- 中文名称
Gas6, Mouse, BioAssay ELISA Kit (Growth Arrest-specific Protein 6, GAS-6, AXL Receptor Tyrosine Kinase Ligand)
- 英文名字
- Gas6, Mouse, BioAssay ELISA Kit (Growth Arrest-specific Protein 6, GAS-6, AXL Receptor Tyrosine Kinase Ligand)
- 供应商
- USBiological
- 产品货号
- G2016-06
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Gas6 is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest. It is expressed by endothelial cells, fibroblasts, neurons, smooth muscle cells, and platelets and plays a role in vascular, thrombotic, atherosclerotic, inflammatory, autoimmune, renal, and cancer pathologies. Secreted Gas6 is a 75kD, 647aa molecule. Both Gas6 and the related Protein S contain an extensively g-carboxylated N-terminal Gla domain, four EGF-like repeats, and two C-terminal laminin G-like domains that bear resemblance to sex hormone binding globulin (SHBG). In parallel with the structurally-related Protein S, Gas6 is dependent upon vitamin K for activity. Within the EGF-like and SHBG-like domains, mouse Gas6 shares 83%, 94% and 40% aa sequence identity with the equivalent regions in human Gas6, rat Gas6, and mouse Protein S, respectively. Gas6 binds to and induces signaling through the receptor tyrosine kinase TAM subfamily, which includes Axl, Dtk/Tyro3, and Mer. Shed (soluble) forms of Axl and Mer are known to bind Gas6 and function as decoy receptors. Gas6 has a number of divergent functions in tissue that are related to its ability to bind to multiple receptors. Through its Gla and laminin G domains, it participates in tissue homeostasis by 1) protecting cells from stress-induced apoptosis, an effect mediated by binding to Axl, and 2) promoting apoptotic cell phagocytosis by binding to Mer. In addition, it appears to have neuroprotective properties, an effect possibly mediated through Gas6 binding to either Tyro3 or Axl. Notably, the affinity of the g-carboxylated Gla domain for phosphatidylserine also likely contributes to its unusual role in phagocytosis as well as the cellular entry of select viruses. Gas6 may also function as a pro-inflammatory molecule by promoting platelet activation, the development of nephrotoxic nephritis, and atherosclerotic plaque instability. Conversely, it can also inhibit inflammatory cytokine production from monocytes/ macrophages and microglia. Gas6 is known to induce the proliferation of cardiac fibroblasts, Schwann cells, vascular smooth muscle cells, and NK cell precursors. It also inhibits VEGF-induced endothelial cell chemotaxis and can have either positive or negative effects on the proliferation and invasiveness of tumor cells. Circulating levels of Gas6 are elevated in a variety of conditions including venous thromboembolic disease, systemic lupus erythematosus, and systemic inflammatory responses. It is also present in the cerebrospinal fluid of patients with chronic inflammatory demyelinating polyneuropathy (CIDP).Sample Type:Cell culture supernates, cell lysates, mouse serum, and plasma.Intended Use:For the quantitative determination of mouse Growth Arrest Specific 6 (Gas6) concentrations in cell culture supernates, cell lysates, mouse serum, and plasma.Sensitivity:Forty-six assays were evaluated and the minimum detectable dose (MDD) of mouse Gas6 ranged from 0.004-0.027ng/ml. The mean MDD was 0.014ng/ml. The MDD was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.Specificity:Recognizes recombinant and natural mouse Gas6.Test Principle:This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse Gas6 has been pre-coated onto a microplate. Standards, Control, and samples are pipetted into the wells and any mouse Gas6 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for mouse Gas6 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells. The enzyme reaction yields a blue product that turns yellow when the Stop Solution is added. The intensity of the color measured is in proportion to the amount of mouse Gas6 bound in the initial step. The sample values are then read off the standard curve.Kit Components:Mouse Gas6 Microplate: 12 strips x 8 wellsMouse Gas6 Standard: 2x20ngMouse Gas6 Control (lyophilized): 2 vialsMouse Gas6 Conjugate: 12mlAssay Diluent RD1-43: 1x11mlCalibrator Diluent RD5-26 Concentrate: 1x21mlWash Buffer Concentrate: 1x50mlColor Reagent A: 1x12mlColor Reagent B: 1x12mlStop Solution: 1x23mlPlate Sealers: 4 adhesive stripsStorage and Stability:See Kit Protocol for detailed storage instructions.
- 产品特点
- 针对Gas6, Mouse, BioAssay ELISA Kit (Growth Arrest-specific Protein 6, GAS-6, AXL Receptor Tyrosine Kinase Ligand)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Gas6, Mouse, BioAssay ELISA Kit (Growth Arrest-specific Protein 6, GAS-6, AXL Receptor Tyrosine Kinase Ligand)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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