- 中文名称
Glutathione (GSH/GSSG/Total) Assay Kit, BioAssay
- 英文名字
- Glutathione (GSH/GSSG/Total) Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- G8123-40
- 产品报价
- ￥1/100Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Glutathione is the major intracellular low molecular weight thiol that plays a critical role in cellular defense against oxidative stress in tissues and cells. Commercially available glutathione detection kits, such as the DTNB-enzyme cycling glutathione assay kit or the Monochlorobimane based assay kit hardly distinguish between reduced glutathione (GSH; FW: 307) and oxidized glutathione (GSSG; FW: 612). This Glutathione Detection Kit provides a unique, convenient tool for detecting GSH, GSSG, and total glutathione individually. In the assay, OPA reacts with GSH (not GSSG), generating fluorescence, so GSH can be specifically quantified. Adding a reducing agent converts GSSG to GSH, so (GSH + GSSG) can be determined. To measure GSSG specifically, a GSH Quencher is added to remove GSH, preventing reaction with OPA (while GSSG is unaffected). Reducing agent is then added to destroy excess quencher and to convert GSSG to GSH. Thus, GSSG can be specifically quantified. The kit provides a unique procedure and buffer formula to eliminate protein thiol interference and to stabilize GSH and GSSG in solution. The assay is easy to perform and detects 2-400ng/ul of GSH, GSSG or total glutathione.Kit Components:G8123-40A: Glutathione Assay Buffer, 1x30mlG8123-40B: PCA (Perchloric Acid, 6N), 1x2mlG8123-40C: KOH (3N), 1x2ml*G8123-40D: OPA Probe (o-phthalaldehyde), 1x200ul*G8123-40E: Reducing Agent Mix, x1vial*G8123-40F: GSH Quencher 1x20ul*G8123-40G: GSH Standard (FW: 307), 1x1mgStorage and Stability:Store components at -20°C. Kit is stable for 6 months after receipt. Reconstituted *G8123-40D, *G8123-40E, *G8123-40F, *G8123-40G are stable for 2 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Sample Collection and Storage:Tissue and Cell Samples:GSH is labile and cell preparations will oxidize it rapidly. Keep all samples and reagents ice cold and work as rapidly as possible. Prepare centrifuge tubes with 20ul PCA on ice to receive samples. Homogenize 2-4 x10e6 cells or 40mg tissue on ice with 100ul of ice cold Glutathione Assay Buffer. Take 60ul of each homogenate to a prechilled tube containing PCA and vortex several seconds to achieve a uniform emulsion. Keep on ice for 5 min. Spin 2 min at 13,000xg at 4°C, collect supernatant (containing glutathione) and discard the protein pellet. The sample can then be stored at –70°C and is stable for a month. Serum or Other Liquid Samples:Freeze samples immediately upon acquisition and keep frozen until ready for processing. Take 60ul thawed sample to centrifuge tube containing 20ul ice cold PCA, vortex and keep on ice for 5 min. Spin for 2 min at 13,000xg at 4°C. Collect the supernatant. The sample can then be stored at –70°C and is stable for a month.
- 产品特点
- 针对Glutathione (GSH/GSSG/Total) Assay Kit, BioAssay 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glutathione (GSH/GSSG/Total) Assay Kit, BioAssay 产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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