This kit provides a fast, reliable and convenient method to purify and concentrate using a two column system for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from fresh or frozen plasma/serum sample volumes ranging from 5 mL- 10 mL.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL. All components for the purification & concentration are provided in one convenient & fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here.The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis, microarrays and NGS for various analyses including Fetal DNA screening, genotyping and other oncology and autoimmune disease research.
产品特点
Efficient purification of DNA from large plasma and serum input volumes (5-10 mL); Rapid and simple purification of all sizes of circulating DNA from plasma and serum samples; Two kits in one - purify and concentrate DNA from bodily fluids using a convenient two column system; Concentrate circulating DNA into a flexible elution volume ranging from 25 µL - 50 µL; Isolate inhibitor-free cell-free circulating DNA; Compatible with Streck Cell-Free DNA BCT® Tubes.
