一、植物和藻类内参抗体
选择合适的植物内参,首先考虑样本来源,植物样本需要选用植物细胞表达稳定的蛋白比如Actin或者Rubisco作为内参;其次要考虑目的蛋白的分子量大小,我们推荐内参要与检测的目的蛋白的分子量相差5kD以上,以区分目的蛋白条带;最后,考虑目的蛋白的表达定位,选择对应的内参抗体,比如,全细胞、细胞核、线粒体等等。同时,在特定条件下,内参蛋白的表达会出现异常,应该将其考虑在内。
在此,我们为您推荐Agrisera植物内参抗体。优势是适用于植物和藻类,而且在很多种属类型中得到验证,发表过很多文献,应用范围广泛。Agrisera 公司提供的植物领域的抗体涵盖拟南芥、莱茵衣藻、蓝藻、松柏类、硅藻、大豆、大麦、蒺藜苜蓿、烟草、稻、菜豆、小立碗藓、杨树、豌豆、番茄、马铃薯、菠菜、小麦、玉米、葡萄等多个物种,此外还有水生生物类(藻类、鱼)抗体,细菌、昆虫、真菌抗体。更多详情点击>
二、Rubisco抗体
Rubisco(核酮糖-1,5-二磷酸羧化酶/加氧酶)催化光合生物中CO2固定的限速步骤。从紫色细菌到植物,它的同源性已经得到了证实,并且由两个蛋白质亚基组成,每个亚基有8个拷贝。在植物和绿藻中,大亚基(~55kDa)由叶绿体rbcL基因编码,小亚基(15kDa)由核rbcS基因家族编码。更多详情点击>
产品推介:
产品名称 | 产品货号 | 应用类型 | 产品说明 |
ACT | Actin | AS13 2640 | IF, WB | 详情 |
Actin-11 | AS10 702 | IF, WB | 详情 |
Tubulin alpha chain | AS10 680 | IF, WB | 详情 |
Tubulin beta chain | AS10 681 | IF, WB | 详情 |
H3 | Histone H3 (rabbit antibody) (nuclear marker) | AS10 710 | ChIp-qPCR, IF, WB | 详情 |
AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit) | AS05 085 | Blue Native-PAGE, IF, WB | 详情 |
AtpB | Beta subunit of ATP synthase, mitochondrial | AS16 3976 | WB | 详情 |
RA | Rubisco activase | AS10 700 | WB | 详情 |
RbcL | Rubisco large subunit,form I | AS01 017 | WB,免疫定位,免疫胶体金 | 详情 |
RbcL | Rubisco large subunit,form I and form II | AS03 037 | WB,IF,免疫胶体金,组织印记 | 详情 |
RPN6 | 26S proteasome non-ATPase regulatory subunit 9 | AS15 2832A | WB | 详情 |
用于检测和定量广泛的植物和藻类物种中的Rubisco,发表多篇文献。
产品名称 | 产品货号 | 产品说明 |
RbcL | Rubisco large subunit, form I and form II | AS03 037 | 详情 |
RbcL | Rubisco large subunit, form I and form II (affinity purified) | AS03 037A | 详情 |
RbcL | Rubisco large subunit, form I and form II, HRP-conjugated (40 ?g) | AS03 037-HRP | 详情 |
Rubisco large subunit, pre-immune serum | AS03 037PRE | 详情 |
RbcL | Rubisco large subunit, form I and form II, Biotin conjugated (40 ?g) | AS03 037B | 详情 |
RbcL | Rubisco large subunit, form I and form II, ALP-conjugated (40 ?g) | AS03 037-ALP | 详情 |
***货号AS03 037是Agrisera公司产品发表文章最多的一款“明星产品”,是植物和藻类内参抗体的首选。
Agrisera植物内参抗体发表文章:
1. Overexpressing the H﹑rotein of the glycine cleavage system increases biomass yield in glasshouse and fieldゞrown transgenic tobacco plants. Plant Biotechnology Journal. 2018.
2. Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. PLoS One. 2018.
3. The Arabidopsis transcription factor bZIP11 activates auxin-mediated transcription by recruiting the histone acetylation machinery. Nature Communications.2014
4. Co-localization of P-glycerate kinase, P-ribulokinase, ADP-glucose pyrophosphorylase and Rubisco activase with CF1 in pea leaf chloroplasts. Plant Science. 2009
5. Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Scientific Reports. 2017
6. Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface. Nature Communications.2014
7. Tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperatures. Scientific Reports. 2016
上一篇:植物环境压力
下一篇:Duchefa植物培养基
微信扫码在线客服