- 中文名称
c-Jun, Total, ELISA Kit (Antibodies Only) discontinued
- 英文名字
- c-Jun, Total, ELISA Kit (Antibodies Only) discontinued
- 供应商
- USBiological
- 产品货号
- C5815-10G
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Total c-Jun ELISA Kit (Antibodies Only) is being offered as an alternative to our Total c-Jun Sandwich ELISA Kit (C5815-10H). Capture and Detection antibodies (100X stocks) and a HRP-Conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The c-Jun capture antibody in PBS is coated overnight in a 96 well microplate. After blocking, cell lysates are added, followed by c-Jun detection antibody and HRP-Conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of total c-Jun protein.c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component ofthe transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (Reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9 ). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun vival has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11-13).Species CrossreactivityHuman, Mouse and RatKit ComponentsC5815-10G1: Total c-Jun Capture Antibody (100X), 1x400ul. Pink cap.C5815-10G2: Total c-Jun Detection Antibody (100X), 1x400ul. Blue cap.C5815-10G3: IgG-HRP Antibody (1000X), 1x40ul. Anti-mouse IgG conjugated to HRP. Yellow cap.Materials required but not suppliedPhosphate Buffered Saline (PBS)Phosphate Buffered Saline (PBS) with Tween-20TMB SubstrateStop SolutionBlocking Buffer-PBS containing 0.05% Tween-20 and 1% BSA96-well microplates (Corning® 96 Well Clear Polystyrene High Bind Stripwell Microplates (#2592) or Corning® 96 Well EIA/RIA Easy Wash Clear Flat Bottom Polystyrene High Bind Microplates (#3369))Microplate readerStorage and StabilityStore C5815-10G1 and C5815-10G2 at 4°C. Store C5815-10G3 at -20°C. Kit is stable for up to 6 months.
- 产品特点
- 针对c-Jun, Total, ELISA Kit (Antibodies Only) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到c-Jun, Total, ELISA Kit (Antibodies Only) discontinued产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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