A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 （Dehio et al.，1999； GenBank accession No. AF106020） was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A，a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus （OV） （Lyttle et al.，1994）. Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins （Dehio et al.，1999； Wise et al.，1999）. Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A，VEGF-E was found to bind with high affinity to VEGF receptor-2 （KDR） resulting in receptor autophosphorylation，whilst in contrast to VEGF-A，VEGF-E can not bind to VEGF receptor-1 （Flt-1）. Furthermore VEGF-E can also not bind to VEGF receptor-3 （FLT-4）. Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.
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大肠杆菌中重组表达
溶解建议
提供的血管内皮生长因子E （Orf Virus）（VEGF E （Orf Virus））产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml血管内皮生长因子E （Orf Virus）（VEGF E （Orf Virus））的PBS（pH 7.4）溶液中冻干产生的.冻干的血管内皮生长因子E具有非常好的水溶性.我们建议加PBS或培养液（0.1% 人或牛血清白蛋白）以配制工作储存液到不低于50ug/ml.
保存建议
血管内皮生长因子E （Orf Virus）（VEGF E （Orf Virus））在室温可保存3周，或置于-18℃冻存用于长期保存.重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装后置于-18℃冻存.请避免反复冻融.
