- 中文名称
5-HIAA, Human (5-hydroxy-3-indole Acetic Acid) BioAssay ELISA Kit
- 英文名字
- 5-HIAA, Human (5-hydroxy-3-indole Acetic Acid) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- H3650-50
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The primary carcinoid tumor is usually derived from the enterochromaffin cells of the midgut and is located most frequently in the terminal ileum. Carcinoid tumors generally secrete various amounts of indoles. The carcinoid syndrome is generally characterized by an increased urinary excretion of 5-hydroxy-3-indole acetic acid (5-HIAA), the end product of serotonin (5-HT) metabolism. Traditionally, 5-hydroxy-3-indole acetic acid is assayed by diazotization with nitrosonaphtol to form a purple color. However, it is well documented that many other substances present in the urine interfere with this reaction to give false-positive results. Attempts were made to overcome this problem by a combination of ion exchange chromatography and fluorometry. These methods, however, lack sensitivity and are time consuming. Recently, high performance liquid chromatographic analyses of 5-HIAA with fluorometry in the ultraviolet region of the spectrum or electrochemical detection have been described. Both methods require solvent extraction because of the numerous interfering compounds present in urine. The 5-HIAA enzyme immunoassay is a new and simple method for the quantification of this important marker of carcinoid syndrome in small urine samples.Sample Type: UrineIntended Use: Enzyme immunoassay for the quantitative determination of 5-HIAA in human urine.Test Principle:The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards. Kit Components:Microtiter Plate 1x96 wells5-HIAA Pab 1x5ml5-HIAA (Biotin) 1x5mlBiotin Polyclonal antibody (AP) (100X)1x0.2mlStandard 0mg/L 1x500ulStandard 0.4mg/L 1x500ul Standard 1.0mg/L 1x500ulStandard 2.75mg/L 1x500ulStandard 7.5mg/L 1x500ulStandard 20mg/L 1x500ulStandard 55mg/L 1x500ulControl 1 normal, 1x1vialControl 2 pathological , 1x1vialMethylation Reagent 1x2ml HCl Solution, 1x1ml, 0.1M HCl.Assay Buffer, Concentrate, 10X 1x50mlDilution Reagent 1x4mlWash Buffer, Concentrate, 20x, 1x50mlPNPP Substrate, 9x1Tablets Substrate Buffer 1x27mlStop Solution 1x5mlStorage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对5-HIAA, Human (5-hydroxy-3-indole Acetic Acid) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到5-HIAA, Human (5-hydroxy-3-indole Acetic Acid) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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