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中文名称

Glutathione Detection BioAssay Kit, Colorimetric (GSH)

英文名字
Glutathione Detection BioAssay Kit, Colorimetric (GSH)
供应商
USBiological
产品货号
G8124-05
产品报价
¥1/100Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Glutathione (GSH) is the major intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative stress in mammalian cells. Glutathione Detection BioAssay Kit (GSH) provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader. The assay is based on the glutathione recycling system by DTNB and glutathione reductase. DTNB and glutathione (GSH) react to generate 2-nitro-5-thiobenzoic acid which has yellow color. Therefore, GSH concentration can be determined by measuring absorbance at 412nm. The generated GSSG can be reduced back to GSH by glutathione reductase, and GSH reacts with DTNB again to produce more 2-nitro-5-thiobenzoic acid. Therefore, the recycling system dramatically improves the sensitivity of total glutathione detection. The kit includes the 5-Sulfosalicylic acid (SSA) for the removal of proteins from samples and for the protection of GSH oxidation and gamma-glutamyl transpeptidase reaction. The kit can quantify glutathione from 1-100ng/well in a 200ul reaction. For detecting lower glutathione concentrations, such as in blood samples, increasing reaction time will generate stronger signal. The kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture. The sensitivity for detecting the reduced form of glutathione (without recycling system) is 100 times lower than detecting the total glutahione.Kit Components:100 assays Cap colorGlutathione Reaction Buffer 100ml NMGlutathione Substrate (DTNB) 2 vials RedNADPH Generating Mix (lyophilized) 2 vials BlueGlutathione Reductase (lyophilized) 2 vials GreenSulfosalicylic Acid (SSA, 1 gram) 1 bottle WMGSH Standard (lyophilized, MW 307) 2 x 1mg Yellow
产品特点
针对Glutathione Detection BioAssay Kit, Colorimetric (GSH)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Glutathione Detection BioAssay Kit, Colorimetric (GSH)产品后将其置于-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
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