This kit provides a fast, reliable and convenient method to purify and concentrate using a two column method for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from fresh or frozen plasma/serum sample volumes ranging from 1 mL - 4 mL.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL. All components for the purification & concentration are provided in one convenient & fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here.The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS, for various analyses: Fetal DNA screening, genotyping, and other oncology and autoimmune diseases research.
产品特点
Isolate all sizes of circulating DNA from plasma and serum samples; Isolate viral and bacterial DNA; Versatile plasma and serum input volumes (1 mL – 4 mL); Concentrate circulating DNA into a flexible elution volume ranging from (25 µL - 50 µL); Isolate inhibitor-free cell-free circulating DNA; Purify high-quality DNA in 90 minutes; Compatible with Streck Cell-Free DNA BCT® Tubes.
