PBL Assay Science：人IFN-α ELISA试剂盒

IFN-α（Interferon-alpha，IFN-Alpha）是指干扰素α，它是一种类型的细胞因子，属于干扰素家族。干扰素是哺乳动物细胞因子家族，具有调节免疫反应和抗病毒能力的作用。它们由大多数细胞类型合成和分泌，以响应病原体。除了抗病毒特性外，干扰素还被证明具有抗增殖、免疫调节和许多其他活性。

当干扰素与其同源受体相互作用时，信号在细胞内迅速传递，通常产生抗病毒状态。I型IFN促进的主要信号转导级联反应是JAK1-STAT通路。该信号转导途径的激活导致基因表达增加，包括保护细胞免受病毒感染的（2'-5'）寡腺苷酸合成酶、Mx蛋白和蛋白激酶R（PKR）。   

我们推荐PBL Assay Science的人IFN-α ELISA试剂已被开发用于使用夹心酶联免疫测定法（ELISA）量化细胞或组织培养基（TCM）中的人IFN-α的水平。作为PBL Assay Science在中国区域的代理商，艾美捷科技将为中国客户提供全面的PBL Assay Science产品以及客户订制化服务。欢迎大家随时联系我们。

引用文献（Citations）：

1. Jiang, S., et al., (2023), "Double-modified oncolytic adenovirus armed with a recombinant interferon-like gene enhanced abscopal effects against malignant glioma", Neuro-Oncology Advances, vdad117, DOI: 10.1093/noajnl/vdad117

2. Zhang, Z., et al.,(2021), "IRF7 and RNH1 are modifying factors of HIV-1 reservoirs: a genome-wide association analysis", BMC Medicine, 19:282, DOI:10.1186/s12916-021-02156-5.

3. Sabree, SA., et al., (2021), "Direct and indirect immune effecrs of CMP-001, a virus-like particle containing a TLR-9 agnoist", Journal of ImmunoTherapy of Cancer, 9:e002484, DOI: 10.1136/jitc-2021-002484

4. Evans, Jay, et al. (2019). Synthetic Toll-like Receptors 7 and 8 Agonists: Structure–Activity Relationship in the Oxoadenine Series. ACS Omega, 13 pgs. PMID: 31572869.

5. Kondo, Yasuteru, et al. (2019). The reduction of miR146b-5p in monocytes and T cells could contribute to the immunopathogenesis of hepatitis C virus infection. Scientific Reports, 13 pgs. PMID: 31527804.

6. Loughran, Sinead, et al. (2018). Influenza infection directly alters innate IL-23 and IL-12p70 and subsequent IL-17A and IFN-γ responses to pneumococcus in vitro in human monocytes. PLOS One, 17 pgs. PMID: 30192848.

7. Moskwa, Sylwia, et al. (2018). Innate Immune Response to Viral Infections in Primary Bronchial Epithelial Cells is Modified by the Atopic Status of Asthmatic Patients. AAIR,11 pgs. PMID: 29411555.

8. Svensson, Alexandra, et al. (2017). Maturation-Dependent Expression of AIM2 in Human B-Cells. PLOS One,13 pgs. PMID: 28809949.

9. Wang, et al. (2017). Intracellular DNA sensing pathway of cGAS-cGAMP is decreased in human newborns and young children. Molecular Immunology. PMID: 28412547.

10. Schuh, Elisabeth, et al. (2017). Human Plasmacytoid Dendritic Cells Display and Shed B Cell Maturation Antigen upon TLR Engagement.Journal of Immunology, 9 pgs. PMID: 28283566.

11. Herawati, Neng, et al. (2017). Overproduction, Characterization, and Preliminary Antiproliferative Activity Determination of Non-Tagged Recombinant Human Interferon Alpha-2a Produced in Pichia pastoris. Nusantara Bioscience, 5 pgs. PMID: no PMID.

12. Abd-Elraheem, Sabah, et al. (2017). Estimation of Serum Interferon Alpha and Toll-Like Receptors 3 (TLRs 3) Gene Expression in Egyptian Patients with Liver Cirrhosis. JBC, 8 pgs. PMID: no PMID.

13. Hammerstad, Sara, et al. (2016). Hepatitis C Virus E2 Protein Induces Upregulation of Il-8 Pathways and Production of Heat Shock Proteins in Human Thyroid Cells. Endocrine Society,18 pgs. PMID: 27860532.

14. Lee, Joo Youn, et al. (2016). Circulating Exosomes from Patients with Systemic Lupus Erythematosus Induce a Proinflammatory Immune Response.Arthritis Research & Therapy, 8 pgs. PMID: 27852323.

15. Bam, Rujuta, et al. (2016). TLR7 Agonist GS-9620 Is a Potent Inhibitor of Acute HIV-1 Infection in Human Peripheral Blood Mononuclear Cells. Antimicrobial Agents and Chemotherapy,14 pgs. PMID: 27799218.

16. Millrine, David, et al. (2016). Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production. PNAS, 6 pgs. PMID: 27601648.

17. Jang, Mihue, et al. (2016). A RNA Nanotechnology Platform for a Simultaneous Two-in-One siRNA Delivery and its Application in Synergistic RNAi Therapy. Scientific Reports,16 pgs. PMID: 27562435.

18. Nantachit, Nattika, et al. (2016). Responses of Primary Human Nasal Epithelial Cells to EDIII-DENV Stimulation: The First Step to Intranasal Dengue Vaccination. Virology Journal, 8 pgs. PMID: 27538960.

19. Perez-Pinera, Pablo, et al. (2016). Synthetic Biology and Microbioreactor Platforms for Programmable Production of Biologics at the Point-of-Care. Nature Communications,10 pgs. PMID: 27470089.

20. Goodier, Martin, et al. (2016). Influenza Vaccination Generates Cytokine-Induced Memory-Like NK Cells: Impact of Human Cytomegalvirus Infection. Journal of Immunology,14 pgs. PMID: 27233958.

21. Chen, Qing, et al. (2016). Carcinoma-astrocyte gap junctions promote brain metastasis by cGAMP transfer. Nature. PMID: 27225120.

22. Borhis, Gwenoline, et al. (2016). Plasmacytoid dendritic cells and myeloid cells differently contribute to B-cell-activating factor belonging to the tumor necrosis factor superfamily overexpression during primary HIV infection. Wolters Kluwer.  PMID: 26558721.

23. Deymier, Martin, et al. (2015). Heterosexual Transmission of Subtype C HIV-1 Selects Consensus-Like Variants without Increased Replicative Capacity or Interferon-α Resistance. PLOS Pathogens, 22 pgs. PMID: 26378795.

24. Jang, Mihue, et al. (2015). Design of a Platform Technology for Systemic Delivery of siRNA to Tumours Using Rolling Circle Transcription. Nature Communications,12 pgs. PMID: 26246279.

25. Han, Tao, et al. (2015). Set7 facilitates hepatitis C virus replication via enzymatic activity-dependent attenuation of the IFN-related pathway. Journal of Immunology,13 pgs. PMID: 25681344.

26. Wu, Zeguang (2015). Natural killer cells can inhibit the transmission of human cytomegalovirus in cell culture by using mechanisms from innate and adaptive immune responses. JVI, 12 pgs. PMID: 25540381.

27. Wolstein, Orit, et al. (2014). Preclinical Safety and Efficacy of an Anti-HIV-1 Lentiviral Vector Containing a Short Hairpin RNA to CCR5 and the C46 Fusion Inhibitor. The American Society of Gene & Cell Therapy,14 pgs. PMID: 26015947.

28. Hsu, Yu-Lin, et al. (2014). Up-Regulation of Galectin-9 Induces Cell Migration in Human Dendritic Cells Infected with Dengue Virus. Journal of Cellular and Molecular Medicine,12 pgs. PMID: 25754930.

29. Drewes, Julia, et al. (2014). Attenuation of Pathogenic Immune Responses During Infection with Human and Simian Immunodeficiency Virus (HIV/SIV) by the Tetracycline Derivative Minocycline. PLOS One,15 pgs. PMID: 24732038.

30. Cai, Xinming, et al. (2014). PIKfyve, a class III lipid kinase, is required for TLR-induced type I IFN production via modulation of ATF3. Journal of Immunology,8 pgs. PMID: 24600036.

31. Benedict, et al. (2014). Use of monitoring levels of soluble forms of cytokeratin 18 in the urine of patients with superficial bladder cancer following intravesical Ad-IFNα/Syn3 treatment in a phase l study. Nature, 4 pgs. PMID: 24503570.

32. Leng, Qixin, et al. (2012). Buffering capacity and size of siRNA polyplexes influence cytokine levels. American Society of Gene & Cell Therapy, 9 pgs. PMID: 23032972.

33. Shukla, Nikunj M, et al. (2012). Potent adjuvanticity of a pure TLR7-agonistic imidazoquinoline dendrimer. PLOS One, 11 pgs. PMID: 22952720.

34. Osterlund, Pamela, et al. (2012). Incoming influenza A virus evades early host recognition, while influenza B virus induces interferon expression directly upon entry. JVI,11 pgs. PMID: 22855501.

35. Hayes, Melissa, et al. (2012). Pathogenic Old World Arenaviruses Inhibit TLR2/Mal-Dependent Proinflammatory Cytokines In Vitro. JVI,12 pgs. PMID: 22532679.

36. Haseley, et al. (2012). Extracellular Matrix Protein CCN1 Limits Oncolytic Efficacy in Glioma. Cancer Research, 9 pgs. PMID: 22282654.

37. Zhu, Rui-Yu, et al. (2011). Quantitative detection of fusion protein rIFN-β-HSA by a sandwich ELISA. African Journal of Biotechnology, 7 pgs. PMID: no PMID.

38. Dzopalic, Tanja, et al. (2010). Loxoribine, a selective Toll-like receptor 7 agonist, induces maturation of human monocyte-derived dendritic cells and stimulates their Th-1- and Th-17-polarizing capability.International Imunopharmacology, 6 pgs. PMID: 20817120.

39. Tassone, Laura, et al. (2010). Defect of Plasmacytoid Dendritic Cells in Warts, Hypogammaglobulinemia, Infections, Myelokathexis (WHIM) Syndrome Patients. Blood Journal,5 pgs. PMID: 20736454.

40. Golding, Amit, et al. (2010). Interferon-alpha Regulates the Dynamic Balance Between Human Activated Regulatory and Effector T Cells: Implications for Antiviral and Autoimmune Responses. Immunology, 11 pgs. PMID: 20465564.

41. Liang, Xueqing, et al (2010). Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.. Blood Journal, pgs. PMID: 20339095.

42. Takahashi, Ken, et al. (2010). Plasmacytoid dendritic cells sense hepatitis C virus–infected cells, produce interferon, and inhibit infection. PNAS, 6 pgs. PMID: 20231459.

43. Chavez, Deborah, et al. (2009). Antiviral activity and host gene induction by tamarin and marmoset interferon-α and interferon-γ in the GBV-B primary hepatocyte culture model. Virology, 11 pgs. PMID: 19501869.

44. Chase, Jennifer, et al. (2009). Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4. Infection and Immunity, 16 pgs. PMID: 18981246.

45. Calcedo, Roberto, et al. (2008). Host Immune Responses to Chronic Adenovirus Infections in Human and Nonhuman Primates. JVI, 10 pgs. PMID: 19116257.

46. Hooks, John, et al. (2008). IFN-beta provides immuno-protection in the retina by inhibiting ICAM-1 and CXCL9 in retinal pigment epithelial cells. Journal of Immunology, 9 pgs. PMID: 18322185.

47. Xu, Jianfeng, et al. (2007). High-yields and extended serum half-life of human interferon alpha2b expressed in tobacco cells as arabinogalactan-protein fusions. Biotechnology and Bioengineering, 13 pgs. PMID: 17328066.

48. Xu, Dongsheng, et al. (2006). The Latent Membrane Protein 1 of Epstein-Barr Virus (EBV) Primes EBV Latency Cells for Type I Interferon Production. JBC, 7 pgs. PMID: 16469740.

49. Francois, Catherine, et al. (2005). Quantification of Different Human Alpha Interferon Subtypes and Pegylated Interferon Activities by Measuring MxA Promoter Activation. Antimicrobial Agents and Chemotherapy,6 pgs. PMID:

50. Kuchtey, John, et al. (2005). Enhancement of dendritic cell antigen cross-presentation by CpG DNA involves type I IFN and stabilization of class I MHC mRNA. Journal of Immunology, 11 pgs. PMID: 16081792.

51. Hartmann, Gunther, et al. (2005). CpG oligonucleotides induce strong humoral but only weak CD4+ T cell responses to protein antigens in rhesus macaques in vivo. Vaccine, 7 pgs. PMID: 15837237.

52. Schmidt, Kerstin N, et al. (2004). APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNA. Journal of Immunology, 7 pgs. PMID: 14688319.

53. Qu, Chunfeng, et al. (2003). Autocrine type I IFN and contact with endothelium promote the presentation of influenza A virus by monocyte-derived APC. Journal of Immunology,10 pgs. PMID: 12517968.

54. Osborn, Blaire, et al. (2002). Pharmacokinetic and pharmacodynamic studies of a human serum albumin-interferon-alpha fusion protein in cynomolgus monkeys. The Journal of Pharmacology and Experimental Therapeutics,9 pgs. PMID: 12388634.

55. Keir, Mary, et al. (2002). IFN-alpha secretion by type 2 predendritic cells up-regulates MHC class I in the HIV-1-infected thymus. Journal of Immunology,8 pgs. PMID: 11751977.

看到这儿，您心动了吗？马上联系小艾吧！ 美国的PBL Assay Science（又名：Pestka Biomedical Laboratories, Inc.）成立于1990年，创始人Sidney Pestka被称为“干扰素之父”，PBL Assay Science 作为干扰素和细胞因子蛋白和抗体以及预包装的一流的干扰素和细胞因子免疫测定试剂盒的高质量制造商而享有盛誉。生产和销售的高品质干扰素产品和生物标志物检测试剂盒在很多高影响力出版物中都有引用，并已在具有挑战性的样本中进行了外部验证。 

PBL Assay Science提供各种干扰素亚型的蛋白，抗体和检测试剂盒，如：IFN-Alpha（IFN-ɑ 2a，IFN-ɑ 2b，IFN-ɑ 5，IFN-ɑ 6，IFN-ɑ 7，IFN-ɑ 14，IFN-ɑ 16，IFN-ɑ 17......），IFN-Beta（IFN-β 1a，IFN-β 1b），IFN-Lambda（IFN-λ），IFN-Omega（IFN-ω），IFN-Gamma（IFN-γ）...... 

武汉艾美捷科技有限公司，简称艾美捷，拥有进出口资质和自己的国际物流，与国内外90余家知名的试剂、原料及技术服务供应商深度合作，是国内生物制药企业、诊断企业、跨国药企、各大高校院所采购平台的首选定供应商。