欢迎您访问武汉艾美捷科技有限公司官方网站!
站点地图 服务热线:400-6800-868
  • 产品
  • 文章

PRODUCT CENTER

细胞分析试剂盒
产品中心

当前位置:首页 > 产品中心 > 细胞生物学产品 > 细胞分析试剂盒

中文名称

FLRG, Human, BioAssay ELISA Kit (Follistatin-related Protein 3, Follistatin-like Protein 3, Follistatin-related Gene Protein, FSTL3, UNQ674/PRO1308)

英文名字
FLRG, Human, BioAssay ELISA Kit (Follistatin-related Protein 3, Follistatin-like Protein 3, Follistatin-related Gene Protein, FSTL3, UNQ674/PRO1308)
供应商
USBiological
产品货号
F9090-05
产品报价
¥1/1Kit
产品说明书
点击查看
购买方式
银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868
产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Follistatin-related Gene (FLRG), also known as Follistatin-like 3 (FSTL3) and Follistatin-related protein (FSRP), is a 30-35kD secreted glycoprotein that exerts a broad range of activities by neutralizing Activin A and Myostatin function. Mature human FLRG consists of an Activin and Myostatin binding N-terminal domain, two Follistatin-like domains, and two Kazal-like domains. BMP-2, -6, and -7 do not compete for Activin A binding, and FLRG binds only weakly to Activin B. Unlike Follistatin, FLRG does not contain a heparin-binding domain and does not interact with heparan sulfate proteoglycans. A short form of FLRG, which lacks the signal sequence and is found in the nucleus, retains Activin-blocking capability. Nuclear FLRG binds to the transcription factor AF10, induces AF10 homo-oligomerization, and enhances AF10-dependent gene transcription. Mature human FLRG shares 84% aa sequence identity with mouse and rat FLRG. FLRG expression is induced by Activin A, providing negative feedback regulation of Activin A function. FLRG is expressed in the uterus, ovary, testis, placenta, trophoblast, and adrenal gland. It is upregulated in cardiac myocytes following myocardial injury and blocks the prosurvival and hypertrophic effects of Activin A on these cells. FLRG is also expressed by astroglial cells in areas of neuronal trauma. Its binding to ADAM12 as well as to Activin A inhibits osteoclastic differentiation from macrophages. The Activin-neutralizing function enables FLRG to regulate energy balance and metabolism, as shown in FLRG knockout mice which exhibit increased pancreatic islet number and size, improved glucose tolerance and insulin sensitivity, and a shift from visceral to subcutaneous fat. Obese ob/ob mice show FLRG downregulation in visceral fat and upregulation in subcutaneous fat relative to wild type mice. Circulating FLRG levels are decreased in women with gestational diabetes. FLRG is upregulated in breast cancer and promotes tumor cell proliferation. The FLRG, Human, BioAssay ELISA Kit is a 4.5h solid-phase ELISA designed to measure human FLRG in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human FLRG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FLRG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FLRG.Sample Type:Cell culture supernates, serum, plasma, urine, and human milk.Intended Use:For the quantitative determination of Follistatin-related Gene (FLRG) concentrations in cell culture supernates, serum, plasma, urine, and human milk.Sensitivity:~3.68pg/mlRange:2.01-8.55pg/mlSpecificity:Human FLRGTest Principle:This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for FLRG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FLRG present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for FLRG is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FLRG bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:FLRG Microplate: 96 well polystyrene microplate (12 strips x 8 wells) coated with a monoclonal antibody to FLRG.FLRG Conjugate: 1x21ml of monoclonal antibody to FLRG conjugated to HRP with preservatives.FLRG Standard: 40ng of recombinant human FLRG in a buffered protein base with preservatives; lyophilized.Assay Diluent RD1W: 1x11ml of of a buffered protein base with preservatives.Calibrator Diluent RD5-24: 1x21ml of a buffered protein base with preservatives.Wash Buffer Concentrate: 1x21ml of a 25-fold concentrated solution of buffered surfactant with preservativesColor Reagent A: 1x12ml of stabilized hydrogen peroxideColor Reagent B: 1x12ml of stabilized chromogen (tetramethylbenzidine)Stop Solution: 1x6ml of 2 N sulfuric acidPlate Sealers: 4 adhesive stripsStorage and Stability:See Kit Protocol for detailed storage instructions.
产品特点
针对FLRG, Human, BioAssay ELISA Kit (Follistatin-related Protein 3, Follistatin-like Protein 3, Follistatin-related Gene Protein, FSTL3, UNQ674/PRO1308)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到FLRG, Human, BioAssay ELISA Kit (Follistatin-related Protein 3, Follistatin-like Protein 3, Follistatin-related Gene Protein, FSTL3, UNQ674/PRO1308)产品后将其置于-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
在线留言
  • 高品质保障 成熟的生产研发技术
  • 高性价比 价宜质优,性价比高
  • 高效省心 从购买到使用,放心无忧
  • 安全运输 完善的保护措施安全运输

微信扫码在线客服

微信咨询

全国免费技术支持

400-6800-868

在线客服