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细胞分析试剂盒
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中文名称

Fructose Assay Kit, BioAssay

英文名字
Fructose Assay Kit, BioAssay
供应商
USBiological
产品货号
F8000-11
产品报价
¥1/100 Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Fructose (C6H12O6, also called levulose or laevulose), is a monosaccharide found in honey, tree fruits, berries, melons, and some root vegetables along with glucose and galactose. The human body can use fructose for energy, however, too much consumption may lead to high triglycerides. Simple, direct and high-throughput assays for fructose determination find wide applications. This Fructose Assay Kit uses reagents that react directly and specifically with fructose to form a colored product. Glucose and galactose do not interfere. The color intensity at 565nm is directly proportional to the fructose concentration in the sample. As little as 20ul of sample can be used. The linear detection range (in 96-well plate) is 12-1000uM fructose.Applications:Direct Assays: Fructose determination in biological samples (e.g. serum, plasma, urine, saliva, milk, culture medium), food, juice, beverage and other agricultural products.Drug Discovery/Pharmacology: Effects of drugs on fructose metabolism.Kit Components:F8000-11A: Enzyme (lyophilized), 1x1 vial F8000-11B: Enzyme Buffer, 1x150ulF8000-11C: Standard D-Fructose (20mM), 1x400ulF8000-11D: Assay Buffer, 1x10mlF8000-11E: PMS Solution, 1x1.5mlF8000-11F: MTT Solution, 1x1.5mlMaterials Required But Not Provided:• Pipetting devices• Centrifuge tubes• Clear flat-bottom uncoated 96-well plates• Optical density plate readerStorage and Stability:Store all kit components at -20C. Kit is stable for 6 months after receipt.Precautions:This kit is for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents.Procedural Notes:1. The following substances interfere and should be avoided in sample preparation: ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%). 2. This assay is based on a kinetic reaction. To ensure identical incubation time, addition of Working Reagent to standard and samples should be quick and mixing should be brief but thorough. Use of a multi-channel pipettor is recommended.Reagent Preparation:Reconstitute Enzyme by adding 120ul Enzyme Buffer to the Enzyme tube. Make sure Enzyme is fully dissolved by pipetting up and down. Store reconstituted Enzyme at -20°C and use within 2 months.Sample Treatment:Liquid samples such as serum, plasma and fruit juices can be assayed directly. Because fruit juices may contain high concentrations of fructose, it is recommended to dilute juice sample 50-fold (n = 50) in dH2O prior to assay. Milk samples should be cleared by mixing 600ul milk with 100ul 6N HCl. Centrifuge 5 min at 14,000rpm. Transfer 300ul supernatant into a clean tube and neutralize with 50ul 6N NaOH. The neutralized supernatant is ready for assay (dilution factor n = 1.36).Assay Procedure:1. Equilibrate all components to room temperature. Briefly centrifuge the tubes before opening. Keep thawed tubes on ice during assay.2. Standards: Mix 12ul 20mM Standard with 228ul dH2O (final concentration: 1000uM). Dilute standard in dH2O as follows:No1000uM STD + H2OVol (ul)Fructose (uM)1100ul + 0ul10010002 60ul + 40ul100 6003 30ul + 70ul100 3004 0ul + 100ul100 0Transfer 20ul diluted standards into separate wells of a clear flat- bottom 96-well plate.Samples: Transfer 20ul of each sample into separate wells of the plate.3. Color reaction. Prepare enough Working Reagent by mixing, for each reaction well, 56ul Assay Buffer, 1ul reconstituted Enzyme, 14ul PMS Solution and 14ul MTT Solution.Keep Working Reagent protected from light. Add 80ul Working Reagent to each well. Tap plate to mix. Do not expose Working Reagent to light for more than 5 minutes. Incubate 60 min at room temperature in the dark.4. Read optical density at 565nm (520-600nm).Note: If the calculated fructose concentration of a sample is higher than 1000uM, dilute sample in water and repeat the assay. Multiply result by the dilution factor n.Calculation:Subtract blank value (water, #4) from the standard values and plot the ΔOD against standard concentrations. Determine the slope and calculate the fructose concentration of Sample:Fructose concentration (uM) = [(ODSample - ODH2O)/ Slope (uM-1)] x n where:ODSample and ODH2O are optical density values of the sample and water, respectively. n is the dilution factorConversions: 1mM fructose equals 18mg/dL, 0.018% or 180ppm.
产品特点
针对Fructose Assay Kit, BioAssay该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Fructose Assay Kit, BioAssay产品后将其置于-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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