- 中文名称
a-Glucosidase Assay Kit, BioAssay
- 英文名字
- a-Glucosidase Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- G3058-11
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- a-Glucosidase hydrolyzes the terminal, non-reducing 1,4-linked a-Dglucose residues with release of a-D-glucose. a-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring a- glucosidase activity are becoming popular in Research and Drug Discovery. a-Glucosidase Assay Kit is designed to measure a-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-a-Dglucopyranoside that is hydrolyzed specifically by a-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.Key Features:High sensitivity and wide linear range. Use 20ul sample. The detection limit is 2 U/L, linear up to 250 U/L.Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of a-glucosidase activity within 20 minutes.Robust and amenable to high throughput systems. All reagents are compatible with highthroughput liquid handling instruments.Applications:Direct Assays: a-glucosidase activity in biological samples.Characterization and Quality Control for a-glucosidase production.Drug Discovery: high-throughput screen and evaluation of a-glucosidase inhibitors.Kit Contents: (100 tests in 96-well plates)Assay Buffer, pH 7.0, 1x24ml a-NPG Substrate, 1x1mlCalibratorl (equivalent to 250 U/L), 1x10mlStorage and Stability:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Materials Required, But Not Provided:Pipeting devices and accessories (e.g. multi-channel pipettor) Clear bottom 96-well plates (e.g. Corning Costar) plate readerProcedures:This assay is based on a kinetic reaction. Use of a multi-channel pipettor is recommended. Addition of Working Reagent to samples should be quick and mixing should be brief but thorough. Assays can be executed at room temperature or 37°C.Reagent preparation: equilibrate reagents to room temperature. The Working Reagent is prepared by mixing for each 96-well assay, 200ul Assay Buffer and 8ul a-NPG substrate (final 1.0mM). Fresh reconstitution is recommended, although the Working Solution is stable for at least one day at room temperature.Sample preparation: enzyme samples can be in 50mM phosphate buffer, pH 7.0 or in any other suitable enzyme buffer. The following chemicals are known to affect the enzyme activity and should be avoided. SH-containing reagents (e.g. dithiothreitol, 2-mercaptoethanol, glutathione), Ca2+, Cu2+, Fe3+/Fe2+, Hg2+, Mg2+, Ni2+, Zn2+, SDS, Triton X-100, Tween, digitonin, EDTA and Tris.Procedure using 96-well plate:1. Transfer 20ul distilled water (dH2O) to two wells of a clear bottom 96-well plate. Add 200ul dH2O to one of these wells and 200ul Calibrator to the other well (total volume 220ul).Transfer 20ul samples into other wells. Transfer 200ul Working Reagent to the sample wells only. The final reaction volume in the sample wells is 220ul. Tap plate briefly to mix.2. Read OD405nm (t=0), and again after 20 min (t=20 min) on a plate reader.3. Calculation: a-glucosidase activity of the sample (U/L) isa-Glucosidase Activity = OD20 - OD0x 250 (U/L)OD(Calibrator) - OH(H2O)OD20 and OD0 are OD405nm values of sample at 20 and 0 min, respectively. OD(calibrator) and OD(H2O) are OD405nm values of Calibrator and H2O at 20 min.Unit definition: one unit of enzyme catalyzes the hydrolysis of 1uMole of substrate per min at pH 7.0.
- 产品特点
- 针对a-Glucosidase Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到a-Glucosidase Assay Kit, BioAssay产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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