This Rac1 G-LISA™ is a colorimetric based assay and shares the many advantages that all G-LISA™ activation assay have. This assay is specific for Rac1.
The Rac1 G-LISA™ kit contains a Rac-GTP-binding protein linked to the wells of a 96 well plate. Active GTP-bound Rac1 is captured by this protein while inactive GDP-bound Rac1 is removed following washes. The active Rac1 bound to the wells is detected with a Rac1 specific antibody. The degree of Rac1 activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.
