Cell Biolabs：His标签蛋白ELSIA检测试剂盒

His标签，也称为多组氨酸标签（polyhistidine），His6标签或hexa组氨酸标签，这是一种在转染的细胞中目标蛋白的C端或N端上由至少6个组氨酸残基链接上所组成的氨基酸序列。

His-Tag是目前用于纯化的融合标签中应用最广泛的特点：

a：标签较短可置于蛋白的N端或者C端

b：对蛋白的功能及下游应用影响小

c：免疫原性低，可以和多种标签连用

d：纯化方式非常简单方便

这些属性使His标签成为重组蛋白生产和分离的流行标签。然而，对于未纯化的样本，His标签蛋白的准确定量样品仍然很困难。

作为专业的生命科学医药原料供应商，Cell Biolabs中国区金牌代理，艾美捷科技为您推荐：His-Tag 蛋白ELISA 试剂盒，这是一种基于竞争性ELISA测定法，专为快速检测和定量His标签蛋白样品（C 端或 N 端）而开发。

引用文献：

1.Helalat, S.H. et al. (2020). Investigating the efficacy of UVSE protein at repairing CPD and 6–4 pp DNA damages in human cells. J Photochem Photobiol B. doi: 10.1016/j.jphotobiol.2020.111843.

2.Huang, Y. et al. (2020). Dual-mechanism based CTLs infiltration enhancement initiated by Nanosapper potentiates immunotherapy against immune-excluded tumors. Nat Commun. 11(1):622. doi: 10.1038/s41467-020-14425-7.

3.Karamitros, C.S. et al. (2020). Bacterial expression systems for enzymatic activity in droplet-based microfluidics. Anal Chem. doi: 10.1021/acs.analchem.9b04969.

4.Arnett, C. et al. (2019). Expression and secretion of active Moringa oleifera coagulant protein in Bacillus subtilis. Appl Microbiol Biotechnol. doi: 10.1007/s00253-019-10141-5.

5.Liu, Q. et al. (2018). Nanoparticle-Mediated Trapping of Wnt Family Member 5A in Tumor Microenvironments Enhances Immunotherapy for B-Raf Proto-Oncogene Mutant Melanoma. ACS Nano. 12(2):1250-1261. doi: 10.1021/acsnano.7b07384.

6.Ibarra-Sánchez, L.A. et al. (2018). Antimicrobial behavior of phage endolysin PlyP100 and its synergy with nisin to control Listeria monocytogenes in Queso Fresco. Food Microbiol. 72:128- 134. doi: 10.1016/j.fm.2017.11.013.

7.Reyes-Corona, D. et al. (2017). Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion. PLoS One. 12(11):e0188239. doi: 10.1371/journal.pone.0188239.

8.Warnock, N.D. et al. (2017). Nematode neuropeptides as transgenic nematicides. PLoS Pathog. 13(2):e1006237. doi: 10.1371/journal.ppat.1006237.

9.Rinaldo, A. R. et al. (2015). A grapevine anthocyanin acyltransferase, transcriptionally regulated by VvMYBA, can produce most acylated anthocyanins present in grape skins. Plant Physiol. 169:1897-916.

10.Akiyama, Y. et al. (2014). The identification of affinity peptide ligands specific to the variable region of human antibodies. Biomed Res. 35:105-116.

11.Dong, Y. et al. (2013). HMGB1 protein does not mediate the inflammatory response in spontaneous spinal cord regeneration: a hint for CNS regeneration. J. Biol. Chem. 288:18204- 182128.

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