WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 4.0 µg/mL is recommended for WB. Human G1/S-specific cyclin-D1 has a predicted length of 295 residues and MW of 34 kDa. A concentration of 8.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.