Southern Biotech：SBA 小鼠单克隆抗体分型试剂盒（HRP标记）

单抗分类鉴定首选

在单克隆抗体的制备过程中，杂交瘤细胞的筛选和鉴别是最重要的步骤。首先需要筛选出正确融合的杂交瘤细胞，再筛选出能分泌目标抗体的杂交瘤细胞，最终将筛选出来的杂交瘤细胞进行扩大培养，制备大量单克隆抗体。在进行杂交瘤细胞的筛选时，需要鉴定这类杂交瘤细胞培养上清液中单克隆抗体的类别和亚型，最常用的是ELISA法，因其快速、简便、灵敏度高、特异性强、易于标准化等优点，已得到广泛应用。

单克隆抗体分型ELISA试剂盒应用

单克隆抗体分型ELISA试剂盒运用双抗体夹心法原理，先用Ig捕获二抗在96孔板上包被固相，然后加入待测的杂交瘤细胞培养上清液，其中的目标蛋白便会与96孔板上的固相二抗结合，洗板去除游离物质后加入用于筛选杂交瘤细胞的标记二抗孵育，洗板去除游离物质，加入显色底物显色，在450nm处测定OD值，进行定性及定量分析。

SBA单克隆抗体分型试剂盒

SouthernBiotech单克隆抗体分型试剂盒专为小鼠单克隆抗体的分型设计。分型试剂盒包含纯化的抗小鼠Ig捕获抗体和HRP或AP偶联的抗小鼠Ig、IgA、IgG1、IgG2a或IgG2c、IgG2b、IgG3、IgM、kappa和lambda抗体，用于检测。这些也可用于血清、上清和腹水等样品中小鼠免疫球蛋白水平的定量研究。

试剂盒有多种形式，可用于检测表达Igh1-a等位基因(如BALB/c)和Igh1-b等位基因(如C57BL/6、C57BL/10、SJL、NOD)的小鼠的免疫球蛋白。使用我们的小鼠免疫球蛋白面板与我们的单克隆抗体分型试剂盒可以生成可靠的标准曲线。

SBA克隆分型系统- HRP试剂盒（货号5300-05）含有2.5 mg捕获抗体和1.0 mL HRP偶联抗小鼠Ig、小鼠IgA、小鼠IgG1、小鼠IgG2a、小鼠IgG2b、小鼠IgG3、小鼠IgM、小鼠κ、小鼠λ和ABTS底物。

SBA单克隆抗体分型试剂盒优势

操作简便，结果准确，易于分析；

可用于初筛抗体高分泌杂交瘤细胞株，鉴定阳性克隆细胞的特异性抗体分型(IgA、IgM、IgG1、IgG2a、IgG2b、IgG3), 鉴定抗体kappa、lambda轻链异构体以及普通抗体分型ELISA研究；

性价比高，搭配使用可进行1000次以上样本检测；

每批试剂盒均经过ELISA检测以确保批间一致性。

其中5300-01套装内含有小鼠源IgA、IgM、IgG1、IgG2a、IgG2b、IgG3免疫球蛋白标准品，每种均为1ml（浓度为100μg/ml）；而5300-01B套装内用IgG2b替换了5300-01里的IgG2c。而每种SBA单克隆抗体分型试剂盒则包含了1ml亲和纯化山羊抗小鼠Ig捕获二抗（用于包被96孔板），1ml酶标记山羊抗小鼠Ig二抗（用于初筛杂交瘤细胞），1ml×8酶标记山羊抗小鼠IgA, IgM, IgG1, IgG2a, IgG2b, IgG3, Ig kappa，Ig lambda二抗，酶反应底物及操作手册；此外还有配套Ig标准品可供选择。

相关产品推荐

5300-05 部分引文如下，其他产品的引文请参考官网或联系艾美捷：

1. Tseng T, Chung C. A monoclonal antibody recognizing the specific epitope of aflatoxin analogs. Bot Bull Acad Sin. 1990;31:279-84. (ELISA - Isotyping)

2. Yu L, Lee KK, Ens K, Doig PC, Carpenter MR, Staddon W, et al. Partial characterization of a Candida albicans fimbrial adhesin. Infect Immun. 1994;62:2834-42. (ELISA - Isoyping)

3. Esteban MR, Giovinazzo G, de la Hera A, Goday C. PUMA1: a novel protein that associates with the centrosomes, spindle and centromeres in the nematode Parascaris. J Cell Sci. 1998;111:723-35. (ELISA - Isotyping)

4. Gil OD, Zhang L, Chen S, Ren YQ, Pimenta A, Zanazzi G, et al. Complementary expression and heterophilic interactions between IgLON family members neurotrimin and LAMP. J Neurobiol. 2002;51:190-204. (ELISA - Isotyping)

5. Sánchez MD, Pierson TC, McAllister D, Hanna SL, Puffer BA, Valentine LE, et al. Characterization of neutralizing antibodies to West Nile virus. Virology. 2005;336:70-82. (ELISA - Isotyping)

6. Mazzarotto GA, Raboni SM, Stella V, Carstensen S, de Noronha L, Levis S, et al. Production and characterization of monoclonal antibodies against the recombinant nucleoprotein of Araucaria hantavirus. J Virol Methods. 2009;162:96-100. (ELISA - Isotyping)

7. Wang C, deVries S, Camboni M, Glass M, Martin PT. Immunization with the SDPM1 peptide lowers amyloid plaque burden and improves cognitive function in the APPswePSEN1(A246E) transgenic mouse model of Alzheimer's disease. Neurobiol Dis. 2010;39:409-22. (ELISA - Isotyping & Serum)

8. Vassiliadis E, Veidal SS, Simonsen H, Larsen DV, Vainer B, Chen X, et al. Immunological detection of the type V collagen propeptide fragment, PVCP-1230, in connective tissue remodeling associated with liver fibrosis. Biomarkers. 2011;16:426-33. (ELISA - Isotyping)

9. Liu Z, Song C, Li Y, Liu F, Zhang K, Sun Y, et al. Development of highly sensitive chemiluminescence enzyme immunoassay based on the anti-recombinant HC subunit of botulinum neurotoxin type A monoclonal antibodies. Anal Chim Acta. 2012;735:23-30. (ELISA - Isotyping)

10. Han Z, Zhao F, Shao Y, Liu X, Kong X, Song Y, et al. Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein. Virus Res. 2013;171:54-64. (ELISA - Isotyping)

11. Wongwairot S, Kueakhai P, Changklungmoa N, Jaikua W, Sansri V, Meemon K, et al. Monoclonal antibody against recombinant Fasciola gigantica cathepsin L1H could detect juvenile and adult cathepsin Ls of Fasciola gigantica. Parasitol Res. 2015;114:133-40. (ELISA - Isotyping)

12. Ren J, Zhu Y, Zhou Y, Lv C, Yan H, Ma L, et al. Identification of three antigen epitopes on the nucleocapsid protein of the genotype C of bovine parainfluenza virus type 3. Vet Microbiol. 2015;178:61-9. (ELISA - Isotyping)

13. Guo C, Tang Y, Qi Z, Liu Y, Zhao X, Huo X, et al. Development and characterization of a panel of cross-reactive monoclonal antibodies generated using H1N1 influenza virus. Immunobiology. 2015;220:941-6. (ELISA - Isotyping)

14. Li C, Liang W, Liu W, Yang D, Wang H, Ma W, et al. Identification of a conserved linear epitope using a monoclonal antibody against non-structural protein 3B of foot-and-mouth disease virus. Arch Virol. 2016;161:365-75. (ELISA - Isotyping)

15. Gu J, Wang L, Jin Y, Lin C, Wang H, Zhou N, et al. Characterization of specific antigenic epitopes and the nuclear export signal of the Porcine circovirus 2 ORF3 protein. Vet Microbiol. 2016;184:40-50. (ELISA - Isotyping)

16. Hansen NU, Willumsen N, Sand JM, Larsen L, Karsdal MA, Lemming DJ. Type VIII collagen is elevated in diseases associated with angiogenesis and vascular remodeling. Clin Biochem. 2016 May 24. doi: 10.1016/j.clinbiochem.2016.05.023. [Epub ahead of print]. (ELISA - Isotyping)

17. Cascalho M, Wong J, Wabl M. VH gene replacement in hyperselected B cells of the quasimonoclonal mouse. J Immunol. 1997;159:5795-801. (ELISA - Serum)

18. Kikuchi T, Hackett NR, Crystal RG. Cross-strain protection against clinical and laboratory strains of Pseudomonas aeruginosa mediated by dendritic cells genetically modified to express CD40 ligand and pulsed with specific strains of Pseudomonas aeruginosa. Hum Gene Ther. 2001;12:1251-63. (ELISA - Serum)

19. Wu X, Jiang N, Deppong C, Singh J, Dolecki G, Mao D, et al. A role for the Cr2 gene in modifying autoantibody production in systemic lupus erythematosus. J Immunol. 2002;169:1587-92. (ELISA - Serum)

20. Ruzek MC, Hawes M, Pratt B, McPherson J, Ledbetter S, Richards SM, et al. Minimal effects on immune parameters following chronic anti-TGF-β monoclonal antibody administration to normal mice. Immunopharmacol Immunotoxicol. 2003;25:235-57. (ELISA - Serum)

21. Olson TS, Bamias G, Naganuma M, Rivera-Nieves J, Burcin TL, Ross W, et al. Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease. J Clin Invest. 2004;114:389-98. (ELISA - Serum & Supernatant)

22. Coughlin JJ, Stang SL, Dower NA, Stone JC. RasGRP1 and RasGRP3 regulate B cell proliferation by facilitating B cell receptor-Ras signaling. J Immunol. 2005;175:7179-84. (ELISA - Serum)

23. Perchonock CE, Fernando MC, Quinn WJ 3rd, Nguyen CT, Sun J, Shapiro MJ, et al. Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006;26:6005-15. (ELISA - Serum)

24. Travis MA, Reizis B, Melton AC, Masteller E, Tang Q, Proctor JM, et al. Loss of integrin αvβ8 on dendritic cells causes autoimmunity and colitis in mice. Nature. 2007;449:361-5. (ELISA - Serum)

25. Bell JJ, Divekar RD, Ellis JS, Cascio JA, Haymakker CL, Jain R, et al. In trans T cell tolerance diminishes autoantibody responses and exacerbates experimental allergic encephalomyelitis. J Immunol. 2008;180:1508-16. (ELISA - Serum)

26. Bolduc A, Long E, Stapler D, Cascalho M, Tsubata T, Koni PA, et al. Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas. J Immunol. 2010;185:220-30. (ELISA - Serum)

27. Bohgaki T, Bohgaki M, Cardoso R, Panier S, Zeegers D, Li L, et al. Genomic instability, defective spermatogenesis, immunodeficiency, and cancer in a mouse model of the RIDDLE syndrome. PLoS Genet. 2011;7(4):e1001381. (ELISA - Serum & Supernatant)

28. Huang C, Hatzi K, Melnick A. Lineage-specific functions of Bcl-6 in immunity and inflammation are mediated by distinct biochemical mechanisms. Nat Immunol. 2013;14:380-8. (ELISA - Serum)

29. Huang Q, Perlman H, Birkett R, Doyle R, Fang D, Haines GK, et al. CD11c-mediated deletion of Flip promotes autoreactivity and inflammatory arthritis. Nat Commun. 2015;6:7086. (ELISA - Serum)

30. Ying Z, Mei M, Zhang P, Liu C, He H, Gao F, et al. Histone arginine methylation by PRMT7 controls germinal center formation via regulating Bcl6 transcription. J Immunol. 2015;195:1538-47. (ELISA - Serum)

31. Benhamron S, Pattanayak SP, Berger M, Tirosh B. mTOR activation promotes plasma cell differentiation and bypasses XBP-1 for immunoglobulin secretion. Mol Cell Biol. 2015;35:153-66. (ELISA - Serum & Supernatant)

32. Zanotti KJ, Maul RW, Castiblanco DP, Yang W, Choi YJ, Fox JT, et al. ATAD5 deficiency decreases B cell division and Igh recombination. J Immunol. 2015;194:35-42. (ELISA - Serum)

33. Chen J, Li Z, Petersen E, Liu W, Zhu X. Co-administration of interleukins 7 and 15 with DNA vaccine improves protective immunity against Toxoplasma gondii. Exp Parasitol. 2016;162:18-23. (ELISA - Serum)

34. Zhao Y, Li Z, Chen J, Sun X, Liu S, Zhu X, ey al. Protective efficacy of pVAX-RON5p against acute and chronic infections of Toxoplasma gondii in BALB/c mice. Exp Parasitol. 2016;163:24-30. (ELISA - Serum)

35. Corcos D, Iglesias A, Dunda O, Bucchini D, Jami J. Allelic exclusion in transgenic mice expressing a heavy chain disease-like human μ protein. Eur J Immunol. 1991;21:2711-6. (ELISA - Supernatant)

36. Ishida K, Yoshimura K. Characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of Angiostrongylus cantonensis. Parasite Immunol. 1992;14:633-44. (ELISA - Supernatant)

37. Yamada T, Mitani T, Yorita K, Uchida D, Matsushima A, Iwamasa K, et al. Abnormal immune function of hemopoietic cells from alymphoplasia (aly) mice, a natural strain with mutant NF-κB-inducing kinase. J Immunol. 2000;165:804-12. (ELISA - Supernatant)

38. Wittman VP, Woodburn D, Nguyen T, Neethling FA, Wright S, Weidanz JA. Antibody targeting to a class I MHC-peptide epitope promotes tumor cell death. J Immunol. 2006;177:4187-95. (ELISA - Supernatant)

39. Verma B, Hawkins OE, Neethling FA, Caseltine SL, Largo SR, Hildebrand WH, et al. Direct discovery and validation of a peptide/MHC epitope expressed in primary human breast cancer cells using a TCRm monoclonal antibody with profound antitumor properties. Cancer Immunol Immunother. 2010;59:563-73. (ELISA - Supernatant)

40. Zhang L, Zhang H, Fan Z, Zhou X, Yu L, Sun H, et al. Identification of a conserved linear B-cell epitope of Streptococcus dysgalactiae GapC protein by screening phage-displayed random peptide library. PLoS One. 2015;10(6):e0131221. (ELISA - Supernatant & Ascites)

41. Zhang J, Li H, Huo R, Zhai T, Li H, Sun Y, et al. Paeoniflorin selectively inhibits LPS-provoked B-cell function. J Pharmacol Sci. 2015;128:8-16. (ELISA - Supernatant)

42. Zhang L, Zhou X, Fan Z, Tang W, Chen L, Dai J, et al. Identification of a conserved B-cell epitope on the GapC protein of Streptococcus dysgalactiae. Microb Pathog. 2015;83-84:23-8. (ELISA - Supernatant & Ascites)