英文名字 Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit (Slurry Format)
中文名称 血浆/血清循环核酸纯化大提试剂盒(提取试剂盒)
供应商 Norgen Biotek
产品货号 NGB-53400
产品报价 6867/25Preps
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产品描述 Norgen's Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides a fast, reliable and simple procedure for isolating circulating nucleic acids (mRNA, miRNA, exosomal RNA, viral RNA, DNA and viral DNA) from various amounts of plasma/serum ranging from 2 mL to 5 mL.This kit is able to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA and circulating DNA. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating NA from large sample volumes. Free-Circulating NA can be isolated from either fresh or frozen samples using this kit, and the kit allows for the concentration of NA that is present in low concentrations (1-100ng/mL in human plasma). Typical yields of free-circulating NA will vary depending on the input sample, as the amount of NA present in plasma and serum will depend upon the health status of the individual and the level of nucleases present in the blood. This kit is suitable for the purification of Nucleic Acid from serum or plasma prepared from blood collected on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.Free-circulating nucleic acids in plasma and serum have the potential to provide biomarkers for certain cancers and disease states, and include tumor-specific extracellular nucleic acid fragments and fetal DNA in maternal blood. Free-circulating DNA is usually present as short fragments of less than 1000 bp, whereas free-circulating RNA and exosomal RNA in plasma and serum are usually present as short fragments of less than 1000 nt. In addition, plasma or serum also contains free-circulating miRNA that are as small as 21 nt. Furthermore, circulating viral DNA or viral RNA may be present in the plasma or the serum of viral-infected patients. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides an efficient method for the purification of all sizes of these fragmented free-circulating nucleic acids from human plasma or serum.
产品特点 Isolate all sizes of circulating and exosomal RNA, including microRNA; Isolate all sizes of circulating DNA from plasma and serum samples; Versatile plasma and serum input volumes (2 mL to 5 mL); Concentrate circulating nucleic acids into small elution volumes; Isolate inhibitor-free circulating nucleic acids; Bind and elute all RNA irrespective of size or GC content, without bias.
保存建议 所有缓冲液应密封保存,并在室温(15-25°C)下保存长达1年。具体保存情况收到产品后请按说明书存放。
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其他 Norgen Biotek目前的产品多达700余种,提供一流的样品制备试剂盒,应用于基因组和蛋白组等不同的领域。拥有超过二十个专利,作为一家成长型的公司,其产品主要覆盖包括各种标本类型的DNA,RNA和蛋白质的纯化富集,从活检样本(包括尿液,血浆,血清和血液)中纯化RNA和DNA,全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。此外,Norgen Biotek最近推出了100多款用于核酸诊断的试剂盒,包括从尿液、血浆和血清中诊断人类病原体的试剂盒,诊断食源性病原体的试剂盒和诊断植物病原体的试剂盒。这些试剂盒仅供研究使用,非常适合用于监测耐药病原体,流行病学研究,病原体现场监测和调查。Norgen Biotek还致力于为科研和临床试验提供高质量的分子诊断测试。

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