WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human SKP2 (Isoform SKP2_alpha) has a predicted length of 424 residues and MW of 48 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (HRKHLQEIPDLSSNVATSF) corresponding to a region (2-20) from the N-terminus of human S-phase kinase-associated protein 2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.