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细胞分析试剂盒
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中文名称

Galactose Assay Kit, BioAssay

英文名字
Galactose Assay Kit, BioAssay
供应商
USBiological
产品货号
G1025-13
产品报价
¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Galactose (C6H12O6) is a monosaccharide that is found in dairy products, sugar beets, gums and mucilages. It is also synthesized in mammals, where it forms part of glycolipids and glycoproteins in several tissues. It forms the disaccharide lactose when combined with glucose. Simple, direct and high-throughput assays for galactose determination find wide applications. Galactose Assay Kit uses specific enzyme-coupled reactions to form a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the galactose concentration in the sample.Key Features:Use as little as 20ul samples. Linear detection range in 96-well plate: 10 to 1000uM galactose for Colorimetric Assay:s and 10 to 100uM for Fluorometric Assay:s.Applications:Direct Assays: galactose in serum, plasma, urine, saliva, milk, culture medium and other biological samples. Drug Discovery/Pharmacology: effects of drugs on galactose metabolism.Food and Beverages: galactose in food and beverages products.Kit Contents:Assay Buffer: 10ml Enzyme Mix: 120ulDye Reagent: 120ul Standard: 1ml 10mM GalactoseStorage conditions. The kit is shipped on dry ice. Store all components at -20°C. Shelf life of three months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents.Please refer to Material Safety Data Sheet for detailed information.Colorimetric Procedure:Note: (1) glycerol and SH-containing reagents (e.g. b–mercaptoethanol, dithiothreitol) are known to interfere in this assay and should be avoided in sample preparation. (2) This assay is based on a kinetic reaction. To ensure identical incubation time, addition of Working Reagent to standard and samples should be quick and mixing should be brief but thorough. Use of a multi-channel pipettor is recommended. Sample treatment: serum and plasma samples can be assayed directly. Milk samples should be cleared by mixing 600ul milk with 100ul 6 N HCl. Centrifuge 5 min at 14,000 rpm. Transfer 300ul supernatant into a clean tube and neutralize with 50ul 6 N NaOH. The neutralized supernatant is ready for assay (dilution factor n=1.36).1. Equilibrate all components to room temperature. During experiment, keep thawed Enzyme Mix in a refrigerator or on ice.2. Standards and samples: prepare 400ul 1000uM Standard by mixing 40ul 10mM standard with 360ul dH2O. Dilute standard in dH2O as follows.No 1000uM STD+H2O Vol (ul) Galactose (uM)1 100ul+0ul 100 10002 80ul+20ul 100 8003 60ul+40ul 100 6004 40ul+60ul 100 4005 30ul+70ul 100 3006 20ul+80ul 100 2007 10ul+90ul 100 1008 0ul +100ul 100 0Transfer 20ul standards and 20ul samples into separate wells of a clear flat-bottom 96-well plate.3. Reaction. For each reaction well, mix 85ul Assay Buffer, 1ul Enzyme Mix (vortex briefly before pipetting), and 1ul Dye Reagent in a clean tube. Transfer 80ul Working Reagent into each reaction well. Tap plate to mix. Incubate 20 min at room temperature.4. Read optical density at 570nm (550-585nm).Fluorometric Procedure:For Fluorometric Assays, the linear detection range is 10 to 100uM galactose. Prepare 100uM galactose standard by mixing 10ul 10mM standard with 990ul H2O. Then dilute standards in H2O (see Colorimetric Procedure:) to 100, 80, 60, 40, 30, 20, 10 and 0uM.1. Transfer 20ul standards and 20ul samples into separate wells of a black 96-well plate.2. Add 80ul Working Reagent, tap plate to mix. Incubate 20 min.3. Read fluorescence at lex=530nm and lem=585nm.Notes: If the calculated galactose concentration of a sample is higher than 1000uM in Colorimetric Assay: or 100uM in Fluorometric Assay:, dilute sample in water and repeat the assay. Multiply result by the dilution factor n.Calculation:Subtract blank value (water, #8) from the standard values and plot the DOD or DRFU against standard concentrations. Determine the slope and calculate the galactose concentration of Sample,[Galactose] =ODSAMPLE–ODH2OSlope× n (uM)Colorimetry: [Galactose] =RFUSAMPLE–RFUH2OSlope× n (uM)Fluorimetry: ODSAMPLE, ODH2O are optical density values of the sample and water. RFUSAMPLE, RFUH2O are fluorescence intensity values of the sample and water. n is the dilution factor.Conversions: 1mM galactose equals 18 mg/dL, 0.018% or 180 ppm.Materials Required, But Not Provided:Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, optical density plate reader; black 96-well plates and fluorescence plate reader.
产品特点
针对Galactose Assay Kit, BioAssay该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Galactose Assay Kit, BioAssay产品后将其置于-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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