal vascular	Collagenase: 0.2%	HBSS	Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture, Mol Biol Cell 16, 1131-41, 2005
人	Human	多能脂肪来源的干细胞 / Multipotent adipose derived stem	Collagenase: 0.2%	DMEM	Rodriguez Anne-Marie, Pisani Didier, Dechesne Claude A, Turc-Carel Claude, Kurzenne Jean-Yves, Wdziekonski Brigitte, Villageois Albert, Bagnis Claude, Breittmayer Jean-Philippe, Groux Herve, Ailhaud Gerard, Dani Christian: Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse, J Exp Med 201, 1397-405, 2005
人	Human	成体干细胞 / Adult stem cells	Collagenase Type 1: 0.1%	PBS	Devireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress., J Biomech Eng 127, 1081, 2005
人	Human, adult, obese	脂肪细胞 / Adipocytes	Collagenase Type 2: 0.1%	DMEM/F12	Seboek, D., Linscheid, P., Zulewski, H., Langer, I., Christ-Crain, M., Keller, U., and Muller, B.: Somatostatin Is Expressed and Secreted by Human Adipose Tissue Upon Infection and Inflammation, J Clin Endocrinol Metab 89(10), 4833, 2004
人	Human, adult, female	前脂肪细胞 / Preadipocytes	Collagenase Type 1: 0.2%	DMEM/F-12	Quickler, M., Sinha, B., Tomlinson, J., Bujalska, I., Stewart, P., Arlt, W.,: Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5, J Endocrinol 183, 331, 2004
人	Human, adult	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.13%	see reference	Fain JN, Madan AK, Hiler ML, Cheema P, and Bahouth SW.: Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans, Endocrinology 145, 2273, 2004
人	Human	基质细胞血管，脂肪细胞 / Stromal vascular, adipocytes	Collagenase: 0.2%	DMEM/F12	Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives, Circulation 109, 656-63, 2004
人	Human	基质细胞血管，脂肪细胞，干细胞 / Stromal vascular, adipocytes, stem	Collagenase: 300 u/ml	PBS	Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells, Circulation 110, 349-55, 2004
人	Human, adult, female, non-obese	脂肪细胞 / Adipocytes	Collagenase Type 2: 0.05%	DMEM	Gesta, S., Lolmede, K., Daviaud, D., Berlan, M., Bouloumie, A., Lafontan, M., Valet, P., and Saulnier-Blache, J.: Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression, Horm Metab Res 35, 158, 2003
人	Human	脂肪细胞 / Adipocytes	Collagenase Type 1: see reference	Saline	Patwardhan, R., Tubbs, R., Leonard, R., Kelly, D., Killingsworth, C., Rollins, D., Smith, W., Ideker, R., and Oakes, W.: Discernment of Adipose versus Nervous Tissue: A Novel Adjunct Solution in Lipomyelomeningocele Surgery, Ped Neurosurg 36, 314, 2002
人	Human, adult, male and female	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.2%	DMEM/F12	McTernan, P., Anderson, L., Anwar, A., Eggo, M., Crocker, J., Barnett, A., Stewart, P., and Kumar, S.: Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences, J Clin Endocrinol Metab 87, 1327, 2002
人	Human	处理过的脂肪抽吸细胞 / Processed lipoaspirate cells	Collagenase Type 1: 0.075%	PBS	Zuk, P., Zhu, M., Ashjian, P., De Ugarte, D., Huang, J., Mizuno, H., Alfonso, Z., Fraser, J., Benhaim, P., and Hedrick, M.: Human adipose tissue is a source of multipotent stem cells, Mol Biol Cell 13, 4279-95, 2002
人	Human	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.2%	HBSS	McTernan PG, Anwar A, Eggo MC, Barnett AH, Stewart PM, Kumar S: Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue, Int J Obes Relat Metab Disord 24, 875-81, 2000
人	Human, adult, male and female	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.1%	DMEM/Ham's F-12	Gottschling-Zelle, H., Birgel, M., Scriba, D., Blum, W., and Hauner, H.: Depot-specific Release of Leptin from Subcutaneous and Omental Adipocytes in Suspension Culture: Effect of Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta1, Eur J Endocrinol 141 (4), 436, 1999
人	Human, adult, male and female	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.1%	DMEM/F12	Zhang, H., Kumar, S., Barnett, A., and Eggo, M.: Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake, J Clin Endocrinol Metab 84, 2550, 1999
人	Human, non-diabetic, male	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.05%	Kreb's Ringer bicarbonate buffer	Anderson, O., Gliemann, J., and Gammeltoft: Receptor Binding and Biological Effect of Insulin in Human Adipocytes, Diabetologia 13, 589, 1977
小鼠	Mouse	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.1%	DMEM	Jones, J., Rabhi, N., Orofino, J., Gamini, R., Perissi, V., Vernochet, C. and Farmer, S.: The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet., Sci Rep 10, 2380, 2020
小鼠	Mouse	脂肪基质细胞 / Adipose stromal	Collagenase Type 1: 0.1%	HBSS	Bowles, A., Strong, A., Wise, R., Thomas, R., Gerstein, B., Dutreil, M., Hunter, R., Gimble, J. and Bunnell, B.: Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis., Stem Cells 35, 532-544, 2017
小鼠	Mouse	脂肪间质基质细胞 / Adipose mesenchyal stromal	Collagenase Type 2: 0.2%	DMEM	Maria, O., Shalaby, M., Syme, A., Eliopoulos, N. and Muanza, T.: Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis., Cytotherapy 18, 1129-45, 2016
小鼠	Mouse	脂肪基质细胞 / Adipose stromal	Collagenase Type 1: 0.1%	PBS	Yao, W., Lay, Y., Kot, A., Liu, R., Zhang, H., Chen, H., Lam, K. and Lane, N.: Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference., Stem Cells 34, 2587-2600, 2016
小鼠	Mouse	脂肪细胞 / Adipocytes	Collagenase: 0.1%	Krebs	Jang, H., Bhasin, S., Guarneri, T., Serra, C., Schneider, M., Lee, M., Guo, W., Fried, S., Pencina, K. and Jasuja, R.: The Effects of A Single Developmentally-Entrained Pulse of Testosterone in Female Neonatal Mice On Reproductive and Metabolic Functions in Adult Life., Endocrinology 156, 3737, 2015
小鼠	Mouse	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.1%	PBS	Seaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts., Plast Reconstr Surg 136, 189e-199e, 2015
小鼠	Mouse, 20 week	基质细胞血管 / Stromal vascular	Collagenase Type 2: 0.2%	RPMI-1640	Kondo, T, Toyoshima, Y., Ishii, Y and Kyuwa, S.: Natural Killer T Cells in Adipose Tissue are Activated in Lean Mice., Exp Anim 62, 319, 2013
小鼠	Mouse	脂肪来源的干细胞 / Adipose derived stem	Collagenase Type 2: 0.1%	DMEM	Takahashi, H., Haraguchi, N., Nishikawa, S., Miyazaki, S., Suzuki, Y., Mizushima, T., Nishimura, J., Takemasa, I., Yamamoto, H., Mimori, K., Ishii, H., Doki, Y. and Mori, M.: Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair., Stem Cells Transl Med 1, 803, 2012
小鼠	Mouse	干细胞和祖 / Stem and progenitor	Collagenase Type 2: 0.2%	HBSS	Han, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells., Blood 115, 957, 2010
小鼠	Mouse, 3 week	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.2%	HBSS	De Matteis, R., Zingaretti, M., Murano, I., Vitali, A., Frontini, A., Giannulis, I., Barbatelli, G., Marcucci, F., Bordicchia, M., Sarzani, R., Raviola, E. and Cinti, S.: In Vivo Physiological Transdifferentiation of Adult Adipose Cells., Stem Cells 27, 2761, 2009
小鼠	Mouse	白色脂肪细胞 / White adipocytes	Collagenase Type 2: 0.1%	DMEM	Wong, K., Szeto, F, Zhang, W., Ye, H., Kong, J., Zhang, Z., Sun, X. and Li, Y: Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins., Am J Physiol/Endo 296, 820, 2009
小鼠	Mouse	脂肪基质细胞 / Adipose stromal	Collagenase Type 2: 0.075%	DMEM	Thangarajah, H., Vial, I., Chang, E., El-Ftesi, S., Januszyk, M., Chang, E., Paterno, J., Neofytou, E., Longaker, M. and Gurtner, G.: IFATS Collection: Adipose Stromal Cells Adopt a Proangiogenic Phenotype Under the Influence of Hypoxia., Stem Cells 27, 266, 2009
小鼠	Mouse	脂肪组织来源的干细胞 / Adipose tissue-derived stem	Collagenase Type 1: 0.075%	Modified Eagles	Cho, K., Park, H., Park, H., Jung, J., Jeon, S., Kim, Y. and Roh, H.: IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model., Stem Cells 27, 259-65, 2009
小鼠	Mouse, C57Bl/6J	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.15%	DMEM/F12	Aoyagi T, Shimba S, Tezuka M: Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes, J Health Sci 51, 21, 2005
小鼠	Mouse, 3-6 day and 2-3 month	脂肪来源的基质细胞 / Adipose-derived stromal cells	Collagenase Type 2: 0.075%	PBS	Cowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T: Adipose-derived adult stromal cells heal critical-size mouse calvarial defects, Nat Biotechnol 22, 560-7, 2004
小鼠	Mouse	基质细胞血管，脂肪细胞 / Stromal vascular, adipocytes	Collagenase: 0.2%	DMEM/F12	Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives, Circulation 109, 656-63, 2004
小鼠	Mouse, C57BL/6J and FVB, male, 8-9 week	脂肪细胞 / Adipocytes	Collagenase: see reference	DMEM	Ruan, H., Zarnowski, MJ., Cushman, S., and Lodish, H.: Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes, J Biol Chem 278, 47585, 2003
小鼠	Mouse, both sexes	脂肪细胞 / Adipocytes	Collagenase: 0.05%	Krebs-Ringer Phosphate HEPES (KRPH)	Nadler, S., Stoehr, J., Rabaglia, M., Schueler, K., Birnbaum, M., and Attie, A.: Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice, Am J Physiol/Endo 281, E1249, 2001
小鼠	Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male	血管内皮 / Vascular endothelial	Collagenase: 0.2%	PBS	Launder, T., Gegen, N., Knedler, A., and Harbeck, R.: The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue, J Immunol Methods 102, 45, 1987
猪	Porcine, female, <1 year	脂肪间充质干细胞 / Adipose mesenchymal stem	Collagenase Type 1: 0.1%	DMEM	Williams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells., Cells Tissues Organs 188, 251, 2008
猪	Porcine, crossbred, male 1-4 day	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.2%	DMEM/F12	Ramsay, T.G.: Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes, J Anim Sci 81, 3008, 2003
猪	Porcine, crossbred	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.067%	Krebs-Ringer	Liang, W. and Mills, S.: Quantitative Analysis of beta-Adrenergic Receptor Subtypes in Pig Tissues., J Anim Sci 80, 963-70, 2002
猪	Porcine, 8-9 week	脂肪 / Adipose	Collagenase Type 1: 300 u/ml	HEPES	Ding, S., McNeel, R., and Mersmann, H.: Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo, Comp Biochem Physiol B 123, 307, 1999
猪	Porcine, neonatal	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.3%	Krebs-Ringer bicarbonate albumin	Wang Y, Fried SK, Petersen RN, Schoknecht PA: Somatotropin regulates adipose tissue metabolism in neonatal swine, J Nutr 129, 139-45, 1999
猪	Porcine, crossbred, 1-3 day	脂肪，基质细胞血管 / Adipose, Stromal-vascular	Collagenase Type 1: 0.2%	DMEM/F12	Suryawan, A., Swanson, L., and Hu, C.: Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture, J Anim Sci 75, 105, 1997
大鼠	Rat, SD, 2-3 month	间充质基质细胞 / Mesenchymal stromal	Collagenase Type 1: 0.1%	DMEM	Dayer, D., Tabar, M., Moghimipour, E., Tabandeh, M., Ghadiri, A., Bakhshi, E., Orazizadeh, M. and Ghafari, M.: Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells, Cytotherapy 19, 937-946, 2017
大鼠	Rat, 9 week	脂肪基质细胞 / Adipose stromal	Collagenase: 0.1%	DMEM	Ohta, Y., Hamaguchi, A., Ootaki, M., Watanabe, M., Takeba, Y., Iiri, T., Matsumoto, N. and Takenaga, M.: Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury., Cytotherapy 19, 839-848, 2017
大鼠	Rat	间充质干细胞 / Mesenchymal stem	Collagenase Type 1: 0.075%	DMEM	Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G and Karaoz, E: Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model., Cytotherapy 17, 543-59, 2015
大鼠	Rat, SD, 5 month	间充质基质细胞 / Mesenchymal stromal	Collagenase Type 2: 0.075%	DMEM	Veronesi, F, Torricelli, P, Della, B, Pagani, S and Fini, M: In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells., Cytotherapy 17, 215-23, 2015
大鼠	Rat	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.3%	Krebs-Ringer	Ogasawara, J., Izawa, T., Sakurai, T., Shirato, K., Ishibashi, Y., Ohira, Y., Ishida, H., Ohno, H. and Kizaki, T.: Habitual Exercise Training Acts as a Physiological Stimulator for Constant Activation of Lipolytic Enzymes in Rat Primary White Adipocytes., Biochem Biophys Res Commun 464, 348-53, 2015
大鼠	Rat, adult	基质细胞血管 / Stromal vascular	Collagenase Type 1: 0.2%	PBS	Leblanc, A., Nguyen, Q., Touroo, J., Aird, A., Chang, R., Ng, C., Hoying, J. and Williams, S.: Adipose-derived Cell Construct Stabilizes Heart Function and Increases Microvascular Perfusion in an Established Infarct., Stem Cells Transl Med 2, 896-905, 2013
大鼠	Rat, male, 300g	脂肪细胞 / Adipocytes	Collagenase Type 2: 0.33%	RPMI 1640	Thompson, A., Nunez, M., Davidson, R., Horm, T., Schnittker, K., Hart, M., Suarez, A. and Tsao, T.: Mitigation of Isolation-Associated Adipocyte Interleukin-6 Secretion Following Rapid Dissociation of Adipose Tissue., J Lipid Res 53, 2797, 2012
大鼠	Rat, Lewis, male	肾脂肪来源的细胞 / Renal adipose derived cells	Collagenase Type 1: 0.3%	DMEM	Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
大鼠	Rat, SD, neonatal	棕色脂肪细胞 / Brown adipocytes	Collagenase Type 4: 0.1%； Neutral Protease: 0.1%； Trypsin: 0.05%	PBS	Liu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.: Efficient Isolation of Cardiac Stem Cells from Brown Adipose., J Biomed Biotechnol 2010, 104296, 2010
大鼠	Rat	脂肪来源的干细胞 / Adipose derived stem	Collagenase Type 1: 0.1%	DMEM	Wei, X., Du, Z., Zhao, L., Feng, D., Wei, G., He, Y., Tan, J., Lee, W., Hampel, H., Dodel, R., Johnstone, B., March, K., Farlow, M. and Du, Y.: IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats., Stem Cells 27, 478, 2009
大鼠	Rat	脂肪组织来源的干细胞 / Adipose tissue-derived stem	Collagenase Type 2: 0.075%	DMEM	Tomiyama, K., Murase, N., Stolz, D., Toyokawa, H., O'Donnell, D., Smith, D., Dudas, J., Rubin, J. and Marra, K.: Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells Into Adipose Tissue., Stem Cells 26, 330, 2008
大鼠	Rat, Wistar, 4 week	脂肪细胞 / Adipocytes	Collagenase: 0.2%	Ham's F12	Aoki, S., Toda, S., Sakemi, T., and Sugihara, H.: Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro, Cell Struct Funct 28, 55, 2003
大鼠	Rat, SD	脂肪细胞 / Adipocytes	Collagenase Type 1: 0.2%	KRHB	Mora, S., Yang, C., Ryder, J., Boeglin, D and Pessin, J: The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency, Endocrinology 142, 1999, 2001
大鼠	Rat, SD, male, 4-7 weeks	棕色脂肪细胞 / Brown adipocytes	Deoxyribonuclease I: 0.5%	DMEM	Omatsu-Kanbe, M., and Matsuura, H.: Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes, J Physiol 521 (3), 601, 1999
大鼠	Rat	脂肪细胞 / Adipocytes	Collagenase Type 2: 0.2%	DMEM /F-12	Serrero, G: Primary Culture in Defined Medium of Adipocyte Precursors,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 11B:6.1, 1995
大鼠	Rat, SD, male, 130-160 g	脂肪，附睾脂肪垫 / Adipose，Epididymal fat pads	Collagenase: 0.3%	Kreb's-Ringer bicarbonate buffer modified	Charron, M.J. and Kahn, B.B.: Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo, J Biol Chem 265, 7994, 1990
大鼠	Rat, 3 day	前脂肪细胞 / Preadipocytes	Collagenase Type 3: 0.10%	Parker Medium 199	Gaben-Cogneville, A., Poussin, B., Chamblier, M., Forgue-Fafitte, M., and Rosselin, G.: Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture, Biochim Biophys Acta 968, 231, 1988
大鼠	Rat, SD, various weights and ages	棕色脂肪细胞，肩胛间和宫颈贮库 / Brown adipocytes，Interscapular & cervical depots	Collagenase: 0.2%； Soybean Trypsin Inhibitor: 0.3%	Krebs Ringer bicarbonate buffer	Woodward, Julie A. and Saggerson, E.: Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline, Biochem J 238, 395, 1986
大鼠	Rat, CD, male, 150-200 g	脂肪细胞，附睾脂肪垫 / Adipocytes，Epididymal fat pads	Collagenase: 0.1%	Krebs Ringer bicarbonate buffer	Pessin, J.E., Gitomer, W., Oka, Y., Oppenheimer, C.L., and Czech, M.P.: ?-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes, J Biol Chem 258, 7386, 1983
大鼠	Rat, Wistar, albino, male, 100-140 g	脂肪细胞，附睾脂肪垫 / Adipocytes，Epididymal-fat pads	Collagenase: 0.3%	Kreb's Ringer	Green, A. and Newsholme, E.: Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites, Biochem J 180, 365, 1979
大鼠	Rat, Fischer, 344, male, 9 - 13 week	白色脂肪 / White fat	Collagenase: 0.3%	Kreb's Ringer bicarbonate buffer	Stiles, J.W., Francendese, A.A. and Masoro, E.J.: Influence of Age on Size and Number of Fat Cells in the Epididymal Depot, Am J Physiol 229, 1561, 1975
大鼠	Rat, Wistar, male, 5 week-16 month	白色脂肪 / White fat	Collagenase: 0.05%	Kreb's Ringer bicarbonate buffer	DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
大鼠	Rat (CFE), albino, female	棕色脂肪 / Brown fat	Collagenase Type 1: 0.10%	Bicarbonate buffer	Fain, J., Reed, N., and Saperstein, R.: Isolation and Metabolism of Brown Fat Cells, J Biol Chem 242, 1887, 1967
大鼠	Rat, SD, male, 160-210 g	胖的 / Fat	Collagenase: 0.3%	Albumin-bicarbonate buffer	Rodbell, M.: Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis, J Biol Chem 239 (2), 375, 1964
松鼠	Squirrel	棕色脂肪细胞 / Brown adipocytes	Collagenase Type 2: 0.16%	Krebs-Ringer phosphate	McFarlane, S., Mathers, K. and Staples, J.: Reversible Temperature-Dependent Differences in Brown Adipose Tissue Respiration During Torpor in a Mammalian Hibernator., Am J Physiol Regul Integr Comp Physiol 312, R434-R442, 2017