- 中文名称
Glutamate Assay Kit, BioAssay
- 英文名字
- Glutamate Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- G3990-11
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Glutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism and Alzheimer’s disease. Glutamate is also widely used as a flavor enhancer in the food industry.Simple, direct and automation-ready procedures for measuring glutamate concentration are very desirable. Glutamate assay kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample.Applications:Direct Assays: glutamate in serum, plasma, tissue extracts and food extract samples.Drug Discovery/Pharmacology: effects of drugs on glutamate levels.Key Features:Sensitive and accurate. Detection limit of 50uM, linearity up to 2.5mM glutamate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96- well plate assay for thousands of samples per day.Kit Contents:(100 tests in 96-well plates) Assay Buffer: 10mlNAD Solution:1ml Enzyme Mix: 120ulMTT Solution: 1.5ml Standard: 1ml 100mM GlutamateStorage conditions. Store all reagents at -20°C. Shelf life: 6 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:1. Calibration Curve. Prepare 600ul 2.5mM Glutamate Premix by mixing 15ul 100mM Standard and 585ul distilled water. Dilute standard as follows. Transfer 20ul standards into wells of a clear bottom 96-well plate.No Premix+H2O Vol (ul) Glutamate (mM)1 100ul+0ul 100 2.52 80ul+20ul 100 2.03 60ul+40ul 100 1.54 40ul+60ul 100 1.05 30ul+70ul 100 0.756 20ul+80ul 100 0.57 10ul+90ul 100 0.258 0ul+100ul 100 0Samples: add 20ul sample per well in separate wells.IMPORTANT: Serum and tissue extract samples require a sample blank.2. Reagent Preparation. Spin the Enzyme Mix tube briefly before pipetting. For each well of reaction, prepare Working Reagent by mixing 60ul Assay Buffer, 1ul Enzyme Mix, 5ul NAD and 14ul MTT. Fresh reconstitution is recommended. Where a sample blank in required, prepare a Blank Working Reagent by mixing 60ul Assay Buffer, 5ul NAD and 14ul MTT (i.e. No Enzyme Mix).3. Reaction. Add 80ul Working Reagent (or Blank Working Reagent where appropriate) per reaction well quickly. Tap plate to mix briefly and thoroughly.4. Read optical density (OD0) for time “zero” at 565 nm (520-600nm) and OD30 after a 30-min incubation at room temperature.5. Calculation:. Subtract OD0 from OD30 for the standard and sample wells. Next, subtract the DODwater (Std 8) from each DODstandard and DODsample to obtain the DDODs. (Where a sample blank was required, subtract the DODblank from DODsample to obtain the DDODsample.) Plot the DDODstandard’s and use this standard curve to convert the DDODsample values to sample glutamate concentration.[Glutamate] =DDODSAMPLESlope (mM)Note: If the sample DDOD values are higher than the DDOD value for the 2.5mM glutamate standard, dilute sample in distilled water and repeat this assay. Multiply the results by the dilution factor.Conversions: 1mM glutamate=14.5 mg/dL.Materials Required, But Not Provided:Pipeting (multi-channel) devices. Clear-bottom 96-well plates (e.g. Corning Costar) and plate reader.General Considerations:1. This assay is based on an enzyme-catalyzed kinetic reaction. Addition of Working Reagent should be quick and mixing should be brief but thorough. Use of multi-channel pipettor is recommended.2. The following substances interfere and should be avoided in sample preparation: EDTA (>0.5mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).
- 产品特点
- 针对Glutamate Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glutamate Assay Kit, BioAssay产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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