EDN1 is a powerful mitogen mediator of smooth muscle tone， and inflammatory regulator that is involved in diseases of the airways， pulmonary circulation， and inflammatory lung diseases， both acute and chronic. Endothelin-2 and Endothelin-3 vary from Endothelin-1 by two and six amino acids， respectively， and share homology， particularly at the carboxy terminus with sarafotoxins a-e. The lung has the largest levels of EDN1 secreted by endothelium， smooth muscle， airway epithelium， and a diversity of other cells. ET-1 also circulates in the plasma. Endothelin-1 along with endothelin-2， & endothelin-3 （ET-3） consist of the endothelin family of 21 amino acid peptides manufactured in a variety of cells and tissues， particularly endothelial and epithelial lineages. These endothelins are derivatives from three genes. The endothelins are normally connected with hypertension， though they regulate a diversity of other effects through two G-protein coupled receptors， ET-A and ET-B. The endothelins are derivatives from a precursor peptides using cleavage by metalloproteases for instance endothelin-converting enzymes.
Lyophilized EDN1 although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution EDN1 should be stored at 4℃ between 2-7 days and for future use below
-18℃.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
