FC; IC; IH, frozen; WB; IP; Immunopanning Flow Cytometry: 5-20 µg/mL. Western Blotting: 0.5-2.0 µg/mL, non-reducing conditions only (no DTT or beta-mercaptoethanol). Immunoprecipitation: lysate dependent. 10 ug per 200-500 ug total protein. Immunopanning: 1-5 µg/mL. Immunocytochemistry: 1-5 µg/mL. Staining is strongest in non-fixed cells, light fixation is tolerable. Immunohistochemistry: fresh, acetone fixed sections only, epitope is fixation sensitive. Not suitable in formalin-fixed, paraffin (FFPE) embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.