- 中文名称
Glutathione, Colorimetric Assay Kit, BioAssay
- 英文名字
- Glutathione, Colorimetric Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- G8123-50
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Glutathione (gamma-glutamylcysteinylglycine or GSH) is a naturally occuring tripeptide whose nucleophilic and reducing properties play a central role in metabolic pathways, as well as in the antioxidant system of most aerobic cells.1 GSH plays a critical role as a coenzyme with a variety of enzymes including, glutathione peroxidase, glutathione S-transferase and thiol transferase. GSH also plays major roles in drug metabolism, calcium metabolism, the gamma-glutamyl cycle, blood platelet and membrane functions. In addition, GSH is crucial to a variety of life processes, including the detoxification of xenobiotics, maintenance of the -SH level of proteins, thiol-disulfide exchange, removal of hydroperoxides and free radicals, and amino acid transport across membranes. Physiological values of intracellular GSH generally range from 1 to 10mM. Although many methods have been described for the assay of GSH, the reliable ones are labor intensive and not easy to use.Principle of the Assay:This colorimetric method for GSH quantitation is based on a chemical reaction, which proceeds in two steps. The first step leads to the formation of substitution products (thioethers) between a patented reagent, R1 (4-chloro-1-methyl-7-trifluromethyl-quinolinium methylsulfate), and all mercaptans (RSH) which are present in the sample.The second step is a beta-elimination reaction which takes place under alkaline conditions. This reaction is mediated by reagent R2 (30% NaOH) which specifically transforms the substitution product (thioether) obtained with GSH into a chromophoric thione which has a maximal absorbance wavelength at 400nm.This method makes it possible to specifically assay glutathione with only one sampling and one colorimetric measurement. A modification of this method can be used to assay other mercaptans. This is based on the measurement of substitution products (thioethers) which absorb light at 356nm in the absence of reagent R2.Because of its simplicity, this method is especially well adapted to the assay of glutathione in large series of biological samples. The main advantage of the method is that it is specific for glutathione and it does not require use of an enzyme as a reagent.Kit Components:100 assays1. G8123-50A: Reagent (R1) 1x5.5ml2. G8123-50B: 30% NaOH (R2) 1x10ml3. G8123-50C: Buffer (solution 3) 1x100mlThese solutions are ready for use.Storage and Stability:Reagent bottles should always be kept tightly closed and stored at 4°C. For each experiment, take out the required amount of buffer and reagents. Transfer should be made using clean pipette tips to avoid contamination. Immediately close the remaining buffer and reagent bottles and store them at 4°C. Do not leave reagent bottles open on the bench, at room temperature or exposed to light. Under the above conditions, all reagents are stable for 12 months.
- 产品特点
- 针对Glutathione, Colorimetric Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glutathione, Colorimetric Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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